Lack of galectin‐1 results in defects in myoblast fusion and muscle regeneration

Georgiadis, Vasilios; Stewart, Helen; Pollard, Hilary; Tavsanoglu, Yasemin; Prasad, Rathi; Horwood, Julia; Deltour, Louise; Goldring, Kirstin; Poirier, Françoise; Lawrence-Watt, Diana J.

doi:10.1002/dvdy.21123

Cited by 67 publications

(62 citation statements)

References 62 publications

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“…Recently, it has been reported that a lack of galectin-1 results in defects in myoblast fusion and muscle regeneration after cardiotoxin injury. 27 Taken together, we may suggest that galectin-1 plays an important role during tissue regeneration in response to insults in general.…”

Section: Discussionmentioning

confidence: 74%

Galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the dentate gyrus of adult mouse hippocampus

et al. 2008

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We examined the expression of galectin-1, an endogenous lectin with one carbohydrate-binding domain, in the adult mouse hippocampus after systemic kainate administration. We found that the expression of galectin-1 was remarkably increased in activated astrocytes of the CA3 subregion and dentate gyrus of the hippocampus, and in nestin-positive neural progenitors in the dentate gyrus. Quantitative reverse transcription PCR (RT-PCR) analysis revealed that the galectin-1 mRNA level in hippocampus began to increase 1 day after kainate administration and that a 13-fold increase was attained within 3 days. Western blotting analysis confirmed that the level of galectin-1 protein increased to more than three-fold a week after the exposure. We showed that isolated astrocytes express and secrete galectin-1. To clarify the significance of the increased expression of galectin-1 in hippocampus, we compared the levels of hippocampal cell proliferation in galectin-1 knockout and wild-type mice after saline or kainate administration. The number of 5-bromo-2 0 -deoxyuridine (BrdU)-positive cells detected in the subgranular zone (SGZ) of galectin-1 knockout mice decreased to 62% with saline, and to 52% with kainate, as compared with the number seen in the wild-type mice. Most of the BrdU-positive cells in SGZ expressed doublecortin and neuron-specific nuclear protein, indicating that they are immature neurons. We therefore concluded that galectin-1 promotes basal and kainateinduced proliferation of neural progenitors in the hippocampus.

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Section: Discussionmentioning

confidence: 74%

Galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the dentate gyrus of adult mouse hippocampus

et al. 2008

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“…Gal1 has been suggested to play a role in skeletal muscle development [3][4][5][6]. During muscle differentiation, Gal1 is secreted and interacts with laminin in the basement membrane [4].…”

Section: Discussionmentioning

confidence: 99%

“…This interaction has been suggested to inhibit myoblast attachment to laminin and hence fusion into myofibers [4]. However, other studies have shown a reduction in the fusion of Gal1 null myoblasts and an impaired regeneration of adult muscle fibers in Gal1 null mutant mice [5,6]. Consequently, Gal1 has been suggested to enhance skeletal muscle growth during development and adult muscle regeneration [6,22].…”

Section: Discussionmentioning

confidence: 99%

“…Gal1 exists both in the cytosol and extracellularly [1,2], and has been implicated in skeletal muscle development [3][4][5][6]. In the neuromuscular system, Gal1 may also play a role in neuronal degeneration and/or regeneration: Gal1 null mutant mice show a reduced rate of recovery of whisking movements after facial nerve crush [7] and delayed elimination of nerve endings following sciatic nerve section [8].…”

Section: Introductionmentioning

confidence: 99%

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Galectin-1 expression in innervated and denervated skeletal muscle

Svensson¹,

Tågerud²

2009

Cellular and Molecular Biology Letters

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Galectin-1 is a soluble carbohydrate-binding protein with a particularly high expression in skeletal muscle. Galectin-1 has been implicated in skeletal muscle development and in adult muscle regeneration, but also in the degeneration of neuronal processes and/or in peripheral nerve regeneration. Exogenously supplied oxidized galectin-1, which lacks carbohydrate-binding properties, has been shown to promote neurite outgrowth after sciatic nerve sectioning. In this study, we compared the expression of galectin-1 mRNA and immunoreactivity in innervated and denervated mouse and rat hind-limb and hemidiaphragm muscles. The results show that galectin-1 mRNA expression and immunoreactivity are up-regulated following denervation. The galectin-1 mRNA is expressed in the extrasynaptic and perisynaptic regions of the muscle, and its immunoreactivity can be detected in both regions by Western blot analysis. The results are compatible with a role for galectin-1 in facilitating reinnervation of denervated skeletal muscle.

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“…Ainsi, chez les souris dépourvues de galectine 7, on observe un retard de cicatrisation après une blessure et une prolifération anormale des kératinocytes après une exposition aux rayons UVB, indiquant que cette galectine intervient dans la répa-ration de l'épiderme [17]. De la même façon, la galectine 1 est impliquée dans la régéné-ration musculaire déclenchée par une blessure induite par l'injection de cardiotoxine [18]. Plus généralement, certaines galectines auraient un rôle dans le maintien des cellules souches musculaires et neuronales.…”

Section: Fonctions Des Galectinesunclassified