2002
DOI: 10.1002/jbm.10286
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Abstract: The prominent cell type found on implanted medical devices during the chronic inflammatory response is the monocyte-derived macrophage (MDM). Using an activated in vitro cell system, it was possible to show that MDMs possess esterolytic activities that may contribute to the degradation of polyurethanes. In the present study, the U937 cell line was paralleled to the MDM cell system in order to validate the use of a cell line that could expedite studies on biomaterial biocompatibility and biostability. Using 12-… Show more

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Cited by 66 publications
(103 citation statements)
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“…[89] Studies of this phenomenon by Labow and Santerre have focused on the ability of macrophage-derived enzymes to facilitate degradation of polyurethanes. [90][91][92][93][94][95] These studies clearly identified the importance of the use of antioxidants in these polymers to inhibit the oxidation process that occurs with the foreign body reaction. The persistence of the foreign body reaction and the fact that it is present at the interface between the tissue and the device for the lifetime of the device, suggests that the oxidation process is continuous, albeit at low levels.…”
Section: Device Failurementioning
confidence: 92%
See 1 more Smart Citation
“…[89] Studies of this phenomenon by Labow and Santerre have focused on the ability of macrophage-derived enzymes to facilitate degradation of polyurethanes. [90][91][92][93][94][95] These studies clearly identified the importance of the use of antioxidants in these polymers to inhibit the oxidation process that occurs with the foreign body reaction. The persistence of the foreign body reaction and the fact that it is present at the interface between the tissue and the device for the lifetime of the device, suggests that the oxidation process is continuous, albeit at low levels.…”
Section: Device Failurementioning
confidence: 92%
“…The soft segment of polyether polyurethanes is known to undergo degradation. [86][87][88][89][90][91][92][93][94][95][96][97][98] This phenomenon will be discussed in the next section.…”
Section: Mediators Of Degradation-mentioning
confidence: 97%
“…The U937 cell line was originally derived from a patient with diffuse histiocytic lymphoma. 19 These cells can be stimu- 20 Mf-free cultures containing B50% preadipocytes and B50% adipocytes, based on visual observations and previous analyses, 21 were treated between day 6-12 of differentiation. Each experiment was repeated at least twice at different times using a mixture of cells from 2-3 subjects unless otherwise indicated.…”
Section: Culturing Of Human Mfsmentioning
confidence: 99%
“…16 The primary antibody used was rabbit anti-porcine esterase (reacts with MSE) or rabbit anti-CE (a gift from Dr. Hui, University of Cincinnati College of Medicine, Cincinnati, OH). The secondary antibody used was goat antirabbit immunoglobulin G conjugated to horseradish peroxidase.…”
Section: Sodium Dodecyl Sulfate Gel Electrophoresis and Immunoblottingmentioning
confidence: 99%
“…7,16 The total esterase activity, de novo esterase synthesis, and amount of both CE and MSE in MDMs differed depending on the material surface. 17 The above studies clearly indicated that macrophages respond to small changes in polymer chemistry depending on which PCNU is the seeding surface for the MDMs; however, the mechanism of macrophage activation by these different materials is still unknown.…”
Section: Introductionmentioning
confidence: 99%