The Isolation and Immunological Properties of Porcine Parathyroid Hormone

Abstract

Medical Research Society 7~by decay analysis, and by counting on y and fi detecting devices.The fluxes over the Garious periods during the experiment are calculated from this data. Fluxes were measured under both 'open' and 'short circuit' conditions, and were all in the range 0-150 ,uEq/cm2/min, whilst the S.C.C. was about 5-10 ,uEq/cm2/min. The effects of ADH (10-lO~) and DNP (10--5~) on the four fluxes were unable to be detected in these preliminary experiments owing to the design of the apparatus.Neutron activation was performed in conjunction with Mr M. Kerridge at the University of London Reactor, Ascot, Berks.

Metabolism of bovine parathyroid hormone. Immunological and biological characteristics of fragments generated by liver perfusion.

“…Protein was determined by the method of Lowry, Rosebrough, Farr, and Randall (29) and the adenylate cyclase activity was assessed by the method of Krishna, Weiss, and Brodie (30). The particulate fraction containing 0.09-0.12 mg protein was incubated at 370C for 15 min with 2.5 mM ATP, 3.0-3.5 X 106 cpm; theophylline, 8 mM; MgCI2, 3.0 mM; Tris-HCl, 21 mM, pH 7.7; phosphoenolpyruvate, 0.9 mM; pyruvate kinase, 0.02 mg/ml; potassium chloride, 26 mM; bovine albumin, 0.8 mg/ml; and varying amounts of bovine PTH, immunoreactive fragments, or the equivalent amount of fractionated perfusion medium. The diluent for PTH, 0.01 N acetic acid, was added to all control tubes.…”

“…Despite rapid progress in elucidating the structure of parathyroid hormone (PTH )' in several species (1)(2)(3)(4)(5), the nature of circulating PTH remains incompletely defined. The possibility that circulating PTH may consist of several distinct immunochemical forms was first suggested by the work of Berson and Yalow in 1968 (6).…”

Isolation and chemical properties of porcine parathyroid hormone

“…By use of the radioimmunoassay, it has been shown that there are immunological differences between porcine, bovine, and human parathyroid hormones (Woodhead and O'Riordan, 1970;O'Riordan and Woodhead, 1970). These differences were demonstrated by examining the ability of these three peptides to desplace antisera raised against BPTH.…”