The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe

Raspor, Peter; Fujs, Štefan; Bánszky, Luca; Maráz, Anna; Batič, Martin

doi:10.1007/s00253-003-1336-1

Cited by 18 publications

(15 citation statements)

References 32 publications

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“…The increased amino acid content at various Se concentrations might be due to the synthesis of non-protein selenoamino acids. Higher values of total amino acid content in leaves with selenite treatments as compared to selenate treatments might be attributed to the fact that selenate reduction by ATP sulfurylase is a ratelimiting step (Terry et al 2000;Raspor et al 2003). Peterson and Butler (1962) suggested that Se accumulator species have evolved a mechanism whereby Se may be excluded from protein incorporation.…”

Section: Discussionmentioning

confidence: 93%

Comparative effects of selenate and selenite on growth and biochemical composition of rapeseed (Brassica napus L.)

et al. 2009

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High levels of selenium can cause adverse effects in plants as well as animals. In a greenhouse experiment, rapeseed (Brassica napus) was grown in an alkaline sandy loam soil treated with different levels of selenate-Se and selenite-Se ranging from 0 to 4 mg kg −1 . Total dry matter yield of selenium-treated rapeseed plants decreased significantly as compared to control plants. Plants were stressed at a very early stage of vegetative growth and produced fewer rosettes and flowers. Plant height and leaf production were negatively affected by selenate-Se. Dry matter of leaves was significantly higher in selenite-than in selenate-treated plants. Selenate-treated plants accumulated 75-160 times more Se in shoots and 2-18 times more in roots as compared to selenite-treated plants at the rosette formation stage, with this difference narrowing at peak flowering stage. Rapeseed leaves were subjected to biochemical analysis at rosette and peak flowering stages. Accumulation of selenium in leaves resulted in a significant increase in lipid peroxidation, chlorophyll, vitamin C and free amino acids, and a decrease in phenols, total soluble sugars and starch concentration.

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Section: Discussionmentioning

confidence: 93%

Comparative effects of selenate and selenite on growth and biochemical composition of rapeseed (Brassica napus L.)

et al. 2009

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“…In lower forms of life, selenite, selenate and selenomethionine are all reported to be toxic [1]–[3], [33], [45]. In animals the toxicity of different inorganic Se forms is ranked: selenide (H 2 Se gas)>>selenite>selenate [4], [5], and organic forms such as selenomethionine and selenocysteine are less toxic than inorganic forms.…”

Section: Discussionmentioning

confidence: 99%

Deletion of Thioredoxin Reductase and Effects of Selenite and Selenate Toxicity in Caenorhabditis elegans

2013

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Thioredoxin reductase-1 (TRXR-1) is the sole selenoprotein in C. elegans, and selenite is a substrate for thioredoxin reductase, so TRXR-1 may play a role in metabolism of selenium (Se) to toxic forms. To study the role of TRXR in Se toxicity, we cultured C. elegans with deletions of trxr-1, trxr-2, and both in axenic media with increasing concentrations of inorganic Se. Wild-type C. elegans cultured for 12 days in Se-deficient axenic media grow and reproduce equivalent to Se-supplemented media. Supplementation with 0–2 mM Se as selenite results in inverse, sigmoidal response curves with an LC50 of 0.20 mM Se, due to impaired growth rather than reproduction. Deletion of trxr-1, trxr-2 or both does not modulate growth or Se toxicity in C. elegans grown axenically, and 75Se labeling showed that TRXR-1 arises from the trxr-1 gene and not from bacterial genes. Se response curves for selenide (LC50 0.23 mM Se) were identical to selenite, but selenate was 1/4th as toxic (LC50 0.95 mM Se) as selenite and not modulated by TRXR deletion. These nutritional and genetic studies in axenic media show that Se and TRXR are not essential for C. elegans, and that TRXR alone is not essential for metabolism of inorganic Se to toxic species.

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“…APSe is unstable and rapidly dissociates in vitro into AMP and selenate in a reaction termed selenolysis, which has been used as a measure of ATP sulfurylase activity (Renosto et al 1993). Null mutations in the gene encoding ATP sulfurylase in Schizosaccharomyces pombe resulted in increased selenate tolerance and a 10-fold increase in Se accumulation Raspor et al 2003). These selenateresistant mutants display low ATP sulfurylase activity, suggesting that the activation and subsequent reduction of sulfate and selenate is important for their accumulation.…”

Section: Atp Sulfurylasementioning

confidence: 99%

Selenium uptake, translocation, assimilation and metabolic fate in plants

2005

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The chemical and physical resemblance between selenium (Se) and sulfur (S) establishes that both these elements share common metabolic pathways in plants. The presence of isologous Se and S compounds indicates that these elements compete in biochemical processes that affect uptake, translocation and assimilation throughout plant development. Yet, minor but crucial differences in reactivity and other metabolic interactions infer that some biochemical processes involving Se may be excluded from those relating to S. This review examines the current understanding of physiological and biochemical relationships between S and Se metabolism by highlighting their similarities and differences in relation to uptake, transport and assimilation pathways as observed in Se hyperaccumulator and non-accumulator plant species. The exploitation of genetic resources used in bioengineering strategies of plants is illuminating the function of sulfate transporters and key enzymes of the S assimilatory pathway in relation to Se accumulation and final metabolic fate. These strategies are providing the basic framework by which to resolve questions relating to the essentiality of Se in plants and the mechanisms utilized by Se hyperaccumulators to circumvent toxicity. In addition, such approaches may assist in the future application of genetically engineered Se accumulating plants for environmental renewal and human health objectives.

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The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe

Cited by 18 publications

References 32 publications

Comparative effects of selenate and selenite on growth and biochemical composition of rapeseed (Brassica napus L.)

Comparative effects of selenate and selenite on growth and biochemical composition of rapeseed (Brassica napus L.)

Deletion of Thioredoxin Reductase and Effects of Selenite and Selenate Toxicity in Caenorhabditis elegans

Selenium uptake, translocation, assimilation and metabolic fate in plants

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