Protoplasts: a friendly tool to study aluminum toxicity and coffee cell viability

Poot-Poot, Wilberth Alfredo; Rodas-Junco, Beatriz A.; Muñoz-Sánchez, J. Armando; Hernández‐Sotomayor, S.M. Teresa

doi:10.1186/s40064-016-3140-2

Cited by 7 publications

(7 citation statements)

References 47 publications

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“…The MDA content in date palm callus treated with Al at 200 mg.l -1 was increased by up to 1.71-fold compared with that in the control callus. Lipid peroxidation was also induced under Al stress [14, 19, 27, 35]. The increase in lipid peroxidation may be attributed to the binding of Al to biomembrane and leads to rigidity, which in turns causes the generation of radical chain reactions by Fe ions [21, 48].…”

Section: Discussionmentioning

confidence: 99%

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Awad

Salih

Khalaf

et al. 2019

Journal of Genetic Engineering and Biotechnology

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BackgroundAl is a common metallic element found in earth's crust and is a toxic pollutant present at high concentrations in acidic soil, thus affecting plant growth. Despite being well studied as a toxic element, the effects of Al on date palm have not been investigated. This study aimed to assess the toxic effects of different Al concentrations on the development and growth of date palm callus and evaluate the biochemical and molecular response of date palm cells under Al stress.ResultsOur study revealed the phytotoxicity of Al concentrations (50, 100, 150 and 200 mg.l-1) on date palm callus. The fresh and dry weight and the number of produced embryos were significantly decreased in response to Al concentration. At 150 mg.l-1, the embryo number decreased to 1.66 compared with the 19.33 in the control treatment. At high Al concentration (200 mg.l-1), the callus failed to produce any embryo. Biochemical analysis revealed that Al exposure had negative effect on callus. Total soluble carbohydrates, total soluble protein and free amino acids were decreased in plants receiving 200 mg.l-1 Al treatment compared with those in the untreated ones. A similar decline was observed in total soluble protein and free amino acid in response to Al treatment. Significant accumulations of malondialdehyde, H2O2 and peroxidase activity accompanied the increase in Al concentration in cultured tissues, revealing the generation of toxic reactive oxygen species in affected cultures. The genotoxic effect of Al at high concentrations (150 and 200 mg.l-1) was revealed by protein patterns.ConclusionOur findings revealed for the first time the phytotoxicity of Al to date palm callus. At 200 mg.l-1, Al prevented the embryo production of date palm callus. At 50, 100, 150 and 200 mg.l-1, Al negatively affected the biochemical characteristics of date palm callus. At 150 and 200 mg.l-1, Al induced changes in protein expression. These data showed that the tissue culture technique can be used as a valuable approach in heavy metal toxicity studies.

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Section: Discussionmentioning

confidence: 99%

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Awad

Salih

Khalaf

et al. 2019

Journal of Genetic Engineering and Biotechnology

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“…Al 3+ ions are genotoxic and cause direct damage to DNA [ 69 ]. Sumoylation involves small ubiquitin-like modifiers (SUMOs), which are attached to or detached from proteins to modify their function and subcellular localization.…”

Section: Discussionmentioning

confidence: 99%

Association of Proteomics Changes with Al-Sensitive Root Zones in Switchgrass

Rangu

Bhatti

et al. 2018

Proteomes

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In this paper, we report on aluminum (Al)-induced root proteomic changes in switchgrass. After growth in a hydroponic culture system supplemented with 400 μM of Al, plants began to show signs of physiological stress such as a reduction in photosynthetic rate. At this time, the basal 2-cm long root tips were harvested and divided into two segments, each of 1-cm in length, for protein extraction. Al-induced changes in proteomes were identified using tandem mass tags mass spectrometry (TMT-MS)-based quantitative proteomics analysis. A total of 216 proteins (approximately 3.6% of total proteins) showed significant differences between non-Al treated control and treated groups with significant fold change (twice the standard deviation; FDR adjusted p-value < 0.05). The apical root tip tissues expressed more dramatic proteome changes (164 significantly changed proteins; 3.9% of total proteins quantified) compared to the elongation/maturation zones (52 significantly changed proteins, 1.1% of total proteins quantified). Significantly changed proteins from the apical 1-cm root apex tissues were clustered into 25 biological pathways; proteins involved in the cell cycle (rotamase FKBP 1 isoforms, and CDC48 protein) were all at a reduced abundance level compared to the non-treated control group. In the root elongation/maturation zone tissues, the identified proteins were placed into 18 pathways, among which proteins involved in secondary metabolism (lignin biosynthesis) were identified. Several STRING protein interaction networks were developed for these Al-induced significantly changed proteins. This study has identified a large number of Al-responsive proteins, including transcription factors, which will be used for exploring new Al tolerance genes and mechanisms. Data are available via ProteomeXchange with identifiers PXD008882 and PXD009125.

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“…DNA damage evaluation is another frequently employed marker to assess protoplast viability or the effect of genotoxicity of environmental pollutants and abiotic stresses on protoplasts. In these procedures, protoplasts are used as a direct source of nuclei to perform gel electrophoresis with ethidium bromide staining in order to detect DNA laddering ( Poot-Poot et al, 2016 ). Identically, single cell gel electrophoresis assay (SCGE), also called Comet assay, allows the study of DNA damage on protoplasts at the single cell or nuclei level ( Kuzminsky et al, 2016 ; Badaró Costa et al, 2018 ; Choury et al, 2018 ).…”

Section: The Versatility Of Fluorescent Probes On Protoplastsmentioning

confidence: 99%

Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response

et al. 2021

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Plants are constantly facing abiotic and biotic stresses. To continue to thrive in their environment, they have developed many sophisticated mechanisms to perceive these stresses and provide an appropriate response. There are many ways to study these stress signals in plant, and among them, protoplasts appear to provide a unique experimental system. As plant cells devoid of cell wall, protoplasts allow observations at the individual cell level. They also offer a prime access to the plasma membrane and an original view on the inside of the cell. In this regard, protoplasts are particularly useful to address essential biological questions regarding stress response, such as protein signaling, ion fluxes, ROS production, and plasma membrane dynamics. Here, the tools associated with protoplasts to comprehend plant stress signaling are overviewed and their potential to decipher plant defense mechanisms is discussed.

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Protoplasts: a friendly tool to study aluminum toxicity and coffee cell viability

Cited by 7 publications

References 47 publications

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Association of Proteomics Changes with Al-Sensitive Root Zones in Switchgrass

Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response

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