The involvement of galectin-1 in skeletal muscle determination, differentiation and regeneration

Watt, Diana J.; Jones, Gareth E.; Goldring, Kirstin

doi:10.1023/b:glyc.0000014093.23509.92

Cited by 45 publications

(61 citation statements)

References 34 publications

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“…Interestingly, enhanced expression of ␣7␤1 integrin has been shown to prevent the onset of the dystrophic process in the mouse model for Duchenne muscular dystrophy (Burkin et al, 2001). These data suggest a role for galectin-1 in muscle differentiation, and this role is substantiated by several lines of evidence that show an increased fusion of myoblasts to the multinucleate muscle fiber stage in vitro in the presence of the lectin (Gartner and Podleski, 1975;Nowak et al, 1976;Watt et al, 2004). In both chick (Nowak et al, 1976) and mouse (Poirier et al, 1992) skeletal muscle development, the level of galectin-1 is highest during the maximum stage of myoblast fusion, implicating a role for the molecule in the myoblast recognition-fusion process (Nowak et al, 1976).…”

Section: Introductionmentioning

confidence: 85%

“…However, a detailed examination of skeletal muscle of the null mouse had never been undertaken. Our preliminary data suggested that regeneration of skeletal muscle in response to trauma is somewhat attenuated in the galectin-1 null mouse (Watt et al, 2004). In the light of these preliminary data, we undertook a more detailed study of galectin-1 null muscle by studying the response of galectin-1 null myoblasts in culture, the development of galectin-1 null muscle, and lastly the regenerative response of this muscle to traumatic injury.…”

Section: Introductionmentioning

confidence: 99%

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Lack of galectin‐1 results in defects in myoblast fusion and muscle regeneration

Georgiadis

Stewart

Pollard

et al. 2007

Developmental Dynamics

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Galectin-1 has been implicated in the development of skeletal muscle, being maximally expressed at the time of myofiber formation. Furthermore, in the presence of exogenous galectin-1, mononuclear myoblasts show increased fusion in vitro. In the current study, we have used the galectin-1 null mouse to elucidate the role of galectin-1 in skeletal muscle development and regeneration. Myoblasts derived from the galectin-1 mutant showed a reduced ability to fuse in vitro. In galectin-1 null mutants, there was evidence of a delay in muscle fiber development at the neonatal stage and muscle fiber diameter was reduced when compared with wild-type at the adult stage. Muscle regeneration was also compromised in the galectin-1 mutant with the process being delayed and a reduced fiber size being maintained. These results, therefore, show a definitive role for galectin-1 in fusion of myoblasts both in vitro, in vivo, and in regeneration after recovery from induced injury. Developmental Dynamics 236:1014 -1024, 2007.

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Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Lack of galectin‐1 results in defects in myoblast fusion and muscle regeneration

Georgiadis

Stewart

Pollard

et al. 2007

Developmental Dynamics

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“…43 LGALS1 enhances the fusion of myoblasts in myotubes and this explains why galectin-1 is expressed at high levels when maximal cell fusion occurs during muscle development. 44 Bridgingintegrator protein-1 isoform BIN1112A (BIN1) is a nucleocytoplasmic adaptor protein that interacts at the Myc box region at the amino terminus of the c-myc protein. Cells overexpressing human BIN1 grow more slowly than control myoblasts, and they differentiate more rapidly when depleted of growth factors.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling identifies potential relevant genes in alveolar rhabdomyosarcoma pathogenesis and discriminates PAX3‐FKHR positive and negative tumors

