Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.

Bozdech, Marek J.; Bainton, Dorothy F.

doi:10.1084/jem.153.1.182

Cited by 106 publications

(39 citation statements)

References 32 publications

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“…There is some evidence that it is related to the immune response, especially cytotoxicity. 26 Although reported to be a membrane bound ecto-enzyme, 27 its mechanism of release from the cell is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of polycarbonate‐based polyurethanes by the human monocyte‐derived macrophage and U937 cell systems

Matheson

Labow

Santerre

2002

J. Biomed. Mater. Res.

101

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The prominent cell type found on implanted medical devices during the chronic inflammatory response is the monocyte-derived macrophage (MDM). Using an activated in vitro cell system, it was possible to show that MDMs possess esterolytic activities that may contribute to the degradation of polyurethanes. In the present study, the U937 cell line was paralleled to the MDM cell system in order to validate the use of a cell line that could expedite studies on biomaterial biocompatibility and biostability. Using 12-o-tetradecanoylphorbol 13-acetate (PMA), the optimum differentiation time for the U937 cells was 72 h based on biodegradation, degradative potential, and (35)S-methionine uptake. After activation of the cells by resuspending from tissue culture polystyrene plates and reseeding onto a (14)C-labeled polycarbonate-based polyurethane(PCNU), both U937 cells and the MDMs elicited comparable radiolabel release (measure of polymer breakdown) and esterase activity (measure of degradative potential) at 48 h. There was no difference in the effect on radiolabel release and esterase activity elicited by both cell types with inhibitors of protein synthesis, esterase activity, and phospholipase A(2). This established that both cell types likely used similar hydrolytic activities and signaling pathways to cause degradation of the PCNU. Immunoblotting demonstrated that both cell systems secreted monocyte-specific esterase and cholesterol esterase enzymes previously shown to degrade PCNUs. The U937 cell system is more convenient and reproducible than MDMs for pursuing possible biological pathways elucidating the mechanism of polyurethane biodegradation. Once established with U937s, the pathways can then be validated with the more physiologically relevant human MDM cell system.

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Section: Discussionmentioning

confidence: 99%

Biodegradation of polycarbonate‐based polyurethanes by the human monocyte‐derived macrophage and U937 cell systems

Matheson

Labow

Santerre

2002

J. Biomed. Mater. Res.

101

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“…In man, lymphocytes with this type of staining have been identified by E-rosetting as Tcells. '-I 1 , 1 6 , 2 7 The reaction product corresponds to ultrastructural membrane-bound organelles called Gall bodies7 thought to specifically mark Tu cells, those able to bind the Fc portion of IgM. 17 Cytochemical staining was a valuable adjunct to the morphologic characterization of various leukemias.…”

Section: Discussionmentioning

confidence: 99%

Cytochemical Characterization of Feline Leukemic Cells

Facklam

Kociba

1986

Vet Pathol

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Abstract. Hematopoietic cells in blood and/or bone marrow from 23 leukemic cats and ten control cats were characterized using a battery of cytochemical enzyme stains. The results of cytochemical staining led to modification of diagnosis based on clinical, hematologic and histopathologic findings in four (1 7%) of the leukemias. Sudan black-B and chloroacetate esterase served as granulocytic markers in both the control and leukemic groups. Peroxidase activity was seen in the granulocytes and monocytes of control animals but not in the blasts of leukemic cats. Diffuse alpha naphthyl butyrate esterase staining marked monocytes in both control and leukemic animals. Cytochemical staining was found to be a valuable aid in the classification of leukemias in the cat. Materials and MethodsBone marrow specimens were aspirated from the proximal humerus or femur from leukemic cats presented to the Ohio University Veterinary Teaching Hospital. Samples were also collected from ten control cats including patients with nonhematologic diseases. In some cases, blood samples collected on the same day as the marrow aspirate also were stained. A few samples were obtained from a private veterinary laboratory. Blood films and bone marrow coverslip smears were prepared and air-dried generally within one hour of sample collection. Films were stained with Wright-Giemsa, alpha naphthyl butyrate esterase," and periodic a~id-Schiff.~~ Cytochemical staining kits (Sigma Chemical Company, St. Louis, MO) were used for the Sudan black-B, acid phosphatase with and without tartrate, alkaline phosphatase, and chloroacetate esterase procedures. The chloroacetate esterase procedure was modified by increasing the incubation temperature to 37 C.A Dositive control was included in each batch of stains. Posphatase and alkaline phosphatase which were fixed in accordance with the manufacturer's instructions (Sigma Chemical Company, St. Louis, MO) and stored at 4 C. The corresponding histologic sections of representative tissues from animals that were necropsied along with the blood and bone marrow films were reviewed by one of the authors. Diagnosis was based on cell morphology, anatomic distribution of lesions, and histologic findings. '3,'5,L8,19,28 Due to the paucity of specific diagnostic criteria for feline leukemias, the lymphocytic leukemias were subdivided only into lymphoblastic and well-differentiated lymphocytic categories.28 These results were compared with the corresponding cytochemical findings. ResultsThe cytochemical staining characteristics of normal cells (Table 1)

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“…The second candidate, carboxylesterase D1, is a serine esterase that is highly similar to human monocyte/macrophage serine esterase 1 [17] . Esterase activity in human monocytes has been demonstrated to correlate with chemotaxis [18] . Histone deacetylase complex subunit SAP18 is a transcription factor.…”

Section: Differentially Regulated Proteins In the Uveitis Target Organsmentioning

confidence: 99%

Equine Recurrent Uveitis – A Spontaneous Horse Model of Uveitis

Deeg

Hauck²,

Amann

et al. 2008

Ophthalmic Res

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Equine recurrent uveitis (ERU) is an autoimmune disease that occurs with a high prevalence (10%) in horses. ERU represents the only reliable spontaneous model for human autoimmune uveitis. We already identified and characterized novel autoantigens (malate dehydrogenase, recoverin, CRALBP) by analyzing the autoantibody-binding pattern of horses affected by spontaneous recurrent uveitis (ERU) to the retinal proteome. CRALBP also seems to be relevant to human autoimmune uveitis. Proteomic screening of vitreous and retinal samples from ERU diseased cases in comparison to healthy controls has led to the identification of a series of differentially regulated proteins, which are functionally linked to the immune system and the maintenance of the blood-retinal barrier.

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Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.

Cited by 106 publications

References 32 publications

Biodegradation of polycarbonate‐based polyurethanes by the human monocyte‐derived macrophage and U937 cell systems

Biodegradation of polycarbonate‐based polyurethanes by the human monocyte‐derived macrophage and U937 cell systems

Cytochemical Characterization of Feline Leukemic Cells

Equine Recurrent Uveitis – A Spontaneous Horse Model of Uveitis

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