The location of aluminium in protoplasts and suspension cells taken from Coffea arabica L. with different tolerance of Al

Ramírez-Benítez, José Efraín; Hernández‐Sotomayor, S.M. Teresa; Muñoz-Sánchez, J. Armando

doi:10.1016/j.jinorgbio.2009.07.016

Cited by 7 publications

(7 citation statements)

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“…Verificou-se diferença significativa para o crescimento do sistema radicular, em relação ao tipo de solo, sendo obtido maior crescimento das plantas cultivadas em solo de várzea. Altos teores de alumínio, como os observados no solo de terras altas utilizado neste estudo, exercem efeitos tóxicos sobre o crescimento dos vegetais, notadamente sobre o sistema radicular, reduzindo ou inibindo a divisão celular (RAMÍREZ-BENÍTEZ et al, 2009), bem como diminuindo a absorção e a translocação de fósforo, cálcio e magnésio na planta e, consequentemente, a produtividade da cultura (FREITAS et al, 2006). Fonte: Santos, L. A. C. et al (2013).…”

Section: Resultsunclassified

Growth of cowpea cultivars in upland and lowland soils

Santos¹,

Silva²,

Oliveira³

et al. 2017

Ambiência

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ResumoO feijão caupi apresenta grande importância na alimentação, especialmente das populações das regiões norte e nordeste brasileiro, sendo uma das principais fontes de energia e proteína nessas localidades. Entretanto, as produtividades obtidas geralmente são baixas, o que poderia ser contornado utilizando-se solos de maior fertilidade natural. Assim, o objetivo, neste trabalho, foi avaliar o crescimento de três cultivares de feijão caupi cultivado em solo de terra firme e de várzea, na região de Humaitá, Amazonas. O experimento foi conduzido em casa de vegetação, utilizando-se sacos de polietileno de aproximadamente 2 litros. Como substrato, foram coletadas amostras de solos na profundidade 0,0 a 0,20 m, de Argissolo Vermelho (terra firme) e Neossolo Flúvico (várzea). Foram utilizadas, na semeadura, as cultivares: Barrigudinho, Manteiguinha e Felipinho. O delineamento estatístico utilizado foi em blocos completos casualizados, em esquema fatorial 2x3, com quatro repetições. Foram avaliadas as seguintes características: altura da planta, diâmetro do coleto, biomassa seca e verde da parte aérea e radicular. Verificou-se que o solo de várzea é mais propício ao cultivo do

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Section: Resultsunclassified

Growth of cowpea cultivars in upland and lowland soils

Santos¹,

Silva²,

Oliveira³

et al. 2017

Ambiência

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“…The LS was sterilized by filtration using Millex syringe-driven filter units (Millipore Corp., USA) with a 0.22 µm pore size. The mixture was later incubated on a rotator shaker (50 rpm) for 12 h in the dark at 28 ± 2 °C for protoplast isolation (Ramírez-Benítez et al 2009). Rifampicin (4 mg l −1 ) and leupeptin (1 μg ml −1 ) were added to the LS prior to filtering.…”

Section: Methodsmentioning

confidence: 99%

“…These types of studies also avoid complications that might arise due to interactions between Al 3+ and cell wall components. Ramírez-Benítez et al (2009) found that after applying a 100 μM AlCl 3 treatment to coffee protoplasts for short periods of time, aluminum passed through the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Protoplasts: a friendly tool to study aluminum toxicity and coffee cell viability

et al. 2016

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ObjectiveAluminum toxicity is a major limiting factor with regard to crop production and quality in most acidic soils around the world. We propose the use of C. arabica L. protoplasts to evaluate the toxic effects of aluminum, the nuclear localization of aluminum and propensity of aluminum to cause DNA damage.ResultsAfter protoplasts were exposed to aluminum (Al) for varying periods of time (0, 5, 10, 20 and 30 min), we detected a reduction in protoplast viability. Additionally, we observed a rapid decline in the ability of protoplasts to synthesize DNA following exposure to Al for 30 min. Furthermore, DNA damage was observed after 10 min of treatment with Al.ConclusionsProtoplasts can be used to evaluate the effects of Al upon entry into the cell, which affects the structure of the nucleus. These results indicate that protoplasts provide a useful model for the study Al toxicity at the cellular level.

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“…Coffea is the most economically important genus of Rubiaceae family and is a very important crop in several tropical countries (Ramírez-Benítez et al 2009). In Brazil, one of the largest coffee exporter in the world, 40% of coffee plantations are established in soils with low pH (3.7 to 5.0), with high Al content (Rodrigues et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the aim of this work was to study the effects of low Al concentrations on the enzyme antioxidant system, including ferritin, in coffee cell suspension cultures. Because we wanted to isolate the effects of Al from low pH soil, the medium pH was kept at 5.8, and the Al concentration was increased to a level (300 μM Al 3+ ) that was higher than the dose used in the literature for pH 4.3 (200 μM AlCl 3 ) (Ramírez-Benítez et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8

Bottcher

Nóbile

Martins

et al. 2012

Hoehnea

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-(Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8). The effects of aluminum (Al) on the activities of antioxidant enzymes and ferritin expression were studied in cell suspension cultures of two varieties of Coffea arabica, Mundo Novo and Icatu, in medium with pH at 5.8. The cells were incubated with 300 μM Al 3+ , and the Al speciation as Al 3+ was 1.45% of the mole fraction. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were increased in Mundo Novo, whereas glutathione reductase (GR) and guaiacol peroxidase (GPOX) activities remained unchanged. SOD, GR, and GST activities were increased in Icatu, while CAT activity was not changed, and GPOX activity decreased. The expression of two ferritin genes (CaFer1 and CaFer2) were analyzed by Real-Time PCR. Al caused a downregulation of CaFER1 expression and no changes of CaFER2 expression in both varieties. The Western blot showed no alteration in ferritin protein levels in Mundo Novo and a decrease in Icatu. The differential enzymes responses indicate that the response to Al is variety-dependent.

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The location of aluminium in protoplasts and suspension cells taken from Coffea arabica L. with different tolerance of Al

Cited by 7 publications

References 50 publications

Growth of cowpea cultivars in upland and lowland soils

Growth of cowpea cultivars in upland and lowland soils

Protoplasts: a friendly tool to study aluminum toxicity and coffee cell viability

Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8

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