Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Gonca, Ersöz

doi:10.3906/sag-1403-25

Cited by 13 publications

(9 citation statements)

References 35 publications

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“…Prior to in vivo electrophysiological studies, anaesthesia was induced using intramuscular injection of tiletamine/zolazepam (4-8 mg kg −1 ) only, to prevent anti-arrhythmic effects induced by xylazine (Gonca, 2015). Maintenance of anaesthesia and mechanical ventilation were performed as described above.…”

Section: In Vivo Electrophysiology Study and Map Recordingsmentioning

confidence: 99%

Altered adrenergic response in myocytes bordering a chronic myocardial infarction underlies in vivo triggered activity and repolarization instability

Dries

Amoni

Vandenberk

et al. 2020

The Journal of Physiology

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Ventricular arrhythmias are a major complication after myocardial infarction (MI), associated with sympathetic activation. The structurally heterogeneous peri-infarct zone is a known substrate, but the functional role of the myocytes is less well known. r Recordings of monophasic action potentials in vivo reveal that the peri-infarct zone is a source of delayed afterdepolarizations (DADs) and has a high beat-to-beat variability of repolarization (BVR) during adrenergic stimulation (isoproterenol, ISO). r Myocytes isolated from the peri-infarct region have more DADs and spontaneous action potentials, with spontaneous Ca 2+ release, under ISO. These myocytes also have reduced repolarization reserve and increased BVR. Other properties of post-MI remodelling are present in both peri-infarct and remote myocytes. r These data highlight the importance of altered myocyte adrenergic responses in the peri-infarct region as source and substrate of post-MI arrhythmias.

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Section: In Vivo Electrophysiology Study and Map Recordingsmentioning

confidence: 99%

Altered adrenergic response in myocytes bordering a chronic myocardial infarction underlies in vivo triggered activity and repolarization instability

Dries

Amoni

Vandenberk

et al. 2020

The Journal of Physiology

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“…Blood samples were collected from the jugular vein and sera were separated. Rats were euthanized using thiopental (85 mg/kg; i. p.; Gonca, 2015 ). The liver was divided into 2 parts; one part was kept in 10% neutral formalin, whereas the second part was stored in −80°C.…”

Section: Methodsmentioning

confidence: 99%

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

et al. 2021

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Tissue factor (TF) is a blood coagulation factor that has several roles in many non-coagulant pathways involved in different pathological conditions such as angiogenesis, inflammation and fibrogenesis. Coagulation and inflammation are crosslinked with liver fibrosis where protease-activated receptor1 (PAR1) and toll-like receptor4 (TLR4) play a key role. Antisense oligodeoxynucleotides are strong modulators of gene expression. In the present study, antisense TF oligodeoxynucleotides (TFAS) was evaluated in treating liver fibrosis via suppression of TF gene expression. Liver fibrosis was induced in rats by a single administration of N-diethyl nitrosamine (DEN, 200 mg/kg; i. p.) followed by carbon tetrachloride (CCl4, 3 ml/kg; s. c.) once weekly for 6 weeks. Following fibrosis induction, liver TF expression was significantly upregulated along with liver enzymes activities and liver histopathological deterioration. Alpha smooth muscle actin (α-SMA) and transforming growth factor-1beta (TGF-1β) expression, tumor necrosis factor-alpha (TNF-α) and hydroxyproline content and collagen deposition were significantly elevated in the liver. Blocking of TF expression by TFAS injection (2.8 mg/kg; s. c.) once weekly for 6 weeks significantly restored liver enzymes activities and improved histopathological features along with decreasing the elevated α-SMA, TGF-1β, TNF-α, hydroxyproline and collagen. Moreover, TFAS decreased the expression of both PAR1 and TLR4 that were induced by liver fibrosis. In conclusion, we reported that blockage of TF expression by TFAS improved inflammatory and fibrotic changes associated with CCl4+DEN intoxication. In addition, we explored the potential crosslink between the TF, PAR1 and TLR4 in liver fibrogenesis. These findings offer a platform on which recovery from liver fibrosis could be mediated through targeting TF expression.

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“…Due to its restricted therapeutic index, thiopental use should be limited to situations in which other anesthetics are not recommended (Tagawa and Sakuraba 2014;Gonca 2015). Accordingly, although previous anesthesia with thiopental preceding behavioral evaluation related to depression and anxiety had proved to be suitable, this anesthetic should be chosen with caution.…”

Section: Thiopental Effectsmentioning

confidence: 99%

Refinement of anesthetic choice in procedures preceding psychopharmacological studies

Gaigher

Siqueira

et al. 2018

Preprint

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RationalePrevious studies indicated that some general anesthetics induce long-term antidepressant and/or anxiolytic-like effects. This raises the concern about the use of anesthesia in surgeries that precede psycopharmacological tests, since it may be a potential bias on results depending on the experimental design used.ObjectivesTo evaluate whether commonly used general anesthetics in surgeries preceding psychopharmacological tests would affect rat behavior in tests predictive of antidepressant or anxiolytic-like effects. We also evaluated whether prior anesthesia would interfere in the detection of the antidepressant-like effect of imipramine or the anxiolytic-like effect of diazepam.MethodsWe tested if a single exposure to subanesthetic or anesthetic doses of 2,2,2-tribromoethanol, chloral hydrate, thiopental or isoflurane would change rat’s behavior in the forced swimming test (FST) or in the elevated plus-maze (EPM) test, at 2 hours or 7 days after administration.ResultsPrevious anesthesia with the aforementioned anesthetics did not change rat behavior in FST per se nor it changed the antidepressant-like effect induced by imipramine treatment. Rats previously anesthetized with tribromoethanol or chloral hydrate exhibited, respectively, anxiogenic-like or anxiolytic-like behavior in the EPM. Prior anesthesia with thiopental or isoflurane did not produce anyper seeffect in rat behavior in the EPM nor disturbed the anxiolytic-like effect of diazepam.ConclusionOur results suggest that, in our experimental conditions, tribromoethanol and chloral hydrate are improper anesthetics for surgeries that precede behavioral tests related to anxiety. Isoflurane or thiopental may be suitable for anesthesia before evaluation in animal models predictive of antidepressant or anxiolytic-like effect.

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Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Cited by 13 publications

References 35 publications

Altered adrenergic response in myocytes bordering a chronic myocardial infarction underlies in vivo triggered activity and repolarization instability

Altered adrenergic response in myocytes bordering a chronic myocardial infarction underlies in vivo triggered activity and repolarization instability

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

Refinement of anesthetic choice in procedures preceding psychopharmacological studies

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