The kinetics of glucose production from rice straw by Aspergillus niger

Aderemi, B.O.; Abu, E A; Highina, B. K.

doi:10.5897/ajb08.109

Cited by 13 publications

(4 citation statements)

References 20 publications

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“…Also, the physical treatment increases the surface area of the biomass, ensuring enzymatic hydrolysis. The particle size was kept around 2 mm which is the standard biomass size used to carry out enzymatic hydrolysis [23].…”

Section: Discussionmentioning

confidence: 99%

A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes

2018

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The present work refers to a process involving the use of dilute nitric acid pretreatment and enzymatic hydrolysis for the transformation of rice straw into simple sugars. Acid pre-treated rice straw was separated into the pulp and supernatant through centrifugation and filtration. The two fractions are then converted into simple sugars by combined action of microbes producing cellulase and laccase enzymes. These microbes were isolated from soil samples which were collected from different locations with varying altitudes, expected to harbour microbes with high-hydrolysing activity. The nitric acid pretreatment was carried out at 30 °C, 200 rpm for 72 h. After 72 h, the culture supernatants were analysed for the presence of glucose with the help of HPLC. The supernatant fraction separated after the acid pre-treated rice straw produced highest amount of glucose (205 mg/g of rice straw) upon subsequent hydrolysis with synergistic action of cellulase and laccase-producing microbes.

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Section: Discussionmentioning

confidence: 99%

A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes

2018

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“…The lag phase of A. brasiliensis has been shown to be within the range of 12 to 24 h in a previous study, where it was cultivated in media containing glucose (Ho and Hood, 2014). Similar lag phase times (5 to 10 h) has been associated with the commonly used and closely related A. niger for CA production (Baei et al, 2008). The exponential phase or logarithmic phase transpires when the microbial culture undergoes asexual reproduction at a constant rate through simple cell division, in which one cell separates forming two identical daughter cells after undergoing nuclear division (Ellena et al, 2020, Asaduzzaman, 2007.…”

Section: A Brasiliensis Growth Kinetics For the Ssfoptimizedfw Ssfdww...mentioning

confidence: 53%

“…The maximum (Xmax) and initial (X0) biomass cell concentrations are essential indicators for the synthesis of biomass and formation of products (Baei et al, 2008)…”

Section: A Brasiliensis Growth Kinetics For the Ssfoptimizedfw Ssfdww...mentioning

confidence: 99%

“…The enzymes secreted during growth are related to the lignocellulosic substrate, while temperature fluctuations influence extracellular enzyme activity(Zhang et al, 2018). study andBaei et al (2008) may be credited to several aspects such as substrate type, microbial strain, nutrient availability, heat and mass transfer, aeration, agitation, pH, solid and enzyme loading.…”

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Development of innovative pretreatments for simultaneous saccharification and citric acid production from banana pseudostem: bioprocess optimization and kinetic assessment.

Laltha

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The bioconversion of renewable lignocellulosic biomass into value-added products such as citric acid (CA) has become a research hotspot towards a sustainable economy. Approximately 2 million tons of CA are produced annually with widespread use in the food, chemical, pharmaceutical and textile industries. Nevertheless, the use of lignocellulosic-derived feedstocks for CA production requires expensive, energy- and resource intensive pretreatment and fermentation processes, that result in low product yields. Therefore, the present study explores the valorization of banana pseudostem (BP) for CA production using the simultaneous saccharification and fermentation (SSF) process to alleviate these challenges. A review on the potential of BP as a feedstock for microbial production of value-added products is discussed together with the pretreatment and bioprocess challenges in addition to the possibilities to overcome these bottlenecks. Subsequently, two novel pretreatments consisting of a: (1) microwave-assisted-iodized table salt (M-ITS) and (2) microwave-assistedpaper wastewater (M-PWW) were modelled and optimized using the response surface methodology (RSM) for the enhancement of sugar recovery from BP. Then, the SSF process with pretreated BP was modelled and optimized using RSM for CA production. Thereafter, the logistic and modified Gompertz models were used to assess the kinetics of Aspergillus brasiliensis growth and CA production, respectively. The pretreatment input parameters for the M-ITS model comprised of salt concentration (1- 5%, w/v), microwave power intensity (100-900 W) and pretreatment time (2-10 min). On the other hand, the M-PWW model consisted of substrate solid loading (10-30% w/v), microwave power intensity (100-900 W) and pretreatment time (2-10 min). The output responses were reducing sugar yield (RSY) and glucose yield (GY) for both models. The optimal pretreatment conditions predicted by the M-ITS model were 2.48% ITS concentration, 800 W (power intensity) and 10 minutes (pretreatment time), corresponding to an experimental RSY (0.515 g/g) and GY (0.433 g/g). Pretreatment and optimization revealed a slightly higher RSY and GY for the M-ITS strategy when compared to the M-PWW method (RSY = 0.498 g/g and GY = 0.413 g/g). The M-PWW model predicted optimal pretreatment conditions of 30% solid loading, 800 W for 8 minutes. Despite the higher sugar yields observed for the M-ITS pretreatment, the M-PWW pretreatment represents a more suitable strategy owing to its complete waste-based approach that generates three times the quantity of pretreated substrate required for subsequent fermentation. SSF optimization included nitrogen (ammonium nitrate) concentration (0.5-5 w/v), desorbent (acetone) concentration (1-5% v/v) and temperature (30-40 °C) as inputs with CA concentration as the output (g/L). The optimized SSF conditions (0.5% w/v ammonium nitrate, 1% v/v acetone and 35 °C) (SSFoptimizedFW) gave a maximum CA concentration of 14.408 g/L. For the kinetic experiments, three bioprocesses consisting of the: (1) optimized SSF process with freshwater (SSFoptimizedFW), (2) SSFoptimizedFW process conditions while substituting dairy wastewater in place of freshwater (SSFDWW), and (3) Sabouraud Dextrose Emmon’s medium modified by substituting glucose with BP (SSFSDEmodified), were assessed. While all three bioprocesses gave the same maximum specific growth rate (μmax) of 0.05 h-1, the SSFSDEmodified process resulted in the highest maximum potential CA concentration (Pm) (13.991 g/L) compared to the SSFDWW (Pm= 13.095 g/L) and SSFoptimizedFW (Pm= 12.967 g/L) systems. The developed pretreatment strategies demonstrated glucose yields >0.40 g/g, shown to be higher than previously established chemical pretreatments. Moreover, the SSFDWW process displayed a slightly lower Pm value compared to the SSFSDEmodified strategy, and interestingly a higher Pm value than the SSFoptimizedFW bioprocess. Major findings from this study paves the way for lignocellulosic bioprocesses by potentially negating the use of costly pretreatment chemicals, fermentation medium constituents and/or fresh water, while achieving a “waste treating waste” approach. Thus, contributing to the water-energy-food nexus in line with global sustainable development goals towards a circular bioeconomy.

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Using Pretreatment and Enzymatic Saccharification Technologies to Produce Fermentable Sugars from Agricultural Wastes

Huang

Jeuck

Yong

2017

Waste Biomass Management – A Holistic Approach

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The kinetics of glucose production from rice straw by Aspergillus niger

Cited by 13 publications

References 20 publications

A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes

A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes

Development of innovative pretreatments for simultaneous saccharification and citric acid production from banana pseudostem: bioprocess optimization and kinetic assessment.

Using Pretreatment and Enzymatic Saccharification Technologies to Produce Fermentable Sugars from Agricultural Wastes

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