The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity

Abstract

A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35–90 % when microplates were conditioned before the addition of cells. A 80–90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20–60 % after incubation with biosurfactants while adhesion decreased by 80–90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10–40 kDa, which is one possible mechanism of action of the tested lipopeptides.Electronic supplementary materialThe online version of this article (doi:10.1007/s10482-015-0486-3) contains supplementary material, which is available to authorized users.

Lipid composition and cell surface hydrophobicity of Candida albicans influence the efficacy of fluconazole–gentamicin treatment

“…Under control conditions (Figure 1b, trial: C), the CAF4-2 strain was over threefold more hydrophobic than CAF2-1, which is similar to what was shown in our previous studies (Biniarz et al, 2015).…”

Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria

“…This compound significantly decreased the adhesion of tested bacteria and yeast to abiotic surfaces and had lower activity in a biofilm dislodging process (Janek et al, 2012). A similar effect was demonstrated by Biniarz et al (2015), who studied the pseudofactin and surfactin activity on Candida albicans. The authors observed a decrease in the adhesion of all tested C. albicans strains, and determined the synergistic interactions between the two tested lipopeptides.…”

“…The adhe- *++ zone with full transparency; + zone with incomplete transparency; +/-zone with weak transparency; -lack of transparency sion of 5 strains was decreased in the presence of surfactin at the concentrations of 0.1 and 0.2 mg × ml -1 , by 68.94±8.63% or 76.86±5.76%, respectively, whereas the presence of 0.4 mg × ml -1 caused a 81.02±5.22% reduction in the adhesion of only 3 strains. Biniarz et al (2015) found that pre-treatment of a polystyrene plate with surfactin was more effective when the concentrations of this compound were higher than critical micelle concentration (0.1 mg × ml -1 ). A concentrationdependent effect was also observed for pseudofactin II -the higher the concentration was used, the greater influence of the biosurfactant on microbial adhesion was observed (Janek et al, 2012).…”

Bacillomycin D and its combination with amphotericin B: promising antifungal compounds with powerful antibiofilm activity and wound-healing potency

“…With the aim to prevent hyphal development and infection, many researchers have also addressed the potential of antifungal compounds to inhibit germ tube formation when used at subinhibitory concentrations (Hammer et al 2000;Vale-Silva et al 2007;Naicker and Patel 2013). Lipopeptide compounds produced by Bacillus species have gained great attention in recent years as anti-Candida agents (Tabbene et al 2011;Rautela et al 2014;Biniarz et al 2015) and have revealed promising properties for the development of new compounds against a wide variety of fungi (Kim et al 2010;Ghribi and Chaabouni 2011;Xu et al 2013;Meena and Kanwar 2015). Among lipopeptides, bacillomycin D belonging to the iturin family is an heptapeptide linked to a fatty acid chain with different lengths (from 14 to 17 carbon atoms) (Peypoux et al 1984;Koumoutsi et al 2004;Tanaka et al 2014).…”