Membrane Hydration: A Hint to a New Model for Biomembranes

Disalvo, E.A.

doi:10.1007/978-3-319-19060-0_1

Cited by 26 publications

(32 citation statements)

References 62 publications

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“…In fact, in the absence of water, lipid membranes would not exist as their basic structural units self-assemble due to mainly hydrophobic effects. 8 …”

Section: Introductionmentioning

confidence: 99%

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Probing Residue-Specific Water–Protein Interactions in Oriented Lipid Membranes via Solid-State NMR Spectroscopy

et al. 2016

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Water plays a central role in membrane protein folding and function. It not only catalyzes lipid membrane self-assembly but also affects the structural integrity and conformational dynamics of membrane proteins. Magic angle spinning (MAS) solid-state NMR (ssNMR) is the technique of choice for measuring water accessibility of membrane proteins, providing a measure for membrane protein topology and insertion within lipid bilayers. However, the sensitivity and resolution of membrane protein samples for MAS experiments are often dictated by hydration levels, which affect the structural dynamics of membrane proteins. Oriented-sample ssNMR (OS-ssNMR) is a complementary technique to determine both structure and topology of membrane proteins in liquid crystalline bilayers. Recent advancements in OS-ssNMR involve the use of oriented bicellar phases that have improved both sensitivity and resolution. Importantly, for bicelle formation and orientation, lipid bilayers must be well organized and hydrated, resulting in the protein's topology being similar to that found in native membranes. Under these conditions, the NMR resonances become relatively narrow, enabling a better separation of 1H–15N dipolar couplings and anisotropic 15N chemical shifts with separated local field (SLF) experiments. Here, we report a residue-specific water accessibility experiment for a small membrane protein, sarcolipin (SLN), embedded in oriented lipid bicelles as probed by new water-edited SLF (WE-SLF) experiments. We show that SLN's residues belonging to the juxtamembrane region are more exposed to the water–lipid interface than the corresponding membrane-embedded residues. The information that can be obtained from the WE-SLF experiments can be interpreted using a simple theoretical model based on spin-diffusion theory and offers a complete characterization of membrane proteins in realistic membrane bilayer systems.

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“…In fact, in the absence of water, lipid membranes would not exist as their basic structural units self-assemble due to mainly hydrophobic effects. 8 …”

Section: Introductionmentioning

confidence: 99%

“…18 Nonetheless, the critical hydration level required for protein function may change from system to system, and low hydration levels are likely to affect both the structural dynamics and function of membrane-embedded enzymes. 8,19 …”

Section: Introductionmentioning

confidence: 99%

Probing Residue-Specific Water–Protein Interactions in Oriented Lipid Membranes via Solid-State NMR Spectroscopy

et al. 2016

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“…Instead, the thermodynamic aspects of the bilayer as a reactive surface considering its mechanical properties will specifically be discussed. This approach is based [37,38,[43][44][45]. In terms of interfacial properties, it is important to consider lipid interphases results that may give complementary information to present a more rigorous picture in terms of thermodynamic response.…”

Section: Stability and Membrane Response: A Dynamic Picturementioning

confidence: 99%

The Role of Water in the Responsive Properties in Lipid Interphase of Biomimetic Systems

Disalvo¹,

Frías²

2019

Liposomes - Advances and Perspectives

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The lack of details in the hydration properties of lipid bilayers hinders the design of biomimetic systems that, as liposomes and vesicles, may be used for biotechnological and medical purposes. In this chapter, studies indicate water as a membrane dynamic component determining the affinity and response of lipid membranes to amino acids, peptides and others stimuli. Based on thermodynamic analysis in lipid monolayers and its comparison with swelling shrinkage processes in liposomes and vesicles, it is concluded that: (1) the interphase of a lipid bilayer in a bidimensional solution of hydrated polar groups imbibed in labile water can be exchanged with the media by osmosis and or expansion-compression. (2) Excess water beyond the hydration shell (confined water) has solvent properties for additives in the bulk water phase and confers free energy that is in excess for binding of amino acids and peptides.(3) Dissolution in the water membrane phase changes the water activity (a w ) and affects the surface pressure. (4) Defects may be formed by the compression of bilayers in which carbonyl groups organized water differently. These studies indicate that a deeper understanding of the role of lipid bilayers in cellular biology and support the development of future lipid-based biotechnology that should necessarily include the role of water as a membrane dynamic component.

