BackgroundAl is a common metallic element found in earth's crust and is a toxic pollutant present at high concentrations in acidic soil, thus affecting plant growth. Despite being well studied as a toxic element, the effects of Al on date palm have not been investigated. This study aimed to assess the toxic effects of different Al concentrations on the development and growth of date palm callus and evaluate the biochemical and molecular response of date palm cells under Al stress.ResultsOur study revealed the phytotoxicity of Al concentrations (50, 100, 150 and 200 mg.l-1) on date palm callus. The fresh and dry weight and the number of produced embryos were significantly decreased in response to Al concentration. At 150 mg.l-1, the embryo number decreased to 1.66 compared with the 19.33 in the control treatment. At high Al concentration (200 mg.l-1), the callus failed to produce any embryo. Biochemical analysis revealed that Al exposure had negative effect on callus. Total soluble carbohydrates, total soluble protein and free amino acids were decreased in plants receiving 200 mg.l-1 Al treatment compared with those in the untreated ones. A similar decline was observed in total soluble protein and free amino acid in response to Al treatment. Significant accumulations of malondialdehyde, H2O2 and peroxidase activity accompanied the increase in Al concentration in cultured tissues, revealing the generation of toxic reactive oxygen species in affected cultures. The genotoxic effect of Al at high concentrations (150 and 200 mg.l-1) was revealed by protein patterns.ConclusionOur findings revealed for the first time the phytotoxicity of Al to date palm callus. At 200 mg.l-1, Al prevented the embryo production of date palm callus. At 50, 100, 150 and 200 mg.l-1, Al negatively affected the biochemical characteristics of date palm callus. At 150 and 200 mg.l-1, Al induced changes in protein expression. These data showed that the tissue culture technique can be used as a valuable approach in heavy metal toxicity studies.
A naphthalene degrading bacterium was isolated from diesel contaminated soil in Damietta County, Egypt. It is identified as Pseudomonas aeruginosa strain EGDS2 based on classical and 16S rDNA sequence techniques. P. aeruginosa EGDS2 was highly able to degrade naphthalene in addition to benzene, toluene, catechol, and xylene. The normalize dioxygenases of P. aeruginosa EGDS2 responsible for naphthalene degradation was optimized for producing the maximum activity. The results revealed that the maximum dioxygenases activity was after 48 hours at pH 9.0 and 30oC. The best nitrogen source was sodium nitrate, and 7g/l of naphthalene was the optimum concentration for highest dioxygenases activity. The promising yield of dioxygenases of P. aeruginosa EGDS2 makes this isolate very useful for bioremediation of aromatic hydrocarbon pollutants.
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