The involvement of serum exosomal miR-500-3p and miR-770-3p in aging: modulation by calorie restriction

Abstract

Recent studies have shown a role for miRNAs in aging and age-related diseases, and the modulation of miRNA expression by diet attracts attention as a new therapeutic strategy. Here, we focused on identifying specific exosomal miRNAs derived from serum of aged rats and the effect of short-term calorie restriction (CR) on their expression.Exosomes from serum of young (7-month), old (22-month), and old-CR Sprague Dawley rats were isolated and characterized by transmission electron microscopy analyses, dynamic light scattering measurements, and Western blotting. A total of 12 significantly expressed miRNAs in serum exosomes of young and old rats were identified by next generation sequencing. After analysis of qRT-PCR, we found that miR-500-3p and miR-770-3p expression was significantly upregulated by aging and downregulated by CR. Furthermore, receiver operating characteristic (ROC) curve revealed that the selected miRNAs represented high accuracy in discriminating old rats from young rats. Finally, PANTHER analysis predicted selected miRNAs targets genes involved in Wnt/chemokines and cytokines -related inflammatory signaling pathway and function as transcription factor.In conclusion, our results suggest that the expression of serum exosomal miR-500-3p and miR-770-3p was significantly increased with aging, whereas these were decreased by CR, and age-/CR-modulated exosomal miR-500-3p and miR-770-3p could potentially be used as informative biomarkers candidates for aging.

Hypoxic pretreatment of adipose-derived stem cell exosomes improved cognition by delivery of circ-Epc1 and shifting microglial M1/M2 polarization in an Alzheimer’s disease mice model

“…Using a luciferase reporter system, we found in the present study that circ-Epc1 interacted with miR-770-3p. Previous studies reported that miR-770-3p expression significantly increased with aging [ 28 ]. MiR-770-3p expression can facilitate apoptosis by inducing inflammation [ 29 ].…”

Whole-Transcriptome Sequencing Analysis Reveal Mechanisms of Yiqi Huoxue Yangyin (YHY) Decoction in Ameliorating D-gal-induced Cardiac Aging

“…Combined with the analysis of KEGG, it revealed that ceRNA network mainly exert in uence on immune system, PI3K-Akt signaling pathway, and MAPK signaling pathway. For instance, miR-770 was constructed as the core in ceRNA network, while the upstream circRNAs, including 6:37353557|37364143, and lncRNAs, such as MSTRG.137663.1, MSTRG.77826.15, MSTRG.131343.148, MSTRG.80502.2, and Gm10734-201, acted as sponge to regulate Zfp831, Map3k6, Fosb, Pik3r5, Rhoh, et al Previous studies shown miR-770 is the potential candidates for investigating the aging process by control Wnt/chemokines and cytokines-related in ammation signaling pathways [40]. And miR-770 was also believed to play critical roles in the development and pathological mechanisms of dilated cardiomyopathy [41].…”

Hypoxic Pretreatment Adipose-derived Stem Cell Exosomes Improve Cognition by Delivery Circ-Epc1 and Shifting Microglial M1/M2 Polarization in Alzheimer’s Disease Mice Model

“…In this study, we found that circ-Epc1 interacted with miR-770-3p using a luciferase reporter system. The literature reports that miR-770-3p expression signi cantly increases with aging [24]. MiR-770-3p expression can facilitate apoptosis by inducing in ammation [25].…”