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“…[24] ïðèïóñòèëè, ùî aeîðñòê³ñòü ìåìáðàíè ìîaeå âïëèâàòè íà êîíôîðìàö³éíèé ñòàí PIP2;1, çñóâà-þ÷è ð³âíîâàãó â á³ê éîãî â³äêðèòî¿ êîíôîð-ìàö³¿, ùî é ñïðèÿº ïðîõîäaeåííþ âîäè. Òàêîae âèÿâëåíî, ùî ã³äðàòàö³ÿ ìåìáðàíè çá³ëüøóº ïðîñò³ð ì³ae àöèëüíèìè ëàíöþãàìè aeèðíèõ êèñëîò [34]. Òàêèì ÷èíîì, ÷åðåç çá³ëüøåíó ê³ëüê³ñòü ìîëåêóë âîäè íà ìåae³ ç'ºäíàííÿ ë³ï³ä/á³ëîê, óíàñë³äîê çá³ëüøåíî¿ íåíàñè÷åíîñò³ aeèðíèõ êèñëîò, ñïîñòåð³ãàëè çá³ëüøåííÿ ïðîíèêíîñò³ ÷åðåç àêâàïîðèíè [35].…”

Section: ô³ç³îëîã³ÿ ðîñëèíunclassified

Expression of aquaporin PIP2;1 as an indicator of Zea mays L. cultivar tolerance to reduced soil moisture

Shevchenko¹,

Ovrutska²,

Ovcharenko³

2019

Plant Varieties Studying and Protection

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Фізіологія рослин ізіологія рослин Ф Фізіологія рослин ізіологія рослин Âñòóï Äåô³öèò âîëîãè º îäíèì ç îñíîâíèõ àá³î-òè÷íèõ ñòðåñ³â, ùî âïëèâàº íà âðîaeàéí³ñòü, òîìó àêòóàëüíîñò³ íàáóâàº äîñë³äaeåííÿ ìå-õàí³çì³â àäàïòàö³éíèõ ìîaeëèâîñòåé ðîñëèí, çîêðåìà ñ³ëüñüêîãîñïîäàðñüêèõ êóëüòóð. Â³äîìî, ùî îäí³ºþ ç ïåðâèííèõ ì³øåíåé çîâ-í³øíüîãî ñòðåñó º öèòîïëàçìàòè÷íà ìåìáðàíà êë³òèí [1], ÿêà ðåàãóº íà íåñòà÷ó âîëîãè á³îõ³ì³÷íèìè ïåðåáóäîâàìè ë³ï³äíîãî á³øàðó òà çì³íîþ éîãî â'ÿçêîñò³. Ïëèííèé ñòàí öèòîïëàçìàòè÷íî¿ ìåìáðàíè òà àêòèâí³ñòü àêâàïîðèí³â çàáåçïå÷óþòü ñòàëå ôóíêö³îíóâàííÿ ìåìáðàíè â óìîâàõ íåñòà÷³ âîëîãè. Íåâåëèê³ ã³äðîôîáí³ á³ëêè àêâàïîðèíè (27-30 êÄà) ó ìåìáðàíàõ êë³òèí îðãàí³çîâàí³ ó âèãëÿä³ âèñîêîêîíñåðâàòèâíèõ òåòðàìåðíèõ ñòðóêòóð [2-4]. Àêâàïîðèíè ôîðìóþòü òðàíñìåìáðàíí³ êàíàëè, çàâäÿêè ÿêèì ì³ae êë³òèíàìè â³äáóâàºòüñÿ ïàñèâíèé òðàíñïîðò âîäè òà ðîç÷èíåíèõ ðå÷îâèí, ùî º îñíîâíèì âîäíèì øëÿõîì â óìîâàõ ïîñóõè [5]. Ó ãåíî-ì³ Zea mays L. âèÿâëåíî 36 ãåí³â, ÿê³ êîäóþòü àêâàïîðèíè [6]. Íàé÷èñåëüí³øà ðîäèíà àêâàïîðèí³â ðîñëèí -öå Ð²Ð-àêâàïîðèíè öèòîïëàçìàòè÷íî¿ ìåìáðàíè (plasma membrane intrinsic proteins), ÿêó ïîä³ëÿþòü íà äâ³ ãðóïè: Ð²Ð1 òà Ð²Ð2. Àêâàïîðèíè ãðóïè Ð²Ð2 åêñïðåñóºòüñÿ ïåðåâàaeíî â êîðåíÿõ ðîñëèí ³ õàðàêòåðèçóþòüñÿ á³ëüøîþ çäàò-í³ñòþ ïðîïóñêàòè âîäó ïîð³âíÿíî ç Ð²Ð1àêâàïîðèíàìè [5, 7]. Îñê³ëüêè Ð²Ð2-àêâàïîðèíè â³ä³ãðàþòü âàaeëèâó ðîëü ó âîäíîìó áàëàíñ³ êë³òèí, ð³âåíü åêñïðåñ³¿ ¿õí³õ ãåí³â ìîaeå çì³íþâàòèñÿ ï³ä ÷àñ çíåâîäíåííÿ ´ðóíòó. Â³äîìî, ùî äåô³öèò

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Membrane Hydration: A Hint to a New Model for Biomembranes

Cited by 26 publications

References 62 publications

Probing Residue-Specific Water–Protein Interactions in Oriented Lipid Membranes via Solid-State NMR Spectroscopy

Probing Residue-Specific Water–Protein Interactions in Oriented Lipid Membranes via Solid-State NMR Spectroscopy

The Role of Water in the Responsive Properties in Lipid Interphase of Biomimetic Systems

Expression of aquaporin PIP2;1 as an indicator of Zea mays L. cultivar tolerance to reduced soil moisture

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