The involvement of mast cells in the irinotecan-induced enteric neurons loss and reactive gliosis

Nogueira, Ludmila T.; Costa, Deiziane Viana da Silva; Gomes, Antoniella S.; Martins, Carla Adriano; Silva, Angeline M. H. P.; Coelho-Aguiar, Juliana M.; Castelucci, Patrícia; Lima-Júnior, Roberto César Pereira; Leitão, Renata F. C.; Moura‐Neto, Vivaldo; Brito, Gerly Anne Castro

doi:10.1186/s12974-017-0854-1

Cited by 32 publications

(28 citation statements)

References 51 publications

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“…Several in vitro and in vivo rodent models mimicking inflammation or infection revealed the activation and plasticity of EGCs. In a chemical‐induced mucositis model using the anticancer drug irinotecan (CPT‐11) a reactive gliosis accompanied by increased GFAP and S100b expression was shown in the small intestine whereas the number of the neuronal antigen Hu‐positive neurons is decreased (Nogueira et al, ).…”

Section: Introductionmentioning

confidence: 99%

Enteric Glia: S100, GFAP, and Beyond

Grundmann

Loris

Maas-Omlor

et al. 2019

The Anatomical Record

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Since several years, the enteric nervous system (ENS) is getting more and more in the focus of gastrointestinal research. While the main interest was credited for years to the enteric neurons and their functional properties, less attention has been paid on the enteric glial cells (EGCs). Although the similarity of EGCs to central nervous system (CNS) astrocytes has been demonstrated a long time ago, EGCs were investigated in more detail only recently. Similar to the CNS, there is not "the" EGC, but also a broad range of diversity. Based on morphology and protein expression, such as glial fibrillary acidic protein (GFAP), S100, or Proteolipid-protein-1 (PLP1), several distinct glial types can be differentiated. Their heterogeneity in morphology, localization, and transcription as well as interaction with surrounding cells indicate versatile functional properties of these cells for gut function in health and disease. Although NG2 is found in a subset of CNS glial cells, it did not colocalize with the glial marker S100 or GFAP in the ENS. Instead, it in part colocalize with PDGFRα, as it does in the CNS, which do stain fibroblast-like cells in the gastrointestinal tract. Moreover, there seem to be species dependent differences. While GFAP is always found in the rodent ENS, this is completely different for the human gut.Only the compromised human ENS shows a significant amount of GFAPpositive glial cells. So, in general we can conclude that the EGC population is species specific and as complex as CNS glia. Anat Rec, 302:1333Rec, 302: -1344Rec, 302: , 2019.

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Section: Introductionmentioning

confidence: 99%

Enteric Glia: S100, GFAP, and Beyond

Grundmann

Loris

Maas-Omlor

et al. 2019

The Anatomical Record

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“…An increase in GDNF and GFAP immunolabeling was observed in EGCs in inflamed colonic mucosa of patients with UC, CD, and Clostridioides difficile (C. difficile) infectious colitis [78]. In addition, S100B upregulation has also been identified in a variety of diseases, such as UC [42,81,82], celiac disease [83], and intestinal mucositis induced by antineoplastic drugs [84,85]. Increased expression of S100B was also found in the intestine of humans with C. difficile infection (CDI), in animal model of CDI, and in mouse ileal loop injected with C. difficile toxin A (TcdA) (unpublished data).…”

Section: Egcs In Inflammatory Bowel Diseases (Ibd)mentioning

confidence: 99%

“…As we will deepen later, EGCs can be stimulated by immune cells during intestinal inflammation. A recent study showed that mediators released by mast cells cause reactive gliosis and neuronal death together with the intestinal mucositis induced by irinotecan [85]. Figure 2 shows a schematic highlighting how EGCs are affected by and participates on intestinal inflammation.…”

Section: Inflammation By Other Causesmentioning

confidence: 99%

The Enteric Glial Network Acts in the Maintenance of Intestinal Homeostasis and in Intestinal Disorders

Coelho-Aguiar¹,

Veríssimo²,

Costa³

et al. 2020

Glia in Health and Disease

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The enteric nervous system (ENS), also known as second brain, innervates our gastrointestinal tract controlling its functions, such as motility, fluid secretion, nutrient absorption, and even involvement in the control of immunity and inflammatory processes. In the gut, the gliocytes are known as enteric glial cells (EGCs). Enteric glial cells form a network that permeates the entire gut. Enteric glia express the cell surface hemichannel of connexin-43 (Cx43) necessary for the propagation of Ca2 + responses, necessary to maintain their functions. In this chapter, besides the development of ENS and its glial cells and the similarities with the astrocytes in the central nervous system, we approached the important role of the glial network in the control of gut homeostasis, in the interaction with the immune system, and its participation in pathological conditions. EGCs are even capable of replacing lost neurons. Thus the enteric glia is a multifunctional cell, which through its multiple interactions maintains the integrity of the ENS allowing it to be resistant to the different and constant aggressions suffered by the digestive system.

