The investigation of recognition interaction between phenylboronate monolayer and glycated hemoglobin using surface plasmon resonance

Monitoring conformational changes of immobilized RNase A and Lysozyme in reductive unfolding by surface plasmon resonance

“…This result gives us an insight into that a collapsed intermediate might exist in protein refolding, which the looser random coil in denatured state changes to the structural packing in native state. Thus, this study will provide unique conformational information of proteins on the solid interfaces, even extend to elucidate and characterize structural changes of biomolecules, including proteins, DNA and polysaccharides, in sensor and molecular recognition fields [29,4].…”

“…However, we can never be too careful to interpret the high throughput data generated in the early stages of application of BIA techniques, especially in signal variation from a conformational isomerization of probe molecules [1]. Therefore, understanding of protein conformations at an interface is essential for studies of membrane proteins, signal transduction, medical and industrial applications [2][3][4].…”

Supersensitive detection of T-2 toxin by the in situ synthesized π-conjugated molecularly imprinted nanopatterns. An in situ investigation by surface plasmon resonance combined with electrochemistry

“…In the SPR system, the signal R is proportional to the amount of [AB] and the R max is proportional to the initial [B] (Liu et al, 2008). Thus, the affinity measurement for rebinding of T-2 with T-2 MIP was deduced by calculating K D and B max using kinetic evaluation software and presented as Fig.…”

Determination of glycated hemoglobin on the basis of spectral shifting from protein-dye interaction

“…The 1,2-/1,3-diol groups of boronic acids bind covalently to the cis-diol group of glycoproteins. This interaction has been utilized in many fields of research including analyses and separations of diol-containing biomolecules [8][9][10] . In analytical fields, probes based on boronic acid functionality have found many applications [11][12][13][14] .…”