The localization of phototropin to the plasma membrane defines a cold-sensing compartment in <i>Marchantia polymorpha</i>

Hirano, Satoyuki; Sasaki, Kotoko; Osaki, Yasuhide; Tahara, Kyoka; Takahashi, Hiroyuki; Takemiya, Atsushi; Kodama, Yutaka

doi:10.1093/pnasnexus/pgac030

Cited by 7 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The distinct localisation of Aephot-Citrine in M. polymorpha could potentially explain why it did not promote the signaling pathway because the avoidance response under cold conditions was reported to be initiated from the photoactivated phot at the plasma membrane. 26,33 Anyway, although cells expressing Aephot-Citrine did not exhibit to promote the signaling pathway (Figure 3A and Table 1), we observed that the plasma membrane-resident Aephot-Citrine in approx. 50% of the cells (Figure S4).…”

Section: Discussionmentioning

confidence: 85%

Functional comparison of phototropin from the liverworts Apopellia endiviifolia and Marchantia polymorpha

Yong,

Keino,

Kanna

et al. 2023

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

Phototropin (phot) is a blue light (BL) receptor and thermosensor that mediates chloroplast movements in plants. Liverworts, as early‐diverging plant species, have a single copy of PHOT gene, and the phot protein in each liverwort activates the signaling pathway adapted to its specific growing environment. In this study, we functionally compared phot from two different liverworts species: Apopellia endiviifolia (Aephot) and Marchantia polymorpha (Mpphot). The BL‐dependent photochemical activity of Aephot was similar to that of Mpphot, whereas the thermochemical activity of Aephot was lower than that of Mpphot. Therefore, the phot‐mediated signaling pathways of the two plant species may differ more in response to temperature than to BL. Furthermore, we analyzed the functional compatibility of Aephot and Mpphot in chloroplast movements by transiently expressing AePHOT or MpPHOT. The transient expression of AePHOT did not mediate chloroplast movement in M. polymorpha, showing the incompatibility of Aephot with the signaling pathway of M. polymorpha. By contrast, the transient expression of MpPHOT mediated chloroplast movement in A. endiviifolia, indicating the compatibility of Mpphot with the signaling pathway of A. endiviifolia. Our findings reveal both functional similarities and differences between Aephot and Mpphot proteins from the closely related liverworts.

show abstract

Section: Discussionmentioning

confidence: 85%

Functional comparison of phototropin from the liverworts Apopellia endiviifolia and Marchantia polymorpha

Yong,

Keino,

Kanna

et al. 2023

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Low temperatures can inactivate the functions of the plasma membrane, including destroying its lipid bilayer structure and negating its transport activities as well as basic metabolism [ 38 , 39 , 40 ]. To cope with low-temperature stress, plant membranes have evolved a variety of adaptive mechanisms, including changing their lipid composition and increasing sugar and soluble protein content [ 41 , 42 , 43 ]. In this process, plasma membrane proteins and lipids have been well described [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…To cope with low-temperature stress, plant membranes have evolved a variety of adaptive mechanisms, including changing their lipid composition and increasing sugar and soluble protein content [ 41 , 42 , 43 ]. In this process, plasma membrane proteins and lipids have been well described [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Identifies Novel Genes Associated with Low-Temperature Seed Germination in Sweet Corn