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“…In addition, other sectons were stained with toluidine blue solution (1 g of toluidine blue dissolved in 70% ethanol), for evaluatin of mast cells (48). Mast cell granules stain purple in color due to the presence of heparin and histamine, therefore purple stain was recognized as a positive staining for mast cells.…”

Section: Histologymentioning

confidence: 99%

“…Intensely toluidine blue-stained granulated cells, as well as cells exhibiting different degrees of degranulation that paralleled lighter staining (degranulated), were observed. The results were expressed as the percentage of mast cells degranulated by total amount (48).…”

Section: Histologymentioning

confidence: 99%

Efeito protetor do estradiol na disfunção da barreira epitelial intestinal induzida pela endotoxemia

Ribeiro¹

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A Deus, meu maior alicerce, que me concedeu força, perseverança e retidão para o término deste trabalho. Aos meus pais, Luzia e Lacerda, pelo dom da vida, pelo apoio incondicional e pelo amor único e contínuo. Obrigada meus amores, por não medirem esforços para que eu tivesse a melhor formação educacional, moral e ética, primordiais para chegar até esta fase importante da minha vida. À minha irmã Adriana, por ser minha razão nos momentos de desespero, por ser minha amiga nos dias bons e maus e por ser minha segunda mãe durante toda minha vida. Agradeço também por ter me presenteado com a minha sobrinha e afilhada linda: Sofia. Soso, a dinda te ama demais. Ao meu irmãozinho Alexsander, por ter compartilhado comigo durante parte do meu doutorado minhas inseguranças e por me dar tanto carinho, amor e doçura. Ao meu irmão Alex, pelo incentivo e por ser um exemplo para mim de excelência profissional, amo você Lekim. Ao Felipe por todo companheirismo na reta final do doutorado. Obrigada por entender e apoiar minha ausência e impaciência. Amo você. Ao meu cunhado Wainer, pelos finais de semana de muita conversa e comida boa, por ser tão gentil e prestativo comigo. À minha cunhada Mari, por todo auxílio durante o meu doutorado, obrigada por ser tão doce comigo. Ao prof. Dr. Rafael Saia pela oportunidade de trabalhar ao seu lado, pela confiança em mim depositada, pela paciência e pelos conhecimentos compartilhados que permitiram a conclusão deste trabalho. Obrigada por todas as conquistas proporcionadas nesses anos de convivência e trabalho. Agradeço imensamente ao prof. Dr. Celso Rodrigues Franci por todo apoio científico que me deu ao longo de toda a realização do trabalho e o empenho fundamental demonstrado na fase de finalização da tese. Obrigada por todo respeito, confiança, críticas e incentivo. À Prof a. Dr a. Janete Franci, por ter me iniciado à vida científica. Sou grata pelas oportunidades e conhecimentos obtidos durante todos os anos que estive ao seu lado. Ao Humberto Giusti pela convivência agradável, por ser tão solícito e por todos conselhos que levarei para minha vida pessoal e profissional. Obrigada pelo suporte técnico impecável. À Prof a. Dr a. Leda Menescal de Oliveira pelo respeito e carinho depositado à mim, disponibilização da infraestrutura e parceria em diferentes projetos. Ao meu amigo de pós-graduação e da vida Procópio Filho, por ser a melhor pessoa que pude conviver durante toda minha vida. Obrigada por me aguentar todos os dias, sendo nos momentos de alegria ou de tristeza. Amo você, meu amigo. À minha amiga amada Aninha Omoto, por compartilhar comigo todos os momentos na pós-graduação e fora dela. Tenho muito carinho por você e jamais esquecerei tudo o que vivemos até aqui. "Aqueles que passam por nós, não vão sós, não nos deixam sós. Deixam um pouco de si, levam um pouco de nós. " Antoine de Saint-Exupéry Agradeço aos professores participantes da banca examinadora pela disponibilidade em participarem e contribuírem neste momento tão importante e

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The involvement of mast cells in the irinotecan-induced enteric neurons loss and reactive gliosis

Cited by 32 publications

References 51 publications

Enteric Glia: S100, GFAP, and Beyond

Enteric Glia: S100, GFAP, and Beyond

The Enteric Glial Network Acts in the Maintenance of Intestinal Homeostasis and in Intestinal Disorders

Efeito protetor do estradiol na disfunção da barreira epitelial intestinal induzida pela endotoxemia

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