The Isotope Dilution Procedure of Analysis. I. Historical and Literature Survey

Pinajian, J.J.; Christian, John E.; Wright, Walter E.

doi:10.1002/jps.3030420509

Cited by 9 publications

(6 citation statements)

References 42 publications

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“…In this situation, many or most monitored proteins and small molecules are unanticipated at the time of laboratory study, thus eliminating the possibility of prior investigation of relative sensitivity factors (RSFs). Furthermore, methods based on introducing a known amount of a chemically analogous extraneous substance as an internal standard (i.e., “spiking” of a standard reference material) are not practical, whether the analogue is chemically identical and isotopically labeled − or based on chemical similarity. , …”

mentioning

confidence: 99%

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

Wang¹,

Zhou²,

Lin³

et al. 2003

Anal. Chem.

625

618

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A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization. This biotechnology provides differential expression measurements and enables the discovery of biological markers (biomarkers). Work presented here uses human serum but is applicable to any fluid or tissue. The approach relies on linearity of signal versus molecular concentration and reproducibility of sample processing. There is no use of isotopic labeling or chemically similar standard materials. Linear standard curves are reported for a variety of compounds introduced into human serum. As a measure of analytical reproducibility for proteome and metabolome sampling, median coefficients of variation of 25.7 and 23.8%, respectively, were determined for approximately 3400 molecular ions (not counting their numerous isotopes) from 25 independently processed human serum samples, corresponding to a total of 85000 individual molecular ion measurements.

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mentioning

confidence: 99%

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

Wang¹,

Zhou²,

Lin³

et al. 2003

Anal. Chem.

625

618

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“…The potential of stable isotope labeling has also been explored in connection with kinetic metabolic studies. The design, data evaluation and general information content of kinetic isotope dilution experiments has been described in detail (Shemin & Foster, ; Bloch & Anker, , for reviews see Pinajian, Christian, & Wright, ; Christian & Pinajian, ; Zilversmith, ). Also, a first‐hand detailed review summarized early metabolic studies, covering both radio‐ and stable isotopes (Hevesy, ).…”

Section: –1950 Stable Isotopes and Radioisotopes Of Light Bioelemmentioning

confidence: 99%

A timeline of stable isotopes and mass spectrometry in the life sciences

Lehmann

2016

Mass Spec Rev

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This review retraces the role of stable isotopes and mass spectrometry in the life sciences. The timeline is divided into four segments covering the years 1920-1950, 1950-1980, 1980-2000, and 2000 until today. For each period methodic progress and typical applications are discussed. Application of stable isotopes is driven by improvements of mass spectrometry, chromatography, and related fields in sensitivity, mass accuracy, structural specificity, complex sample handling ability, data output, and data evaluation. We currently experience the vision of omics-type analyses, that is, the comprehensive identification and quantification of a complete compound class within one or a few analytical runs. This development is driven by stable isotopes without competition by radioisotopes. In metabolic studies as classic field of isotopic tracer experiments, stable isotopes and radioisotopes were competing solutions, with stable isotopes as the long-term junior partner. Since the 1990s the number of metabolic studies with radioisotopes decreases, whereas stable isotope studies retain their slow but stable upward tendency. Unique fields of stable isotopes are metabolic tests in newborns, metabolic experiments in healthy controls, newborn screening for inborn errors, quantification of drugs and drug metabolites in doping control, natural isotope fractionation in geology, ecology, food authentication, or doping control, and more recently the field of quantitative omics-type analyses. There, cells or whole organisms are systematically labeled with stable isotopes to study proteomic differences or specific responses to stimuli or genetic manipulation. The duo of stable isotopes and mass spectrometry will probably continue to grow in the life sciences, since it delivers reference-quality quantitative data with molecular specificity, often combined with informative isotope effects. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:58-85, 2017.

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“…Pinajian and cow'orkers (429) have given an excellent review of the history of this method and list 78 references to the original literature in a survey of the field. Christian and Pinajian (79) describe three procedures of analysis-the direct isotope dilution analysis, the inverse isotope dilution analysis, and the double isotope dilution analysis-and discuss the limitations of each.…”

Section: Isotope Dilutionmentioning

confidence: 99%

Nucleonics

Meinke¹

1956

Anal. Chem.

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THISarticle reviews the publications in nucleonics from 1953 to the present time. It follows, without overlapping, the material presented in the previous review (167).During the past 2 years, the scope of the field has enlarged tremendously. In this country, the change in the Atomic Energy Act in 1954 caused a veritable explosion among industries as they sought to enter the atomic energy field. In Europe, the Atomic Energy Research Establishment at Harwell, England, as well as several other groups has continued to supply tracers in increasing quantities. Several general papers presented recently (1, 2, 4, 132,249,251,379,492) give excellent summaries of the important uses of radioisotopes in analysis in the United States, the United Kingdom, and Russia.In reviewing publications of such an extensive field, it is difficult to discuss every pertinent paper. It was felt, however, that this type of review should emphasize the widespread use of radioisotopes in analysis today. An effort has therefore been made to include many references illustrating the use of activation, isotope dilution radioassay, and radiotracer methods in analysis. It is hoped that from such a compilation the analyst will discover uses for radioisotopes in his own work, realizing that this technique is not one that is still in the "curiosity" stage, but rather one that has already "arrived."It was necessary to omit many worth-while papers because of limitations of time and space and the inaccessibility of certain journals. A number of papers dealing with techniques and measurements have also been omitted because they had alreadyappeared in extensive bibliographies of summary papers. Manysuch summarypapers, which were presented at the International Conference on the Peaceful Uses of Atomic Energy held at Geneva (399) in August 1955, are included in this review.The present paper gives the general references at the beginning of each section, followed by-specific references to items of more limited application. It has not been possible to cross index all the methods listed in the general papers. Therefore, under each major section, it is important that the general survey-papers as well as the specific individual papers be read for a complete picture of progress in the field.

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The Isotope Dilution Procedure of Analysis. I. Historical and Literature Survey

Cited by 9 publications

References 42 publications

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

A timeline of stable isotopes and mass spectrometry in the life sciences

Nucleonics

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