The Lambeth Conventions (II): Guidelines for the study of animal and human ventricular and supraventricular arrhythmias

Curtis, Michael J.; Hancox, Jules C.; Farkas, András; Wainwright, Cherry L.; Stables, Catherine L.; Saint, David A.; Clements‐Jewery, Hugh; Lambiase, Pier D.; Billman, George E.; Janse, Michiel J.; Pugsley, Michael K.; Ng, Guseva; Roden, Dan M.; Camm, A. John; Walker, Michael J.

doi:10.1016/j.pharmthera.2013.04.008

Cited by 252 publications

(188 citation statements)

References 280 publications

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“…In accordance with the Lambeth Conventions (27), arrhythmias were identified during the ischemia and reperfusion periods as ventricular fibrillation (VF), ventricular tachycardia (VT), and other types of arrhythmias (ventricular premature contraction (VPC)) including bigeminy, salvos and single extrasystoles ( Figure 1). A grade was given to each animal as an index of the severity of arrhythmias (arrhythmia score), according to the following scale: 0-no arrhythmia; 1-in the absence of VF, the duration of VT and/or VPC is shorter than 10 s or equal to 10 s; 2-in the absence of VF, the duration of VT and/or VPC is between 11 and 30 s; 3-in the absence of VF, the duration of VT and/or VPC is between 31 and 90 s; 4-the duration of reversible VF is shorter than 10 s or equal to 10 s and/or the duration of VT and/or VPC is between 91 and 180 s; 5-the duration of VF is longer than 10 s and/ or the duration of VT and/or VPC is longer than 180 s; and 6-irreversible VF (28).…”

Section: Recording and Arrhythmia Analysismentioning

confidence: 82%

Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Gonca¹

2015

Turk J Med Sci

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Background/aim: To investigate the influence of thiopental (85 mg/kg, intraperitoneally (ip)), and ketamine+xylazine (ketamine 75 mg/kg and xylazine 8 mg/kg, ip) anesthesia on the incidence and duration of ischemia/reperfusion-induced arrhythmias.Materials and methods: Myocardial ischemia was induced by a 6-min ligation of the left anterior descending coronary artery, followed by a 6-min reperfusion. Measurements were taken of the incidence and duration of ventricular arrhythmias, the mean arterial blood pressure and heart rate, and the pressure rate-product (as an index of myocardial oxygen consumption). Results:The arrhythmia score and the incidence of ventricular fibrillation and tachycardia were significantly decreased in the ketamine+xylazine-anesthetized rats compared with the thiopental-anesthetized group (arrhythmia score: 2.0 ± 2.1 versus 3.7 ± 1.2, P < 0.05). The heart rate was significantly lower in the ketamine+xylazine group during the entire experiment, whilst the pressure-rate product was also significantly lower in the ketamine+xylazine group at different time points of the ischemia and reperfusion periods. Conclusion:Ketamine+xylazine anesthesia has a strong antiarrhythmic effect and an apparent depressive action on the heart rate and the myocardial oxygen consumption index. Therefore, ketamine+xylazine anesthesia is not appropriate for the evaluation of possible antiarrhythmic agents. Thiopental anesthesia is preferable to ketamine+xylazine anesthesia in the in vivo ischemia-reperfusion arrhythmia model.

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Section: Recording and Arrhythmia Analysismentioning

confidence: 82%

Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Gonca¹

2015

Turk J Med Sci

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“…Arrhythmia types were diagnosed during the ischemic period in accordance with the Lambeth Conventions, [21] which are as follows: ventricular fibrillation (VF), ventricular tachycardia (VT), and ventricular premature contraction (VPC) including salvos, bigeminy and single extrasystoles (Figure 2). …”

Section: The Analyses Of Ecg and Blood Pressure Recordingsmentioning

confidence: 99%

Cardioprotective effect of zileuton: a 5-lipoxygenase inhibitor against myocardial ischemia/reperfusion injury

