The Localization of Some Acid Phosphatases in Brain Tissue

Naidoo, D.; Pratt, O. E.

doi:10.1136/jnnp.14.4.287

Cited by 58 publications

(9 citation statements)

References 10 publications

(7 reference statements)

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“…Naidoo and Pratt (11) applied the Gomori method for acid phosphatase on freeze-dried, parqffin-embedded tissue. The cytoarchitecture of the cerebral cortex was well displayed as a consequence of neuronal staining.…”

Section: Introductionmentioning

confidence: 99%

The Localization of Enzyme Activities in the Rat Brain

Becker

Goldfischer

Shin

et al. 1960

The Journal of Cell Biology

102

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Studies with rat brain illustrate the usefulness of formol-calcium-fixed tissue for studying both enzymatic "chemoarchitectonics" and intracellular organelles. Unembedded frozen sections and polyvinyl alcohol-embedded sections may be used to demonstrate the activities of DPNH-tetrazolium reductase localized in mitochondria and ergastoplasm, TPNH-tetrazolium reductase localized in mitochondria, ATPase (and/or apyrase or ADPase) in cell membranes, and acid phosphatase in lysosomes. 1 Among the observations recorded are: (1) the presence of lysosomes in all cells of the brain; (2) the presence of numerous large lysosomes near the nuclei of capillary endothelial cells; (3) a polarized arrangement of large lysosomes in epithelial cells of the ependyma and choroid plexus; (4) the presence of ATPase activity in the cell membranes of some neurons; (5) the presence of either an apyrase or combination of ATPase and ADPase in the cell membranes of neuroglia and capillaries; (6) the presence of both DPNH-and TPNH-tetrazolium reductase activities in neuroglia; (7) the presence of DPNH-and TPNH-tetrazolium reductase activities in mitochondria and of DPNH-tetrazolium reductase activity in Nissl substance. The possible functional significance of these localizations is briefly discussed, as is their relation to "quantitative histochemistry" data available in the literature.

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Section: Introductionmentioning

confidence: 99%

The Localization of Enzyme Activities in the Rat Brain

Becker

Goldfischer

Shin

et al. 1960

The Journal of Cell Biology

102

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“…The change seen in the localization of staining due to the replacement of the aneurin pyrophosphate by another phosphoric acid ester, such as 5-adenylic acid, provides further confirmatory evidence that enzymatic dephosphorylation of aneurin pyrophosphate has been demonstrated. It precludes the possibility of the pictures being produced to any appreciable extent by the deposition of the precipitate on foci of crystallization within the tissue (Naidoo and Pratt, 1951).…”

Section: Resultsmentioning

confidence: 99%

“…The procedure employed for the preparation of sections was in no way different from that previously described for the localization of the sites of activity of phosphatases (Naidoo and Pratt, 1951). The site of enzymatic cleavage of the phosphoric acid ester used as the substrate was demonstrated by the precipitation of the liberated phosphate as an insoluble lead salt at the site of action.…”

Section: Methodsmentioning

confidence: 99%

The Localization of Aneurin Pyrophosphatase in the Brain

Naidoo¹,

Pratt²

1952

Journal of Neurology, Neurosurgery & Psychiatry

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“…Most of these observations have been largely confined to mammalian nervous tissue [Naidoo and Pratt, 1951: G omori and Chessik, 1953: Bourne, 1958: Anderson et at., 1962T ewari and Bourne, 1963], The neurology of the avian cerebellum and medulla oblongata has been discussed by C lark [1928], Craigii [1928] and Brodel et al [1950]. Some workers such as W hitlock [1952] and Aprison et at.…”

Section: Introductionmentioning

confidence: 99%

Localization of acid phosphatase activity in the medulla oblongata of the small brown dove, Streptopelia senegalensis, and white-throated munia, Uroloncha malabarica

Singh¹

1976

Cells Tissues Organs

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In the present study, acid phosphatase activity has been histochemically localized in the medulla oblongata (facialis part) of Streptopelia senegalensis and Uroloncha malabarica. It was noticed that the enzyme is present particularly in the motor nuclei and lysosomes of neuroglial cells of the medulla oblongata. However, enzymatic deposition appears to be somewhat more vigorous in S. senegalensis than in U. malabarica, thereby indicating their respective metabolic turnover and neuronal excitability. Simultaneously, the functional significance has been discussed in the light of differential localization.

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The Localization of Some Acid Phosphatases in Brain Tissue

Cited by 58 publications

References 10 publications

The Localization of Enzyme Activities in the Rat Brain

The Localization of Enzyme Activities in the Rat Brain

The Localization of Aneurin Pyrophosphatase in the Brain

Localization of acid phosphatase activity in the medulla oblongata of the small brown dove, <i>Streptopelia senegalensi</i><i>s</i>, and white-throated munia, <i>Uroloncha malabaric</i><i>a</i>

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