The kinetics of 1,4‐butanediol diglycidyl ether crosslinking of dermal sheep collagen

Zeeman, Raymond; Dijkstra, Pieter J.; Wachem, P.B. van; Luyn, Marja J. A. van; Hendriks, Marc; Cahalan, Patrick T.; Feijén, Jan

doi:10.1002/1097-4636(20000915)51:4<541::aid-jbm1>3.3.co;2-g

Cited by 6 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This scaffold, loaded with differentiated cells (e.g., articular chondrocytes) or bone marrow stromal cells, has been shown to support cartilage and bone tissue formation selectively in ectopic models 20. The scaffold was chemically crosslinked through a biocompatible organic reticulation agent21 to provide stability, thus increasing in situ hydrophilic properties and good handling properties, including flexibility. The purpose of this experimental study was to evaluate the safety and regenerative potential of the newly developed scaffold in osteochondral defects in a sheep model.…”

mentioning

confidence: 99%

Orderly osteochondral regeneration in a sheep model using a novel nano‐composite multilayered biomaterial

Kon

Delcogliano

Filardo

et al. 2009

Journal Orthopaedic Research

163

158

View full text Add to dashboard Cite

The objective of this article was to investigate the safety and regenerative potential of a newly developed biomimetic scaffold when applied to osteochondral defects in an animal model. A new multilayer gradient nano-composite scaffold was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. In the femoral condyles of 12 sheep, 24 osteochondral lesions were created. Animals were randomized into three treatment groups: scaffold alone, scaffold colonized in vitro with autologous chondrocytes and empty defects. Six months after surgery, the animals were sacrificed and the lesions were histologically evaluated. Histologic and gross evaluation of specimens showed good integration of the chondral surface in all groups except for the control group. Significantly better bone regeneration was observed both in the group receiving the scaffold alone and in the group with scaffold loaded with autologous chondrocytes. No difference in cartilage surface reconstruction and osteochondral defect filling was noted between cell-seeded and cell-free groups. In the control group, no bone or cartilage defect healing occurred, and the defects were filled with fibrous tissue. Quantitative macroscopic and histological score evaluations confirmed the qualitative trends observed. The results of the present study showed that this novel osteochondral scaffold is safe and easy to use, and may represent a suitable matrix to direct and coordinate the process of bone and hyaline-like cartilage regeneration. The comparable regeneration process observed with or without autologous chondrocytes suggests that the main mode of action of the scaffold is based on the recruitment of local cells. ß

show abstract

mentioning

confidence: 99%

Orderly osteochondral regeneration in a sheep model using a novel nano‐composite multilayered biomaterial

Kon

Delcogliano

Filardo

et al. 2009

Journal Orthopaedic Research

163

158

View full text Add to dashboard Cite

show abstract

“…The reaction between BDDGE and amine groups of lysine and hydroxylysine occurs via two distinct steps: first, the formation of pendant epoxide groups in the collagenous matrix takes place and then, the evolution to bridges between the protein chains . The ratio between pendant epoxide groups and crosslinks is governed by pH, concentration of reactants, and temperature …”

Section: Resultsmentioning

confidence: 99%

“…Crosslinking with multifunctional epoxy reagents is a less explored but promising alternative since in vitro studies have shown that cytotoxicity of epoxide compounds is within acceptable levels . Moreover, chemical reactions between collagen and epoxy compounds in basic media generate stable protein chain crosslinking, yielding materials with mechanical properties comparable to those achieved with glutaraldehyde …”

Section: Introductionmentioning

confidence: 99%

Mechanical behavior of cold‐water fish gelatin gels crosslinked with 1,4‐butanediol diglycidyl ether

Czerner

Prudente

Martucci

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this work, chemical crosslinking with 1,4‐butanediol diglycidyl ether (BDDGE) is used as strategy to enhance mechanical performance of fish gelatin (FG) gels in order to meet the properties' range of mammalian gelatin physical gels. Joint analysis of free amino groups, swelling ratio, and total soluble material indicates that crosslinking degree increases with increasing FG concentration and it is favored by a 0.2 BDDGE/FG ratio. Increasing crosslinking degree enhances gel indentation strength and shear modulus (μ) while decreases fracture toughness (GIC). Measured μ and GIC values lies within the range exhibited by mammalian gelatin physical gels, but the relationship between these parameters is opposite. This is due to the different fracture mechanisms occurring in chemically crosslinked and physical gels.

show abstract

“…[1][2][3][4][5] . In recent years, the modification of gelatin through chemical reaction between amino groups and compounds containing epoxy groups has attracted more attention [6][7][8][9] . The modified gelatin can be applied as biomedical materials 10 .…”

Section: Introductionmentioning

confidence: 99%

Studies on the Reaction of Allyl Glycidyl Ether with Gelatin by Van Slyke Method

Li¹,

Tang²,

Yang³

et al. 2013

Asian J. Chem.

View full text Add to dashboard Cite

The kinetics of 1,4‐butanediol diglycidyl ether crosslinking of dermal sheep collagen

Cited by 6 publications

References 0 publications

Orderly osteochondral regeneration in a sheep model using a novel nano‐composite multilayered biomaterial

Orderly osteochondral regeneration in a sheep model using a novel nano‐composite multilayered biomaterial

Mechanical behavior of cold‐water fish gelatin gels crosslinked with 1,4‐butanediol diglycidyl ether

Studies on the Reaction of Allyl Glycidyl Ether with Gelatin by Van Slyke Method

Contact Info

Product

Resources

About