The langmuir isotherm adsorption equation: The monolayer approach

Patiha,; Heraldy, Eddy; Hidayat, Yuniawan; Firdaus, Maulidan

doi:10.1088/1757-899x/107/1/012067

Cited by 67 publications

(29 citation statements)

References 4 publications

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“…3 ). For both oXiris and Toraymyxin, adsorption of LPS followed the Langmuir isotherm model [ 12 ]. Faster saturation of the LPS-adsorptive capacity was observed for oXiris than for Toraymyxin (Fig.…”

Section: Resultsmentioning

confidence: 99%

In vitro comparison of the adsorption of inflammatory mediators by blood purification devices

Malard

Lambert

Kellum

2018

ICMx

189

184

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BackgroundSeptic shock, a leading cause of acute kidney injury, induces release of pro-/anti-inflammatory mediators, leading to increased mortality and poor renal recovery. This is the first in vitro study directly comparing three single-use blood purification devices in terms of removing sepsis-associated mediators and endotoxins.MethodsIn vitro hemoperfusion was performed using oXiris®, CytoSorb®, and Toraymyxin®. Heparinized human plasma from healthy volunteers was pre-incubated with pathologic quantities of inflammatory mediators and filtered in a closed-loop circulation model for 2 h. For each device, the removal of 27 inflammatory mediators was measured over time. Endotoxin removal mediated by oXiris and Toraymyxin was assessed using hemoperfusion over 6 h.ResultsEndotoxin (lipopolysaccharide) removal was most rapid with Toraymyxin; mean adsorptive clearance over the first 30 min was ~ 20 ml/min vs ~ 8 ml/min with oXiris (p < 0.05). There was minimal endotoxin removal with CytoSorb (1 ml/min). At 120 min, there was no significant difference between the endotoxin removal rates using oXiris (mean ± standard deviation, 68.0 ± 4.4%) and Toraymyxin (83.4 ± 3.8%); both were significantly higher vs CytoSorb (− 6.3 ± 4.9%; p < 0.05). Total removal with oXiris was 6.9 μg vs 9.7 μg for Toraymyxin, where the total lipopolysaccharide quantity introduced was approximately 15.8 μg. Removal rates of pro-/anti-inflammatory cytokines and other inflammatory mediators were similar between oXiris and CytoSorb and were higher with CytoSorb and oXiris vs Toraymyxin. Granulocyte colony-stimulating factor was only effectively adsorbed by CytoSorb (99.4%). Differences were detected between the adsorption mechanism of the devices; binding to oXiris was mainly ionic, while CytoSorb was hydrophobic. No specific protein adsorption was found qualitatively with Toraymyxin.ConclusionsAdsorption rate kinetics varied for individual inflammatory mediators using the three blood purification devices. Mechanisms of adsorption differed between the devices. oXiris was the only device tested that showed both endotoxin and cytokine removal. oXiris showed similar endotoxin adsorption to Toraymyxin and similar adsorption to CytoSorb for the removal of other inflammatory mediators. Differences in device removal capacities could enable treatment to be more tailored to patients.Electronic supplementary materialThe online version of this article (10.1186/s40635-018-0177-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

In vitro comparison of the adsorption of inflammatory mediators by blood purification devices

Malard

Lambert

Kellum

2018

ICMx

189

184

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“…The Langmuir equation is strictly valid on a static volume from which adsorption on a membrane takes place and not on a fluid flow. In addition, the Langmuir mathematics assumes that the endotoxin-poly-imine ethylene layer is monolayer, which need not be the case on the oXiris membrane [7].…”

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confidence: 99%

Analysis of Endotoxin Adsorption in Two Swedish Patients with Septic Shock

Broman

Bodelsson

2019

Blood Purif

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Background: Lipopolysaccharide (endotoxin) from the outer Gram-negative bacterial wall can induce a harmful immunologic response, involving hemodynamic deprivation, and is one important motor driving the septic cascade. The positively charged poly-imine ethylene layer on the oXiris membrane is capable of adsorbing negatively charged endotoxin molecules and removing them from the blood compartment. Endotoxin is detrimental and should be removed from blood. Summary: The adsorbable endotoxin fraction in blood arises from a tight balance between seeding from an infectious focus and removal by an overwhelmed immune system. The net sum of remaining endotoxin in blood is available for an adsorption process in the oXiris filter. Endotoxin data from 2 patients with severe Gram-negative septic shock and endotoxemia in this case series, speaks for a considerable share of the adsorption of the oXiris filter in the endotoxin net removal over time. Key Messages: Analysis of combined in vitro and in vivo data speaks for an effect of the oXiris filter in lowering endotoxin.

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“…In case of adsorption, the bacterial cells become colored after the removal process. While in case of biodegradation, the bacterial cells maintain their original color 32 . In this study, no change in the color of bacterial cells was observed in the bacterial cells color after the decolorization process.…”

Section: Discussionmentioning

confidence: 99%

Novel Bacteria-Immobilized Cellulose Acetate/Poly(ethylene oxide) Nanofibrous Membrane for Wastewater Treatment

Zamel

Hassanin

Ellethy

et al. 2019

Sci Rep

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In this study, electrospun cellulose acetate - poly(ethylene oxide) nanofibrous membrane was found to be unique in immobilizing bacterial cells. Here, removal of methylene blue in aqueous media was achieved by using isolated species of bacteria (Bacillus paramycoides) from industrial wastewater and immobilized on cellulose acetate- poly(ethylene oxide) nanofibers using DMSO as a solvent. The decolorization time was varied from 0 to 72 h, different dye concentrations from 20 to 200 mg/L and bacterial cells count was investigated to achieve the maximum MB removal by bacteria-immobilized CA/PEO nanofibrous membrane. The effective dye decolorization was achieved within 48 h and MB removal % was around 93%. Furthermore, reusability of the bacteria-immobilized CA/PEO nanofibrous membrane was tested. It was found that after the 4th usage, 44% of the dye decolorization capacity still could be achieved. These results are promising and suggest that bacteria-immobilized CA/PEO nanofibrous membrane could be economically feasible and eco-friendly when used in MB removal from industrial wastewater. Combination of both adsorption and biodegradation methods was found to be effective in MB removal from aqueous media.

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The langmuir isotherm adsorption equation: The monolayer approach

Cited by 67 publications

References 4 publications

In vitro comparison of the adsorption of inflammatory mediators by blood purification devices

In vitro comparison of the adsorption of inflammatory mediators by blood purification devices

Analysis of Endotoxin Adsorption in Two Swedish Patients with Septic Shock

Novel Bacteria-Immobilized Cellulose Acetate/Poly(ethylene oxide) Nanofibrous Membrane for Wastewater Treatment

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