The lack of genetic differentiation of pacu (Piaractus mesopotamicus) populations in the Upper-Paraguay Basin revealed by the mitochondrial DNA D-loop region: Implications for fishery management

Iervolino, Fabiana; Resende, E. K. de; Hilsdorf, Alexandre Wagner Silva

doi:10.1016/j.fishres.2009.09.003

Cited by 33 publications

(27 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Calcagnotto and DeSalle (2009) analyzed the genetic structure of P. mesopotamicus natural populations sampled inside and outside the Pantanal of Mato Grosso state, and did not observe a positive correlation between the genetic differentiation and the natural logarithm of geographical distances and when the sampling sites were compared there were also no genetic differentiation among them. Furthermore, Lervolino et al (2010), using the D-loop control region of mtDNA sequence data to investigate genetic variability within and among populations of P. mesopotamicus in the different rivers in the Upper-Paraguay Basin, observed the absence of genetic differentiation, suggesting that the populations could be treated as a single genetic stock. Differences could also be caused by the reproductive management in both hatchery stations, which could be the cause of the low genetic diversity in the broodstock A.…”

Section: Resultsmentioning

confidence: 99%

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Povh¹,

Ribeiro

Lopera-Barrero³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Monitoring the genetic diversity has fundamental importance for fish stocking programs. This experiment aims to evaluate the genetic diversity in two hatchery stations (A and B) with pacu Piaractus mesopotamicus (Holmberg, 1887) in Andirá, state of Paraná, Brazil used in stocking programs of Paranapanema River. Six microsatellite loci were amplified using DNA extracted from 60 fin-clipping samples. The broodstock B had the average number of alleles and the mean heterozygosity (alleles: 3.7 and H O : 0.628) higher than the broodstock A (alleles: 3.5 and H O : 0.600). Alleles with low frequency levels were observed in the both broodstocks. Evidence of linkage disequilibrium and lower allelic richness was found only in the broodstock A. Nei's gene diversity was high in both broodstocks. The genetic distance (0.085) and identity (0.918) showed similarity between broodstocks, which reflects a possible common origin. 6.05% of the total genetic variance was due to differences among broodstocks. Recent bottleneck effect in two broodstocks was observed. The results indicated a higher genetic diversity in the two broodstocks and they presented low genetic difference. This was caused by the reproductive management in both hatchery stations, reduction of population size and genetic exchange between the hatchery stations. Key words: Piaractus mesopotamicus, genetic variability, hatchery stations, molecular markers 6,05% da variância genética total foi devida a diferenças entre os estoques. Foi observado um recente efeito gargalo nos dois estoques. Os resultados indicaram uma alta diversidade genética nos estoques de reprodutores e baixa diferenciação genética entre eles, o que foi causado pelo manejo reprodutivo das pisciculturas, redução do tamanho populacional e intercâmbio genético entre as pisciculturas.

show abstract

Section: Resultsmentioning

confidence: 99%

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Povh¹,

Ribeiro

Lopera-Barrero³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

show abstract

“…The R ST values under the SMM are expected to be greater than the F ST values under the IAM (Slatkin, 1995), although the inverse has been observed in some fish species (e.g., Pereira et al, 2009). Low indexes of F ST have been observed in other studies with characiforms, such as in Colossoma macropomum (Santos et al, 2007), Piaractus mesopotamicus (Calcagnotto & DeSalle, 2009;Iervolino et al, 2010), Prochilodus argenteus (Hatanaka et al, 2006;Sanches et al, 2012), Prochilodus costatus (Carvalho-Costa et al, 2008), Prochilodus lineatus (Revaldaves et al, 1997) and Salminus brasiliensis (Lopes et al, 2007).…”

Section: Genetic Diversity and Lack Of Genetic Structuringmentioning

confidence: 91%

The roles of marginal lagoons in the maintenance of genetic diversity in the Brazilian migratory fishes Prochilodus argenteus and P. costatus

Melo

Sato²,

Foresti

et al. 2013

Neotrop. ichthyol.

View full text Add to dashboard Cite

The rio São Francisco basin contains many endemic species, such as Prochilodus argenteus and P. costatus, which have great commercial importance. However, information about the main recruitment sites and genetic studies containing extensive sampling of these species are scarce. To investigate the roles of the marginal lagoons in the maintenance of genetic variability and in the population structure, we analyzed six microsatellite loci in nine sampling groups of P. argenteus and five sampling groups of P. costatus. Our results showed high levels of genetic variability and low values of genetic differentiation for P. argenteus (F ST = 0.008, P < 0.05) and for P. costatus (F ST = 0.031, P < 0.05). In addition, high values of gene flow combined with a small genetic distance suggest the presence of a single population for each species in the middle rio São Francisco basin. Moreover, putative migration routes involving marginal lagoons during the reproductive season could be detected, confirming the importance of these nurseries in the lifecycle of these species. Our results also indicate the necessity of adequate management of the fish resources and the conservation of the floodplains in the rio São Francisco basin.A bacia do rio São Francisco contém muitas espécies endêmicas, tais como Prochilodus argenteus e P. costatus, os quais têm grande importância comercial. Entretanto, informações sobre as principais áreas de recrutamento e estudos genéticos contendo uma extensa amostragem dessas espécies no rio São Francisco são escassas. Para investigar o papel das lagoas marginais na manutenção da variabilidade genética e na estruturação populacional dessas espécies, nós analisamos seis loci microssatélites em nove grupos amostrais de P. argenteus e cinco grupos amostrais de P. costatus. Nossos resultados revelaram altos níveis de variabilidade genética para ambas as espécies e valores baixos de diferenciação genética para P. argenteus (F ST = 0.008, P < 0.05) e P. costatus (F ST = 0.031, P < 0.05). Adicionalmente, valores altos de fluxo gênico combinados com a distância genética baixa sugerem a presença de uma única população para cada espécie no médio rio São Francisco. Possíveis rotas migratórias envolvendo lagoas marginais durante o período reprodutivo puderam ser detectadas, confirmando a importância das lagoas marginais no ciclo de vida dessas espécies. Nossos resultados também indicaram a necessidade de um manejo adequado dos recursos pesqueiros e a conservação das várzeas na bacia do rio São Francisco.

