The involvement of suppressors of cytokine signaling 2 (SOCS2) in immune defense responses of Chinese mitten crab Eriocheir sinensis

The SOCS and STAT from JAK/STAT signaling pathway of kuruma shrimp Marsupenaeus japonicus: Molecular cloning, characterization and expression analysis

“…Recently SOCS family members have been identified in few aquatic invertebrates such as Chinese mitten crab, Eriocheir sinensis [12] and abalone Haliotis discus discus [13] suggesting their involvement in immune defense mechanisms. Components of JAK/STAT pathway of crustacean species are identified, however the relation between STAT and SOCS is not well known.…”

Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

“…A total of 500 μl hemolymph in the ventral-sinus cavity was extracted from each crab using a 25-gage needle and 1.0-ml syringe that contained equal volume of anticoagulant solution (100 mmol/L glucose, 26 mmol/L citric acid, 415 mmol/L sodium chloride (NaCl), 30 mmol/L sodium citrate, 30 mmol/L ethylenediaminetetraacetic acid (EDTA); pH 4.6) (Zhang et al, 2010). In order to reduce the interindividual difference, the hemolymph obtained from five crabs was pooled together.…”

Three members in JAK/STAT signal pathway from the sea cucumber Apostichopus japonicus: Molecular cloning, characterization and function analysis

“…The SOCS box is capable of recruiting the Elongin B (EB) and Elongin C (EC) to target proteins for ubiquitination and subsequent proteosomal degradation [34,35]. Moreover, a predicted pY site (R 76 , D 77 and S 78 ) was identified in the central SH2 domain of AjSOCS2, which was the structural basis for specific phosphotyrosine recognition [19]. It has been shown that known SOCS family proteins could be divided into type I and type II subfamilies [36].…”

“…The SH2 domain in SOCS family compete with STATs-SH2 domain or hinder the STAT binding sites of receptors, inhibiting STATs activation, especially in the case of STAT5 [18]. Previous studies had shown that microorganisms can induce different SOCS proteins [19,20], but only few reports have been characterized the relationship between JAK/STAT and SOCS in invertebrate after immunostimulation [11,21]. In vertebrate, SOCS proteins have other functions.…”