The localization of actin mRNA in megakaryocytes demonstrated by in situ hybridization using digoxigenin-labeled riboprobes.

Non-radiolabeled in situ hybridization and immunohistochemistry of .ALPHA.-smooth muscle actin in myoepithelial cells.

“…Recently digoxigenin-labeled probes (1) have been developed, which have some advantages, such as low background levels and ease of use. To detect labeled digoxigenin, we developed a combination of the PAP and ABC methods, with which the reactivity was enhanced in comparison with singlebridged immunodetection system (13).…”

Multiple-Labeling of Oligonucleotide Probes for In Situ Hybridization.

“…Previously [5,6], we utilized 35S-cRNA probes, which are thought to be the most sensitive [8]. However, the preparation of the probes often requires considerable expertise with molecular biological techniques such as cDNA cloning.…”