The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

Saka, S.; Aouacheri, O.

doi:10.4172/jbb.1000327

Cited by 13 publications

(10 citation statements)

References 17 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported the present of methotrexate crystals in the renal tubules especially when it is administrated at high doses cause severe renal toxicity and acute renal failure considering the most important side effect (6). Methotrexate increases the reactive oxygen species in renal tissues; therefor it has been reported to reduce the methionine synthesis and antioxidant enzymes such as catalase and glutathione peroxidase (7).…”

Section: Introductionmentioning

confidence: 99%

Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats

Alabdaly¹,

Saeed²,

Al-hashemi³

2021

IJVS

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats

Alabdaly¹,

Saeed²,

Al-hashemi³

2021

IJVS

View full text Add to dashboard Cite

“…Previous investigations reported that MTX can promote ROS production by inducing mitochondrial dysfunction and activating NADPH oxidases, which generate excessive amounts of ROS, especially superoxide anion, as their main products (22) . The resultant oxidative burst, coupled with impairment of the antioxidant defences of the body; can result in oxidative damage of important cellular components including lipids, proteins, and nucleic acids; contributing to lipid peroxidation and cell death (22,30) .…”

Section: Discussionmentioning

confidence: 99%

The Ameliorative Effect of Fimasartan against Methotrexate-Induced Nephrotoxicity in Rats

Rasheed

Hassan²

2022

IJPS

View full text Add to dashboard Cite

Drug-induced acute kidney injury is a serious disorder. Oxidative stress has a key role in its initiation and progression. In this study, the possible ameliorative effect of fimasartan against methotrexate-induced nephrotoxicity was investigated in comparison with α-tocopherol in rats. Wistar rats were allocated into six groups and treated as follows: group Ӏ received water on a daily basis for 8 successive days; group ӀӀ received methotrexate (20 mg/kg) on day 1, followed by water for 7 successive days; group ӀӀӀ received fimasartan (3 mg/kg/day) for 7 successive days; group IV received α-tocopherol (1 g/kg/day) for 7 successive days; group V received methotrexate (20 mg/kg) on day 1, followed by fimasartan (3 mg/kg/day) for 7 successive days; and group VI received methotrexate (20 mg/kg) on day 1, followed by α-tocopherol (1 g/kg/day) for 7 successive days. Finally, after euthanization of each animal by diethyl ether, the samples were collected for analysis. Administration of fimasartan and α-tocopherol resulted in a significant decline in serum creatinine and urea, a significant reduction of renal malondialdehyde, and a significant elevation of renal superoxide dismutase-1 compared to the methotrexate-treated rats. In conclusion, fimasartan has ameliorative effects, comparable to those of α-tocopherol, on methotrexate-induced nephrotoxicity in rats.

show abstract

“…MTX has been shown to damage the structure of the testes and seminiferous tubules as well as sperm DNA, reducing the sperm count (Khayat Nouri et al, 2010; Padmanabhan et al, 2009; Serati Nouri et al, 2009). There is evidence that MTX induces oxidative stress and increase reactive oxygen species (ROS) in testicular tissue (Saka & Aouacheri, 2017), which has been associated with spermatocyte apoptosis and atrophy of seminiferous tubules (Yuluğ et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Selenium mitigates methotrexate‐induced testicular injury: Insights from male NMRI mice model

Gholami,

Nemati,

Zarasvand

et al. 2024

Birth Defects Research

View full text Add to dashboard Cite

Background and AimChemotherapy, particularly with methotrexate (MTX), often elicits testicular toxicity, leading to impaired spermatogenesis and hormone imbalances. This study aimed to investigate the potential protective effects of selenium (Se) against MTX‐induced testicular injury.Materials and MethodsMale mice were divided into control, MTX, Se, and MTX + Se groups. Histopathological examination involved the preparation of testicular tissue sections using the Johnsen's tubular biopsy score (JTBS) for spermatogenesis evaluation. Biochemical tests included the assessment of testosterone, malondialdehyde (MDA), luteinizing hormone (LH), and follicle‐stimulating hormone (FSH) levels. Real‐time quantitative polymerase chain reaction (RT‐qPCR) was employed to analyze the expression of caspase 3 (casp3), tumor protein 53 (p53), B‐cell lymphoma 2 (Bcl2), and Bcl2‐associated X protein (Bax) genes. Statistical analysis was performed using ANOVA and Tukey's tests (p < .05).ResultsHistopathological analysis revealed significant testicular damage in the MTX group, with decreased spermatogenesis and Leydig cell count, while Se administration mitigated these effects, preserving the structural integrity of the reproductive epithelium. Biochemical analysis demonstrated that MTX led to elevated malondialdehyde (MDA) levels and reduced testosterone, LH, and FSH levels, suggesting oxidative stress and Leydig cell dysfunction. Gene expression analysis indicated that MTX upregulated proapoptotic genes (casp3, p53, and bax) while downregulating the antiapoptotic Bcl2 gene. In contrast, Se treatment reversed these trends, highlighting its potential antiapoptotic properties.ConclusionOur findings underscore the potential of Se as a therapeutic agent to mitigate the reproductive toxicity associated with MTX‐induced testicular injury. Se exerts protective effects by regulating oxidative stress, preserving hormone balance, and modulating apoptotic pathways. These results suggest that Se supplementation could be a promising strategy to alleviate chemotherapy‐induced testicular damage and preserve male fertility.

show abstract

The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

Cited by 13 publications

References 17 publications

Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats

Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats

The Ameliorative Effect of Fimasartan against Methotrexate-Induced Nephrotoxicity in Rats

Selenium mitigates methotrexate‐induced testicular injury: Insights from male NMRI mice model

Contact Info

Product

Resources

About