Pittà

Tombolan

Albiero

et al. 2006

Intl Journal of Cancer

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We analyzed the expression signatures of 14 tumor biopsies from children affected by alveolar rhabdomyosarcoma (ARMS) to identify genes correlating to biological features of this tumor. Seven of these patients were positive for the PAX3-FKHR fusion gene and 7 were negative. We used a cDNA platform containing a large majority of probes derived from muscle tissues. The comparison of transcription profiles of tumor samples with fetal skeletal muscle identified 171 differentially expressed genes common to all ARMS patients. The functional classification analysis of altered genes led to the identification of a group of transcripts (LGALS1, BIN1) that may be relevant for the tumorigenic processes. The muscle-specific microarray platform was able to distinguish PAX3-FKHR positive and negative ARMS through the expression pattern of a limited number of genes (RAC1, CFL1, CCND1, IGFBP2) that might be biologically relevant for the different clinical behavior and aggressiveness of the 2 ARMS subtypes. Expression levels for selected candidate genes were validated by quantitative real-time reverse-transcription PCR. ' 2005 Wiley-Liss, Inc.Key words: alveolar rhabdomyosarcoma; microarray; gene expression profiling; PAX3-FKHR Rhabdomyosarcomas (RMS) are rare but very aggressive tumors of childhood. It is generally hypothesized that these tumors arise as a consequence of regulatory disruption of the growth and differentiation pathways of myogenic precursor cells, but the actual identity of this precursor is still a matter of debate and research. 1,2 Based on morphology, 2 major RMS subtypes can be identified: embryonal RMS (ERMS) and alveolar RMS (ARMS). The embryonal RMS includes also the botryoid, anaplastic and spindle-cell variants. An additional subtype is the pleomorphic RMS, which is exceptionally found in childhood. 3 ARMS represents approximately 25-30% of RMS and has a worse prognosis than ERMS. Cytogenetic and molecular analyses have demonstrated that ARMS frequently harbor the reciprocal chromosomal translocation t(2;13)(q35;q14), in which the PAX3 and FKHR genes are juxtaposed; also the less frequent variant translocation t(1;13)(p36;q14) has been associated to ARMS. The PAX3-FKHR chimeric protein binds regulatory sequences of genes involved in growth, differentiation, apoptosis and in vivo migration of rhabdomyoblasts, including c-met, IGF-1, PDGF-R, BCL-X, SDF-1 and CXCR4. [4][5][6][7][8] Moreover, retrospective analysis of PAX3-FKHR positive and negative ARMS 9-11 suggested that the translocation positive ARMS patients fared worse than the negative counterpart. Thus, the presence of the t(2;13) reciprocal translocation should be considered one of the main features affecting the biology of ARMS cells and might represent an adverse prognostic factor.Recently, the genomic approach based on global gene expression profiling by DNA microarrays has seen an explosive number of applications for cancer classification, prognosis and functional analysis of gene networks implicated in cancer biology. [12][13][14] Among a num...

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“…LGALS1 appears to play a role in a broad array of cell functions, including cell adhesion, migration, apoptosis, differentiation, nerve and muscle regeneration, and immunoregulation (Almkvist and Karlsson, 2004;Watt et al, 2004;Jiang et al, 2005;Liu, 2005). LGALS1 has also been associated with an anti-inflammatory function (Chung et al, 2000;La et al, 2003).…”

Section: Activity Of Immune Response/inflammationrelated Genes Duringmentioning

confidence: 99%

Global analysis of gene expression inXenopushindlimbs during stage‐dependent complete and incomplete regeneration

Grow

Neff

Mescher

et al. 2006

Developmental Dynamics

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Xenopus laevis tadpoles are capable of limb regeneration after amputation, in a process that initially involves the formation of a blastema. However, Xenopus has full regenerative capacity only through premetamorphic stages. We have used the Affymetrix Xenopus laevis Genome Genechip microarray to perform a large-scale screen of gene expression in the regeneration-complete, stage 53 (st53), and regeneration-incomplete, stage 57 (st57), hindlimbs at 1 and 5 days postamputation. Through an exhaustive reannotation of the Genechip and a variety of comparative bioinformatic analyses, we have identified genes that are differentially expressed between the regeneration-complete and -incomplete stages, detected the transcriptional changes associated with the regenerating blastema, and compared these results with those of other regeneration researchers. We focus particular attention on striking transcriptional activity observed in genes associated with patterning, stress response, and inflammation. Overall, this work provides the most comprehensive views yet of a regenerating limb and different transcriptional compositions of regeneration-competent and deficient tissues.

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The involvement of galectin-1 in skeletal muscle determination, differentiation and regeneration

Cited by 45 publications

References 34 publications

Lack of galectin‐1 results in defects in myoblast fusion and muscle regeneration

Lack of galectin‐1 results in defects in myoblast fusion and muscle regeneration

Gene expression profiling identifies potential relevant genes in alveolar rhabdomyosarcoma pathogenesis and discriminates PAX3‐FKHR positive and negative tumors

Global analysis of gene expression inXenopushindlimbs during stage‐dependent complete and incomplete regeneration

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