The isolation of enteroviruses from blood: A comparison of four processing methods

Prather, Sandra L.; Dagan, Ron; Jenista, Jerri Ann; Menegus, Marilyn A.

doi:10.1002/jmv.1890140305

Cited by 44 publications

(18 citation statements)

References 17 publications

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“…After enteroviruses replicate in the tissues of the respiratory and gastrointestinal tracts, they can be present in blood and spread to secondary target organs [4]. A viremia is often detected in hospitalized patients with enterovirus diseases [7]. We recently detected enteroviral RNA with a strong homology with CVB3 and CVB4 in the blood of patients with insulin-dependent type 1 diabetes.…”

Section: Igg Devoid Of Neutralizing Activity and Isolated From Donor mentioning

confidence: 98%

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Antibody‐Dependent Enhancement of Coxsackievirus B4 Infectivity of Human Peripheral Blood Mononuclear Cells Results in Increased Interferon‐α Synthesis

Hober¹,

Chehadeh

Bouzidi

et al. 2001

J INFECT DIS

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IgG devoid of neutralizing activity and isolated from donor plasma by chromatography formed immune complexes with coxsackievirus B4 (CVB4) and significantly increased the infection of peripheral blood mononuclear cells with CVB4. The major host cells for CVB4 infection enhanced with IgG are monocytic CD14+ cells. The roles of CVB and adenovirus receptor and Fcgamma receptor II and III have been shown. Increased viral replication and the release of infectious particles were demonstrated when interferon (IFN)-alpha produced by infected cells was first neutralized by use of antibodies. The CVB4 IgG-induced synthesis of IFN-alpha by monocytes reflected entry and uncoating of CVB4 but not of viral replication and required the presence of CVB4 RNA inside the cells. Thus, CVB4 can infect monocytes by an antibody-dependent mechanism through interactions between the virus, antiviral antibodies, and specific receptors that result in IFN-alpha production.

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Section: Igg Devoid Of Neutralizing Activity and Isolated From Donor mentioning

confidence: 98%

“…CVB can be recovered from mononuclear cells [7]. However, it is not clear whether the virus replicates within these cells.…”

Section: Igg Devoid Of Neutralizing Activity and Isolated From Donor mentioning

confidence: 99%

Antibody‐Dependent Enhancement of Coxsackievirus B4 Infectivity of Human Peripheral Blood Mononuclear Cells Results in Increased Interferon‐α Synthesis

Hober¹,

Chehadeh

Bouzidi

et al. 2001

J INFECT DIS

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“…This may be followed by the occurrence of infectious virus in blood and lead to spreading of the pathogens to secondary target organs. During this viraemic phase, enteroviruses have been cultured from the serum of patients (Dagan et al, 1985 ;Prather et al, 1984) indicating haematogenous spread of the viruses in free form. The finding that enteroviruses can also be isolated from blood cells of patients (Dagan et al, 1985 ;Prather et al, 1984) Author for correspondence : Tytti Vuorinen.…”

Section: Introductionmentioning

confidence: 99%

Enterovirus receptors and virus replication in human leukocytes.

Vuorinen¹,

Vainionp²,

Heino

et al. 1999

Journal of General Virology

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Although enteroviruses cause a great variety of diseases including meningitis, paralysis and myocarditis, the life-cycle of these viruses in man is still quite poorly understood. The role of human leukocytes as a target for enterovirus infections was studied in this report. Despite great similarity in the structure and replication of coxsackievirus B3 (CBV3), echovirus 1 (EV1), and poliovirus 1 (PV1), the ability of these viruses to infect human peripheral blood mononuclear cells (PBMC), and B (Raji), T (Molt-4) and monocytic (U-937) cell lines differed markedly. CBV3 attached both to PBMC and the three haematopoietic cell lines studied, whereas EV1 bound only to PBMC. Generally, the attachment of PV1 was very poor. The binding assays mostly correlated with the expression of CD55 (decay accelerating factor, DAF) and α 2 β 1 -integrin (VLA-2), which are known to act as receptors for CBV3 and EV1, respectively, as well as with the expression of the PV receptor on the cell surface. To analyse virus replication in the cells, viral protein and nucleic acid syntheses were studied by immunoprecipitation and RT-PCR. CBV3 was able to replicate in Raji and Molt-4 cells, even though no expression of DAF was detected, whereas in the monocytic cell line, viral protein synthesis was detected only after transfection of virus RNA. Neither CBV3 nor EV1 replicated in PBMC and all haematopoietic cells studied seemed to be poorly permissive for PV1 replication.

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“…However, enterovirus is often also detected in the gastrointestinal or respiratory tracts during enteroviral meningitis, suggesting that the virus is very likely the cause of disease [3,5,10,12]. During viremia, enteroviruses can also be detected in the blood [1,5,[13][14][15].…”

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confidence: 99%

Diagnosis of Enteroviral Meningitis by Use of Polymerase Chain Reaction of Cerebrospinal Fluid, Stool, and Serum Specimens

et al. 2005

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The isolation of enteroviruses from blood: A comparison of four processing methods

Cited by 44 publications

References 17 publications

Antibody‐Dependent Enhancement of Coxsackievirus B4 Infectivity of Human Peripheral Blood Mononuclear Cells Results in Increased Interferon‐α Synthesis

Antibody‐Dependent Enhancement of Coxsackievirus B4 Infectivity of Human Peripheral Blood Mononuclear Cells Results in Increased Interferon‐α Synthesis

Enterovirus receptors and virus replication in human leukocytes.

Diagnosis of Enteroviral Meningitis by Use of Polymerase Chain Reaction of Cerebrospinal Fluid, Stool, and Serum Specimens

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