Enterovirus receptors and virus replication in human leukocytes.

Vuorinen, Tytti; Vainionp, R; Heino, Jyrki; Hyypi, T

doi:10.1099/0022-1317-80-4-921

Cited by 32 publications

(25 citation statements)

References 28 publications

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“…CVB3 replicated in B cells (Raji) and in T cells (Molt 4) but not in promyelocytic or monocytic cells (HL-60, U-937, and THP-1) (48). Conversely, U-937 cells were permissive for echovirus 1, but lymphocytes (Raji and Molt 4) were not (46), which is similar to what has been observed for poliovirus (15,46). While the in vivo significance of this tropism is unclear, our results are consistent with the possibility that circulating mononuclear cells could transport EV70 to other parts of the body and provide access to the CNS.…”

Section: Discussionsupporting

confidence: 88%

“…For example, echoviruses 5 and 11 replicate efficiently in PBMCs, and PBMCs also support echovirus 9 and CVA9 multiplication, although weakly (11). Results with poliovirus have been variable (14,25,46), and the CBV strains that have been examined do not appear to replicate in PBMCs (46,47,48). It will be of interest to determine if EV70 replicates in specific subsets of human PBMCs.…”

Section: Discussionmentioning

confidence: 99%

“…The inflammation that occurs in the eye during AHC presents the virus with the opportunity to infect invading white blood cells. Several studies have demonstrated that some enteroviruses can replicate in peripheral blood mononuclear cells (PBMCs) in vitro (11,14,15,46,47,48) and that differences in the susceptibility and permissiveness of lymphoid, monocytic, or myeloid cell lines correlate with the expression of viral receptors (14,15,46,47,48).…”

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confidence: 99%

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Binding to Decay-Accelerating Factor Is Not Required for Infection of Human Leukocyte Cell Lines by Enterovirus 70

Haddad

Nokhbeh

Alexander

et al. 2004

J Virol

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Enterovirus 70 (EV70) is one of several human enteroviruses that exhibit a propensity for infecting the central nervous system (CNS). The mechanisms by which neurotropic enteroviruses gain access to and invade the CNS are poorly understood. One possibility is that circulating leukocytes become infected and carry neurotropic enteroviruses to the CNS. We examined the ability of EV70 to infect cell lines derived from lymphoid, myeloid, and monocytic lineages. Most leukocyte cell lines tested bound radiolabeled EV70 and were permissive for EV70 replication, suggesting that EV70, in contrast to other enteroviruses, has an in vitro tropism that includes lymphoid, monocytic, and myeloid cell lines. For some of the cell lines, virus binding and infection correlated with surface expression of decay-accelerating factor (DAF), an attachment protein for EV70 on HeLa cells. However, EV70 also adsorbed to and infected cell lines that expressed little or no DAF.In contrast to what was observed for HeLa cells, neither DAF-specific monoclonal antibodies nor phosphatidylinositol-specific phospholipase C treatment inhibited EV70 binding to permissive leukocyte cell lines, and antibody blockade of DAF had little or no effect on EV70 replication. We also found that neither the human coxsackievirus-adenovirus receptor nor intercellular cell adhesion molecule 1, which mediate the entry of coxsackie B viruses and coxsackievirus A21, respectively, functions as a receptor for EV70. EV70 binding to all cell lines was sensitive to sialidase treatment and to inhibition of O glycosylation by benzyl N-acetyl-␣-Dgalactosaminide. Taken together, these results suggest that a sialylated molecule(s) other than DAF serves as a receptor for EV70 on permissive human leukocyte cell lines.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Binding to Decay-Accelerating Factor Is Not Required for Infection of Human Leukocyte Cell Lines by Enterovirus 70

Haddad

Nokhbeh

Alexander

et al. 2004

J Virol

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“…These findings support the concept that EV71-ADE has important implications not only for the pathogenesis of EV71 infection but also for the development of a sufficiently immunogenic EV71 vaccine. Enteroviruses can be isolated from the leukocytes of infected patients (8,11,25,34,41). CB3 can replicate in lymphocytes or monocytes (41).…”

Section: Discussionmentioning

confidence: 99%

Enterovirus 71 Infection of Monocytes with Antibody-Dependent Enhancement

Wang

Chen

Ling

et al. 2010

Clin Vaccine Immunol

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Enterovirus (EV) is an RNA virus that has circulated with different serotypes and genotypes worldwide. Enterovirus 71 (EV71) is a major neurotropic virus that causes severe brain stem encephalitis (BE) in infants and young children. The most vulnerable age for fatal infection is 6 to 11 months. This is associated with the coincident decline in maternal antibodies. The current report describes our finding that EV71 can infect human peripheral blood monocytes. We were able to show that EV71 infection is enhanced in the monocytic cell line THP-1 by the presence of subneutralizing concentrations of anti-EV71 antibodies. We also found that antibody-dependent enhancement (ADE) is mediated in part by Fc␥ receptors. These observations support the concept that ADE augments the infectivity of EV71 for human monocytes and contributes to the age-dependent pathogenesis of EV71-induced disease. The ADE phenomenon must be considered during the development of an EV71 vaccine.

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“…The different members of the DAEC pathogenic group have in common a diffusely cell-adherent phenotype mediated by the interaction of the bacterial adhesin with CD55 (13,15). It is conceivable that other human enteric pathogens, especially enteroviruses, which also use CD55 for cell entry such as coxsackieviruses or echoviruses, could behave similarly (30,31). DAF͞CD55 is a molecule already implicated in the control of innate immunity by inactivating complement C3 convertases and preventing C3b deposition on cell membranes.…”

Section: Discussionmentioning

confidence: 99%