The Lactic Acid Enterococci Enterococcus faecium and Enterococcus durans: Nucleotide Sequence Diversity in 16S rRNA Genes

Genome-based studies indicate that the Enterococcus faecium Clade B strains belong to Enterococcus lactis species and lack of the hospital infection associated markers

“…faecium [18]. However, differences in 16S rRNA gene sequences [19] and carbohydrate metabolism [20] have set these species apart. Morandi et al .…”

“…Clade A isolates carrying the phenotype for ampicillin resistance are described by PBP5-R sequences, while clade B genomes comprise susceptible strains displaying the PBP5-S profile [16] Enterococcus lactis, as its species name suggests, was firstly isolated from milk samples [17] and was recognized as a novel species closely related to E. faecium [18]. However, differences in 16S rRNA gene sequences [19] and carbohydrate metabolism [20] have set these species apart. Morandi et al [18] proposed E. lactis as a self-standing species of the genus Enterococcus, with strain BT159 T (=DSM 23655 T ,=LMG 25958 T ) as the type strain.…”

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

“…Secondly, 100 μL of each concentration was absorbed and coated on the surface of MRS AGAR medium, and it was placed in a 37 °C constant temperature incubator for 48 h. 30 After 48 h, the colony morphologies were observed, and suspected Lactobacillus colonies were picked out from the optimal dilution gradient MRS solid medium, inoculated in MRS liquid medium, and placed in a 37 °C constant temperature incubator for 24 h of culture. 31…”

“…Firstly, 1 g of each fermentation sample was accurately weighed into 9 mL of 0.85% sterile normal saline, and mixed evenly, and a 10 −3 -10 −6 sample diluent was prepared by a 10-fold stepwise dilute release method. 29 Secondly, 100 μL of each concentration was absorbed and coated on the surface of MRS AGAR medium, and it was placed in a 37 °C constant temperature incubator for 48 h. 30 After 48 h, the colony morphologies were observed, and suspected Lactobacillus colonies were picked out from the optimal dilution gradient MRS solid medium, inoculated in MRS liquid medium, and placed in a 37 °C constant temperature incubator for 24 h of culture. 31 In the third step, after the culture was completed, the morphologies of the bacteria were observed under an optical microscope by the Gram staining method.…”

Isolation, identification, and technological characterization of wild leuconostocs and lactococci for traditional Raib type milk fermentation

“…may belong to species Enterococcus lactis, as these isolates were genotypically related to E. faecium. This species, recently proposed for enterococci isolated from different dairy products (Bauer et al 2009;Morandi et al 2011a;Sukhodolets et al 2005), has been recently validated (Morandi et al 2011b).…”