Xiao

Chen

Zheng

et al. 2022

Plants

View full text Add to dashboard Cite

Typically, sweet corn, particularly sh2 sweet corn, has low seed vigor owing to its high sugar and low starch content, which is a major problem in sweet corn production, particularly at low temperatures. There is considerable variation in the germination rates among sweet corn varieties under low-temperature conditions, and the underlying mechanisms behind this phenomenon remain unclear. In this study, we screened two inbred sweet corn lines (tolerant line L282 and sensitive line L693) differing in their low-temperature germination rates; while no difference was observed in their germination rates at normal temperatures. To identify the specifically induced genes influencing the germination capacity of sweet corn at low temperatures, a transcriptome analysis of the two lines was conducted at both normal and low temperatures. Compared to the lines at a normal temperature, 3926 and 1404 differently expressed genes (DEGs) were identified from L282 and L693, respectively, under low-temperature conditions. Of them, 830 DEGs were common DEGs (cDEGs) that were identified from both L282 and L693, which were majorly enriched in terms of microtubule-based processes, histone H3-K9 modification, single-organism cellular processes, and carbohydrate metabolic processes. In addition, 3096 special DEGs (sDEGs), with 2199 upregulated and 897 downregulated, were detected in the tolerant line L282, but not in the sensitive line L693. These sDEGs were primarily related to plasma membranes and oxygen-containing compounds. Furthermore, electric conductivity measurements demonstrated that the membrane of L282 experienced less damage, which is consistent with its strong tolerance at low temperatures. These results expand our understanding of the complex mechanisms involved in the cold germination of sweet corn and provide a set of candidate genes for further genetic analysis.

show abstract

“…Since cold positioning was first described in ferns [ 92 ], researchers have further elaborated on the links between photorelocation and cold acclimation in bryophytes [ 90 , 91 ] and flowering plants [ 90 ]. Phototropins act as thermosensors via blue-light regulated autophosphorylation [ 95 ] and subcellular re-localization [ 96–98 ], modulating the actin-dependent repositioning of chloroplasts as unfused aggregates in response to cold [ 99–101 ]. Seasonal rearrangement of chloroplasts was also observed in the mesophyll of two conifer species, in which chloroplasts move from the periphery along the cell wall in the summer to a more internal location in the cell in the winter ( Figure 1B ), a process that involves the vacuole and cytoplasmic strands [ 102 ].…”

Section: Chloroplastsmentioning

confidence: 99%

Visualizing the dynamics of plant energy organelles

Koenig,

Liu,

2023

Biochemical Society Transactions

View full text Add to dashboard Cite

Plant organelles predominantly rely on the actin cytoskeleton and the myosin motors for long-distance trafficking, while using microtubules and the kinesin motors mostly for short-range movement. The distribution and motility of organelles in the plant cell are fundamentally important to robust plant growth and defense. Chloroplasts, mitochondria, and peroxisomes are essential organelles in plants that function independently and coordinately during energy metabolism and other key metabolic processes. In response to developmental and environmental stimuli, these energy organelles modulate their metabolism, morphology, abundance, distribution and motility in the cell to meet the need of the plant. Consistent with their metabolic links in processes like photorespiration and fatty acid mobilization is the frequently observed inter-organellar physical interaction, sometimes through organelle membranous protrusions. The development of various organelle-specific fluorescent protein tags has allowed the simultaneous visualization of organelle movement in living plant cells by confocal microscopy. These energy organelles display an array of morphology and movement patterns and redistribute within the cell in response to changes such as varying light conditions, temperature fluctuations, ROS-inducible treatments, and during pollen tube development and immune response, independently or in association with one another. Although there are more reports on the mechanism of chloroplast movement than that of peroxisomes and mitochondria, our knowledge of how and why these three energy organelles move and distribute in the plant cell is still scarce at the functional and mechanistic level. It is critical to identify factors that control organelle motility coupled with plant growth, development, and stress response.

show abstract

The localization of phototropin to the plasma membrane defines a cold-sensing compartment in Marchantia polymorpha

Cited by 7 publications

References 46 publications

Functional comparison of phototropin from the liverworts Apopellia endiviifolia and Marchantia polymorpha

Functional comparison of phototropin from the liverworts Apopellia endiviifolia and Marchantia polymorpha

Transcriptome Analysis Identifies Novel Genes Associated with Low-Temperature Seed Germination in Sweet Corn

Visualizing the dynamics of plant energy organelles

Contact Info

Product

Resources

About