The location of non-specific esterase in human lung macrophages

Jaubert, Françis; Monnet, Jean-Paul; Danel, Claire; Chrétien, J; Nézelof, C

doi:10.1007/bf00496536

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…The circumferential distribution of the reaction product in monocytes explains its diffuse appearance under the light microscope. A similar distribution has been observed in human alveolar macrophages by Jaubert et al (30). The reactive intracellular bodies in lymphocytes correspond with the punctate densities or focal reaction product seen by light microscopy and seem to correspond with organelles called Gall bodies (see below).…”

Section: Discussionsupporting

confidence: 80%

Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.

Bozdech¹,

Bainton²

1981

The Journal of Experimental Medicine

106

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A reaction for an esterase, with a nonhalogenated, short-chain naphthyl ester (alpha-naphthyl butyrate or alpha-naphthyl acetate) as the substrate, has been used to identify mononuclear phagocytes by light microscopy. By analyzing techniques used in the collection, separation, fixation, processing, and embedding of human blood leukocytes for electron microscopy, we adapted the light microscopic method for use in determining the fine structural localization of this reaction. In monocytes, the reaction product covered the external surface of the plasma membrane. This distribution indicated that monocytic esterase is an ectoenzyme. The addition of NaF completely inhibited the monocytic reaction. In lymphocytes, the reaction product was localized in membrane-bounded intracellular organelles, similar to those previously shown to contain phospholipid and called Gall bodies. These organelles correspond with the punctate densities or focal reaction product observed by light microscopy. Other investigators believe that this distribution of enzyme in lymphocytes marks a subset of T cells, the Tmicro. The lymphocytic reaction was not inhibited by NaF.

show abstract

Section: Discussionsupporting

confidence: 80%

Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.

Bozdech¹,

Bainton²

1981

The Journal of Experimental Medicine

106

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show abstract

“…In this regard, it is interesting that Ling et al (1982) found that NSE activity was associated with lysosomes in amoeboid microglia in the neonatal rat brain. However, most studies that have examined the subcellular localization of NSE have not found it in lysosomes but rather describe it as associated with the plasma membrane, possibly as an ectoenzyme, as well as with internal membrane systems (Sannes and Randell, 1985;Akikusa et al, 1984;Monahan et al, 1981;Jaubert et al, 1978Jaubert et al, ,1980. On the basis of these observations, it has been suggested that NSE activity may be involved in such functions as inactivation of toxic products and microorganisms and the killing of cells expressing foreign antigens (Monahan et al, 1981;Nelson et al, 1981;Buchmuller and Mauel, 1980).…”

Section: Discussionmentioning

confidence: 99%

Nonspecific esterase activity in astrocytes of the goldfish brain.

Pepperell¹,

Levine²

1996

J Histochem Cytochem.

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We examined the distribution of nonspecific esterase (NSE) activity in the brain of the goldfish, Carawius auracus, and found that the enzyme is expressed at high levels in cells that appeared to be radial astrocytes. Several instances in which neurons expressed NSE activity were also seen. To confirm the identity of the radial profiles as astrocgtes, similar sections were labeled with antiserum against goldfiih glial fibrillary acidic protein (GFAP). The concordance between the NSE and the anti-GFAP data in both the visual system and the telencephalon was essentially complete, confirming that the NSE reaction was labeling astrocytes in these structures. The two methods also gave similar results in both the cerebellum and the vagal lobes, although the concordance between them in these instances was somewhat less complete.

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The location of non-specific esterase in human lung macrophages

Cited by 2 publications

References 11 publications

Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.

Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.

Nonspecific esterase activity in astrocytes of the goldfish brain.

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