The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia

Abstract

In this study, the diseased fish samples were collected from fish farms in the nine different dam lakes during the summer months between July 2014 and September 2018. It is isolated twenty-two bacteria from diseased rainbow trout (Oncorhynchus mykiss) eyes showing exophthalmos symptoms. All isolates identification was carried out by using conventional biochemical tests, API 20 Strep test kit and PCR test. ATCC 43921 reference strain of the Lactococcus garvieae was used as a positive control. The PCR test was confirmed by using the pLG-1 and pLG-2 reference genes (16S rRNA) specific for the L. garvieae species. As a result of this study, all of the twenty-two isolates isolated from the eyes of diseased rainbow trout were confirmed as L. garvieae by PCR test. According to the results of antibiotic susceptibility test, all of the L. garvieae isolates were resistant to streptomycin, sulfamethoxazole, and sulfamethoxazole/trimethoprim. The same isolates have been determined to be resistant to ampicillin (94.2%), oxytetracycline (72.5%), erythromycin (55.07%), oxolinic acid (43.5%), enrofloxacin (26.09%), doxycycline (20.3%), amoxicillin and florfenicol (8,7%) as well. Thus, florfenicol, amoxicillin, doxycycline, and enrofloxacin were found as the most effective antibiotics.

A Mixed Frigoribacterium faeni and Lactococcus garvieae Infection in Cultured Rainbow Trout (O. mykiss)

“…Fish samples showed similar clinical external and internal symptoms such as darkening of the skin, hemorrhages, and splenomegaly as reported in previous lactococcosis cases (Kang et al, 2004;Altun et al, 2005;Vendrell et al, 2006;Özer et al, 2008;Avcı et al, 2010;Öztürk et al, 2013;Avcı et al, 2014;Didinen et al, 2014;Ürkü and Timur, 2014;Balta and Balta, 2019) with slight variations. As an expectation, similar symptoms in the eyes such as exophthalmos, hemorrhages and opacification of the cornea was observed in the fish samples but not the loss of eyes as reported by Timur et al, (2011) and Öztürk et al (2013).…”

Identification and Differences in Antimicrobial Susceptibility of <em>Lactococcus</em> <em>garvieae</em> from Farmed Grey Mullet (<em> Mugil cephalus </em>) and Non-Grey Mullet in Southern Taiwan

“…The diseased fishes in this study exhibited corneas with a turbid, whitened, or red appearance and especially those with severe infection exhibited budging eyes on one or both sides. These symptoms were similar to the finding on rainbow trout infected with L. garvieae infection [25]. [10].…”

Fırtına Deresindeki Gökkuşağı Alabalık Çiftliklerinde İzole Edilen Aeromonas spp. İzolatlarının Antimikrobiyel Hassasiyetin Belirlenmesi

“…Başka bir araştırmada, fenotipik karekterler ile Aeromonadların tanımlanamadığı için mikroplaka hibridizasyon yönteminin kullanılması ile daha doğru tanımlamanın yapılacağı bildirilmiştir (Sugita vd., 1994). Bu araştırmada, ampisilin ve amoksisilin'e karşı %100, sulfametoksazol %79,31, oksitetrasiklin %62,07, eritromisin %51,72, trimetoprim + sulfametoksazol kombinasyonuna karşı %48,27 direnç gelişmiş olması kaygı uyandırıcı olması yanında sucul ortamdaki diğer mikroorganizmalar ile antibiyotiklerin direkt veya metabolitlerinin teması ile direnç şekillenmesine neden olduğunu bildiren birçok yayın mevcuttur (Alzainy, 2011;Balta, 2016;Balta vd., 2016;Balta & Dengiz Balta, 2017;Balta & Dengiz Balta, 2019;Balta & Yılmaz, 2019;Samal vd., 2014). Gökkuşağı alabalık yavrularından izole edilen A. caviae (636, 706, 709, 710, 711, 712, 722, 727, 762, 769 ve 779) .…”