The lipid bilayer concept and its experimental realization: from soap bubbles, kitchen sink, to bilayer lipid membranes

Tien, H. Ti; Ottova, Angelica

doi:10.1016/s0376-7388(01)00394-5

Cited by 138 publications

(82 citation statements)

References 134 publications

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“…Membrane proteins constitute the largest fraction by weight of most cellular membranes (Tien and Ottova, 2001). They play a pivotal role in many cellular processes, for example, energy transfer, cell homeostasis, signal transduction and cell communication, and hence are highly relevant to human physiology and disease.…”

Section: Introductionmentioning

confidence: 99%

AFM study of the interaction of cytochrome P450 2C9 with phospholipid bilayers

Nussio

Voelcker

Miners

et al. 2010

Chemistry and Physics of Lipids

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Section: Introductionmentioning

confidence: 99%

AFM study of the interaction of cytochrome P450 2C9 with phospholipid bilayers

Nussio

Voelcker

Miners

et al. 2010

Chemistry and Physics of Lipids

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“…In light of these successful attempts an important question is raised: could the DIB respond to mechanical stimulus when appropriate biomolecules are incorporated? The interfacial forces acting on a DIB differ from those in other bilayer system 15,16 . Therefore, the tension in the bilayer held by the droplets could be controlled by regulating tension at the water-lipidoil interfaces; a concept not applicable with the painted or folded bilayer systems.…”

Section: Introductionmentioning

confidence: 87%

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Najem

Dunlap

Yasmann

et al. 2015

JoVE

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MscL, a large conductance mechanosensitive channel (MSC), is a ubiquitous osmolyte release valve that helps bacteria survive abrupt hypoosmotic shocks. It has been discovered and rigorously studied using the patch-clamp technique for almost three decades. Its basic role of translating tension applied to the cell membrane into permeability response makes it a strong candidate to function as a mechanoelectrical transducer in artificial membrane-based biomolecular devices. Serving as building blocks to such devices, droplet interface bilayers (DIBs) can be used as a new platform for the incorporation and stimulation of MscL channels. Here, we describe a micropipette-based method to form DIBs and measure the activity of the incorporated MscL channels. This method consists of lipid-encased aqueous droplets anchored to the tips of two opposing (coaxially positioned) borosilicate glass micropipettes. When droplets are brought into contact, a lipid bilayer interface is formed. This technique offers control over the chemical composition and the size of each droplet, as well as the dimensions of the bilayer interface. Having one of the micropipettes attached to a harmonic piezoelectric actuator provides the ability to deliver a desired oscillatory stimulus. Through analysis of the shapes of the droplets during deformation, the tension created at the interface can be estimated. Using this technique, the first activity of MscL channels in a DIB system is reported. Besides MS channels, activities of other types of channels can be studied using this method, proving the multi-functionality of this platform. The method presented here enables the measurement of fundamental membrane properties, provides a greater control over the formation of symmetric and asymmetric membranes, and is an alternative way to stimulate and study mechanosensitive channels.

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“…In the last years, several critical reviews have introduced changes in the proposal of Singer and Nicholson by including lipid mixtures, lipid heterogeneities, rafts, local curvatures and protein-lipid interactions (Israelachvili 1977;Ti Tien and Ottova 2001;Bagatolli et al 2010;Goñi 2014). However, in spite of several analyses and evidence available in relation to membrane hydration (Jendrasiak and Hasty 1974;Jendradiak et al 1996;Israelachvili and Wennerström 1996) the principal feature of the classical view still remained: water is not taken into account as a functional component.…”

Section: Introductionmentioning

confidence: 99%

Membrane Hydration: A Hint to a New Model for Biomembranes

Disalvo

2015

Subcellular Biochemistry

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The classical view of a biological membrane is based on the Singer-Nicholson mosaic fluid model in which the lipid bilayer is the structural backbone. Under this paradigm, many studies of biological processes such as, permeability, active transport, enzyme activity and adhesion and fusion processes have been rationalized considering the lipid membrane as a low dielectric slab of hydrocarbon chains with polar head groups exposed to water at each side in which oil/water partition prevails. In spite of several analyses and evidence available in relation to membrane hydration, water is not taken into account as a functional component. For this purpose, new insights in the water organization in restricted environments and the thermodynamical and mechanical properties emerging from them are specifically analysed and correlated.This chapter summarizes the progress of the studies of water in membranes along the book in order to give a more realistic structural and dynamical picture accounting for the membrane functional properties.

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The lipid bilayer concept and its experimental realization: from soap bubbles, kitchen sink, to bilayer lipid membranes

Cited by 138 publications

References 134 publications

AFM study of the interaction of cytochrome P450 2C9 with phospholipid bilayers

AFM study of the interaction of cytochrome P450 2C9 with phospholipid bilayers

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Membrane Hydration: A Hint to a New Model for Biomembranes

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