The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber

Zhao, Zongxi; Li, Changxia; Liu, Huwei; Yang, Jingjing; Huang, Panpan; Liao, Weibiao

doi:10.3390/plants10091937

Cited by 15 publications

(8 citation statements)

References 43 publications

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“…This study found that 50% HRW could reduce the starch content and increase the content of water-soluble carbohydrate, sucrose, glucose, and fructose in Lanzhou lily scales (Figure 5). HRW treatment promoted the occurrence of cucumber adventitious roots by increasing glucose, sucrose, and water-soluble carbohydrate content [41]. Huang et al [38] found that HRW could accelerate starch degradation and increase sugar accumulation to promoted seed germination in cucumber.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose content was determined according to Zhao et al [41]. Lily scales (0.5 g) were thoroughly ground with distilled water.…”

Section: Measurement Of Starch Water-soluble Carbohydrate Sucrose Glu...mentioning

confidence: 99%

“…In addition, water-soluble carbohydrate is also an osmotic regulator and accumulates more monosaccharides under drought stress, thereby increasing intracellular water content [40]. It was reported that HRW increased the content of glucose, sucrose, and water-soluble carbohydrate by increasing the activity of sucrose synthesis-related enzymes and glucoserelated enzymes during the occurrence of adventitious roots in cucumber [41].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

et al. 2023

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Hydrogen gas (H2) is considered as a signaling molecule and plays multiple roles in plant growth. However, the effect of H2 on postharvest physiology in lily scales during storage has not been reported. In this study, the regulatory roles of hydrogen-rich water (HRW, a H2 donor, a concentration of 0.45 mM for 100% HRW) in water status, ion balance, and nutrients in Lanzhou lily (Lilium davidii var. unicolor) scales were investigated. The scales were soaked in HRW for 12 d, and sampling was performed every 3 d for a total of 5 times. The results show that HRW (0, 10, 50, and 100%) increased the fresh weight, dry weight, relative water content, and water loss rate in lily scales, with maximum biological response at 50% HRW. Treatment with 50% HRW significantly increased the K+ content and K+/Na+ ratio in lily scales and decreased Na+ content. The Na+ K+-ATPase, and PM H+-ATPase activities were also increased by 50% HRW treatment. Meanwhile, 50% HRW up-regulated the expression of AKT1 and HA3 genes and down-regulated the expression of NHX2 and SOS1 genes. In addition, 50% HRW treatment significantly increased the expression level of PIP1;5, PIP2A, TIP1;3, and TIP2;2 genes. Treatment with 50% HRW significantly increased the content of water-soluble carbohydrate, sucrose, glucose, and fructose in lily scales, and decreased the content of starch. In addition, 50% HRW treatment significantly increased the activity of α-amylase, β-amylase, total amylase, sucrose synthase, and sucrose phosphate synthase. Collectively, H2 might enhance the water retention capacity and nutrient content in lily scales by maintaining ion balance, regulating aquaporin, and increasing sugar-metabolizing enzyme activity, thereby prolonging the storage period of postharvest scales of Lanzhou lily.

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Section: Discussionmentioning

confidence: 99%

“…Glucose content was determined according to Zhao et al [41]. Lily scales (0.5 g) were thoroughly ground with distilled water.…”

Section: Measurement Of Starch Water-soluble Carbohydrate Sucrose Glu...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

et al. 2023

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“…After this step, the seeds in the tray were moved to the incubator with illumination with a 14 h photoperiod (the photosynthetically active radiation was 200 μmol m −2 s −1 ) at 25 ± 1 °C for 7 d, and the filter paper was kept wet in the tray during this period. The primary roots of explants were carefully cut off after 7 d. In accordance with Zhao et al [ 57 ], the induction of adventitious roots was completed 48 h after treatment. Hence, the cucumber explants were placed in Petri dishes with different treatments and allowed to grow for 48 h. The temperature and photoperiod conditions were the same as the above conditions.…”

Section: Methodsmentioning

confidence: 99%

Identification of Key Genes during Ethylene-Induced Adventitious Root Development in Cucumber (Cucumis sativus L.)

Deng

Wang

Zhang

et al. 2022

IJMS

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Ethylene (ETH), as a key plant hormone, plays critical roles in various processes of plant growth and development. ETH has been reported to induce adventitious rooting. Moreover, our previous studies have shown that exogenous ETH may induce plant adventitious root development in cucumber (Cucumis sativus L.). However, the key genes involved in this process are still unclear. To explore the key genes in ETH-induced adventitious root development, we employed a transcriptome technique and revealed 1415 differentially expressed genes (DEGs), with 687 DEGs up-regulated and 728 DEGs down-regulated. Using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, we further identified critical pathways that were involved in ETH-induced adventitious root development, including carbon metabolism (starch and sucrose metabolism, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), oxidative phosphorylation, fatty acid biosynthesis, and fatty acid degradation), secondary metabolism (phenylalanine metabolism and flavonoid biosynthesis) and plant hormone signal transduction. In carbon metabolism, ETH reduced the content of sucrose, glucose, starch, the activity of sucrose synthase (SS), sucrose–phosphate synthase (SPS) and hexokinase (HK), and the expressions of CsHK2, pyruvate kinase 2 (CsPK2), and CsCYP86A1, whereas it enhanced the expressions of β-amylase 1 (CsBAM1) and β-amylase 3 (CsBAM3). In secondary metabolism, the transcript levels of phenylalanine ammonia-lyase (CsPAL) and flavonoid 3′-monooxygenase (CsF3′M) were negatively regulated, and that of primary-amine oxidase (CsPAO) was positively regulated by ETH. Additionally, the indole-3-acetic acid (IAA) content and the expressions of auxin and ETH signaling transduction-related genes (auxin transporter-like protein 5 (CsLAX5), CsGH3.17, CsSUAR50, and CsERS) were suppressed, whereas the abscisic acid (ABA) content and the expressions of ABA and BR signaling transduction-related genes (CsPYL1, CsPYL5, CsPYL8, BRI1-associated kinase 1 (CsBAK1), and CsXTH3) were promoted by ETH. Furthermore, the mRNA levels of these genes were confirmed by real-time PCR (RT-qPCR). These results indicate that genes related to carbon metabolism, secondary metabolite biosynthesis, and plant hormone signaling transduction are involved in ETH-induced adventitious root development. This work identified the key pathways and genes in ETH-induced adventitious rooting in cucumber, which may provide new insights into ETH-induced adventitious root development and will be useful for investigating the molecular roles of key genes in this process in further studies.

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“…The mechanisms in the cells, which enable H 2 to have its effects, were also the focus of papers in this SI. Zhao et al [ 25 ] show an interaction between H 2 and glucose in adventitious roots of cucumber. The effects of HRW were blocked by glucosamine, suggesting that glucose content may be mediating root development.…”

Section: Mechanisms Of H 2 Actionmentioning

confidence: 99%

Editorial for Special Issue: “Production and Role of Molecular Hydrogen in Plants”

Hancock

2022

Plants

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The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber

Cited by 15 publications

References 43 publications

Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

Identification of Key Genes during Ethylene-Induced Adventitious Root Development in Cucumber (Cucumis sativus L.)

Editorial for Special Issue: “Production and Role of Molecular Hydrogen in Plants”

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