Gonca¹

2017

Turk Gogus Kalp Dama

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ÖZAmaç: Bu çalışmada zileuton'un miyokardiyal iskemi/ reperfüzyon hasarı ve iskemi ile uyarılan ventriküler aritmiler üzerine olan etkileri değerlendirildi ve 5-lipoksigenaz yolağının miyokardiyal iskemi/reperfüzyon hasarının patofizyolojisindeki rolü araştırıldı. Ça lış mapla nı:Anestezi altındaki sıçanlarda, miyokardiyal iskemi 120 dakikalık reperfüzyon periyodundan önce sol ana koroner arterin 30 dakikalık ligasyonu ile oluşturuldu. Zileuton 3 ve 10 mg/kg'lik dozlarda ligasyondan 15 dakika önce verildi. Deney süresince elektrokardiyografi, kan basıncı ve kalp atımı kayıt edildi. İskemik periyot boyunca aritmi tiplerinin süreleri tespit edildi. Miyokard dokusunda iskemi/reperfüzyon hasarını değerlendirmek için, histopatolojik inceleme yapıldı ve enfarkt alan 2,3,5-trifenil tetrazolyum klorit boyaması ile tespit edildi. Bul gu lar: Zileuton 3 mg/kg dozda enfarkt alan ve histopatolojik incelemeler sonucu elde edilen doku hasarı skorunda anlamlı bir azalmaya neden oldu (enfarkt alan [% risk alanı]: zileuton 3 mg/kg %36±7'ye kıyasla kontrol %66±6; p<0.05). 10 mg/kg zileuton etkili bulunmadı. Zileutonun 3 ve 10 mg/kg'lik dozu iskemi periyodunda ventriküler aritmilerin süresini kısaltmadı. So nuç: Çalışma sonuçlarımız zileutonun 3 mg/kg'lik dozda kalbi miyokardiyal iskemi/reperfüzyon hasarına karşı koruduğunu gösterdi. Ancak, zileuton iskemi ile uyarılan aritmileri azaltmada etkili değildi. Bu sonuçlara göre, zileuton miyokardiyal iskemi/reperfüzyon hasarının tedavisi için umut verici bir ilaç olabilir.Anah tarsöz cük ler: Miyokardiyal iskemi/reperfüzyon hasarı; sıçan modeli; ventriküler aritmi; zileuton. ABSTRACTBackground: This study aims to evaluate the effects of zileuton on myocardial ischemia/reperfusion injury and ischemia-induced ventricular arrhythmias and to investigate the role of 5-lipoxygenase pathway in the pathogenesis of myocardial ischemia/reperfusion injury. Methods: In anesthetized rats, myocardial ischemia was induced by the ligation of the left main coronary artery for 30 min before 120-min reperfusion period. Zileuton was given both at the doses of 3 and 10 mg/kg 15 min before the ligation. During the experiment, electrocardiography, blood pressure, and heart rate were recorded. The duration of arrhythmia types were determined during the ischemic period. To evaluate the ischemia/reperfusion injury in the myocardial tissue, histopathological examination was performed and the infarct size was determined by 2,3,5-triphenyltetrazolium chloride staining. Results:Zileuton at a dose of 3 mg/kg significantly decreased the infarct size and the tissue injury score obtained using histopathological examinations (infarct size [% of the area at risk]: zileuton 3 mg/kg 36±7% versus control 66±6%, p<0.05). Zileuton 10 mg/kg was found to be ineffective. Both 3 and 10 mg/kg doses of zileuton did not shorten the duration of arrhythmias during the ischemic period. Conclusion:Our study results showed that 3 mg/kg dose of zileuton protected the heart against myocardial ischemia/ reperfusion injury. However, it was i...

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“…Lead II electrocardiogram (ECG) was recorded by Bio Amp and monitored by a Power Lab system (ADInstruments, Australia) (Dianat et al 2014c).Ischemia-reperfusion induced ventricular arrhythmias were assessed according to the Lambeth conventions (Curtis et al 2013).Certain types of simple ventricular arrhythmia represent specific elaborations of the ventricular premature beats (VPB). Bigeminy has the minimum sequence VPB.…”

Section: Evaluation Of Ischemic-reperfusion Induced Ventricular Arrhymentioning

confidence: 99%

“…Sequence of a minimum of 4 consecutive ventricular complexes is defined as Ventricular tachycardia (VT). Ventricular fibrillation (VF) is defined as a sequence of a minimum of 4 consecutive ventricular complexes without intervening diastolic pauses, in which the intrinsic shape, the peak-peak interval and the height vary, and the variation between each is non-progressive (Curtis et al 2013). The criteria for subcategorization are: regularity of configuration (morphology); the rate (RR interval) and regularity of rate for VT, the duration of the arrhythmia, and the mode of termination for VT and VF.…”

Section: Evaluation Of Ischemic-reperfusion Induced Ventricular Arrhymentioning

confidence: 99%

The Effect of PM 10 on Ischemia- Reperfusion Induced Arrhythmias in Rats

Radmanesh

Dianat

Badavi

et al. 2016

Braz. arch. biol. technol.

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The Lambeth Conventions (II): Guidelines for the study of animal and human ventricular and supraventricular arrhythmias

Cited by 252 publications

References 280 publications

Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Cardioprotective effect of zileuton: a 5-lipoxygenase inhibitor against myocardial ischemia/reperfusion injury

The Effect of PM 10 on Ischemia- Reperfusion Induced Arrhythmias in Rats

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