show abstract

Section: Diversity and Biology Of Neotropical Fishmentioning

confidence: 99%

“…Neotropical fish may also be responsible for the proliferation of plant species due to their migratory behaviour. The pacu (P. mesopotamicus) is a migratory and omnivorous species that travels up to 300 km [42] and plays an important role as a seed disperser for many fleshyfruited plants of the Pantanal, contributing to seed dispersion for 27% of the tree community existing in the gallery forests [43].In addition to large migratory species that require long stretches of river and seasonal stimuli to exercise their life strategies, there are also sedentary species that carry out their vital activities in a restricted area and are more influenced by local environmental variations. Due to their small size, they spend their lives associated to a substrate, such as trunks, rocks and aquatic plants where they find protection, food and a suitable surface for egg deposition.…”

mentioning

confidence: 99%

“…D-loop regions were used to infer structural analyses of populations of pacu (P. mesopotamicus) from the Paraguay River and in four other main tributaries, showing high gene flow and the formation of a single panmictic population. This is due to the flood cycle that regulates the interconnectivity between different environments and allows gene flow between populations, forming metapopulations [42].There are few studies that use SNPs for the conservation of Neotropical freshwater species, and there are insufficient data to evaluate the genetic structure of natural stocks. More genetic studies using SNP markers for species identification need to be conducted in order to better understand population structure and to develop management measures and conservation policies [172,173].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Genetic Applications in the Conservation of Neotropical Freshwater Fish

Mastrochirico-Filho¹,

Freitas²,

Ariede³

et al. 2018

Biological Resources of Water

View full text Add to dashboard Cite

Neotropical fish correspond to approximately 30% of all fish species worldwide. The diversity of fish species found in Neotropical basins reflects variations in life-history strategies and exhibition of particular morphological, physiological and ecological attributes. These attributes are mainly related to different forms of feeding, life maintenance and reproduction. Today, fish populations are being threatened by anthropogenic actions that are having a visible impact on the natural state of continental aquatic ecosystems. The main causes are overfishing, non-native species introduction, reservoir-dam systems, mining, pollution and deforestation. The biology and population dynamics of the species are still unclear due to lack of research. Genetic tools can be useful resources for the conservation of Neotropical fish species in several ways. Molecular genetic markers are considered powerful tools to identify cryptic and hybrid fish and also allow the evaluation of the genetic variability and structure of populations of Neotropical ichthyofauna. Several analyses of molecular markers have been performed on Neotropical fish, including allozyme analysis, restriction fragment length polymorphisms in regions of DNA (RFLP), randomly amplified polymorphic DNA (AFLP), randomly amplified polymorphic DNA (RAPD), microsatellites, single nucleotide polymorphisms (SNPs) and mitochondrial DNA (mtDNA) markers. In order to analyse a high number of markers, next generation sequencing has allowed researchers to generate a large amount of genomic information that can be applied to the conservation of Neotropical fish.Keywords: molecular markers, genetic conservation, Neotropical ichthyofauna, overfishing, dam © 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Rivers of the Neotropical regionThe distribution of freshwater fish around the world was mediated by historical climatic and geological events at different time points. Today, each global region has distinct patterns of distribution due to physical barriers obstructing species dispersion, representing different tolerances to environmental variables [1]. The tropics of the American continent are well known for their high biodiversity. This is due to habitat heterogeneity and a complex geological history. The Neotropical region is a biogeographic region that comprises Central America (including the southern part of Mexico and the peninsula of Baja California), the south of Florida, the Caribbean and South America. The origin and evolution of the Neotropical region arose through a process of synergism between its fauna that experienced local rainfall variations and gradual climate change resulting in a mosaic of habitats controlled by river migrations, sea-level fluctuations, local dryness and local uplifts [2,3].Regional geo...

show abstract

The lack of genetic differentiation of pacu (Piaractus mesopotamicus) populations in the Upper-Paraguay Basin revealed by the mitochondrial DNA D-loop region: Implications for fishery management

Cited by 33 publications

References 31 publications

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

The roles of marginal lagoons in the maintenance of genetic diversity in the Brazilian migratory fishes Prochilodus argenteus and P. costatus

Genetic Applications in the Conservation of Neotropical Freshwater Fish

Contact Info

Product

Resources

About