Determination of glycated hemoglobin on the basis of spectral shifting from protein-dye interaction

Kim, Eun Joong; Song, Seung Yeon; Lee, Hyun Ju

doi:10.1007/s13206-010-4103-2

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Boronic acids show affinity for this glycated moiety, and boronate affinity chromatographies are practically used for the determination of HbA 1c [ 92 – 94 ]. Kim et al have developed a method for the detection of glycated protein on the basis of the spectral shifting of dye 5 [ 95 ]. The measurements revealed that the method has a dynamic detection range of 3%–15% (HbA 1c /total hemoglobin), which covers the required clinical reference range.…”

Section: Other Target Molecules For Boronic Acid Azobenzenesmentioning

confidence: 99%

Colorimetric Sugar Sensing Using Boronic Acid-Substituted Azobenzenes

2014

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In association with increasing diabetes prevalence, it is desirable to develop new glucose sensing systems with low cost, ease of use, high stability and good portability. Boronic acid is one of the potential candidates for a future alternative to enzyme-based glucose sensors. Boronic acid derivatives have been widely used for the sugar recognition motif, because boronic acids bind adjacent diols to form cyclic boronate esters. In order to develop colorimetric sugar sensors, boronic acid-conjugated azobenzenes have been synthesized. There are several types of boronic acid azobenzenes, and their characteristics tend to rely on the substitute position of the boronic acid moiety. For example, o-substitution of boronic acid to the azo group gives the advantage of a significant color change upon sugar addition. Nitrogen-15 Nuclear Magnetic Resonance (NMR) studies clearly show a signaling mechanism based on the formation and cleavage of the B–N dative bond between boronic acid and azo moieties in the dye. Some boronic acid-substituted azobenzenes were attached to a polymer or utilized for supramolecular chemistry to produce glucose-selective binding, in which two boronic acid moieties cooperatively bind one glucose molecule. In addition, boronic acid-substituted azobenzenes have been applied not only for glucose monitoring, but also for the sensing of glycated hemoglobin and dopamine.

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Section: Other Target Molecules For Boronic Acid Azobenzenesmentioning

confidence: 99%

Colorimetric Sugar Sensing Using Boronic Acid-Substituted Azobenzenes

2014

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Electrochemical Behaviors of Ferrocene Dicarboxylate and its Application for Heme Detection

Han¹

2017

International Journal of Electrochemical Science

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Heme is an active center of hemoglobin which is a porphyrin compound containing two free carboxyl groups that may exert intermolecular interaction with ferrocene dicarboxylate which can be studied by electrochemical methods. The CV experimental results from this current study showed that ferrocene dicarboxylate had a good pair of redox peaks in NaOH aqueous solution and organic solvents in a quasi-reversible electrode process. There was linear relationship between current and square root of the scan rate, belonging to diffusion-controlled redox processes. The signals from the potential and current had obvious shift after the reaction with heme, especially when the redox peaks disappeared from the organic solvents. Fc(COOH) 2 was activated to give compound Fc(OBt) 2 with saturated structure that did not show better redox peaks due to its worse solubility, hence it didn't form hydrogen bonds with heme. DPV was used for heme assay, where the current was proportional to heme concentration in the range of 1.0-5.0 μM, with 0.7 nM detection limits.

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