1998
DOI: 10.1267/ahc.31.275
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Multiple-Labeling of Oligonucleotide Probes for In Situ Hybridization.

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Cited by 5 publications
(4 citation statements)
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“…To prepare the antigen, we used synthetic complementary oligodeoxyribonucleotides designed to produce a double-stranded DNA flanked by the restric­tion enzyme sites, which was then ligated with the bacterial expression vector to produce recombinant oligopeptides. Similar use of oligodeoxyribonucleotides was introduced by Sasaki et al [12] to produce labeled ribonucleotide probes for in situ hybridization. The recombinant oligopeptide adopted in the present method possesses advantages of both the recombinant full-length protein and the synthetic oligo­peptide.…”
Section: Discussionmentioning
confidence: 99%
“…To prepare the antigen, we used synthetic complementary oligodeoxyribonucleotides designed to produce a double-stranded DNA flanked by the restric­tion enzyme sites, which was then ligated with the bacterial expression vector to produce recombinant oligopeptides. Similar use of oligodeoxyribonucleotides was introduced by Sasaki et al [12] to produce labeled ribonucleotide probes for in situ hybridization. The recombinant oligopeptide adopted in the present method possesses advantages of both the recombinant full-length protein and the synthetic oligo­peptide.…”
Section: Discussionmentioning
confidence: 99%
“…Because they could be readily obtained from a com- mercial source, we chose to conduct this study using oligonucleotide probes: earlier studies had shown that ISH could be successfully carried out using such oligonucleotide probes [6,11,14]. The sequence data of selected genes were obtained from the GenBank internet site, and the sequences of the probes are designed using commercially available computer software.…”
Section: Discussionmentioning
confidence: 99%
“…The testes were then perfused with Bouin’s fixative and immersed in the same fixative for more than 6 hr, dehydrated in ethanol, substituted with xylene, and embedded in paraffin. Paraffin sections (5 µm thick) were mounted on silane-coated slides [31]. Serial sections were mounted on several sets of three slides.…”
Section: Methodsmentioning
confidence: 99%