The intron-containing gene for yeast profilin (PFY) encodes a vital function.

Abstract

The gene coding for profilin (PFY), an actin-binding protein, occurs as a single copy in the haploid genome of Saccharomyces cerevisiae and is required for spore germination and cell viability. Displacement of one gene copy in a diploid cell by a nonfunctional allele is recessively lethal: tetrad analysis yields only two viable spores per ascus. The PFY gene maps on chromosome XV and is linked to the ADE2 marker. The primary transcript of about 1,000 bases contains an intron of 209 bases and is spliced into a messenger of about 750 bases. The intron was identified by comparison with a cDNA clone, which also revealed the 3' end of the transcript. The 5' end of the mRNA was mapped by primer elongation. The gene is transcribed constitutively and has a coding capacity for a protein of 126 amino acids. The deduced molecular weight of

Reb1p-dependent DNA Bending Effects Nucleosome Positioning and Constitutive Transcription at the Yeast Profilin Promoter

“…One of the actin-binding proteins is profilin (3,(5)(6)(7)(8), which has been thought previously to regulate actin filament formation exclusively by sequestering G-actin monomers and thereby to antagonize actin polymerization (3). However, recent results point to a more complex regulation (9).…”

“…Previously we have isolated and characterized the single structural gene for yeast profilin (PFY1) and shown that its deletion leads to a temperature-conditional phenotype. PFY1 is constitutively transcribed at a moderately high level (8,16,17).…”

Yeast adenylate kinase is transcribed constitutively from a promoter in the short intergenic region to the histone H2A‐1 gene

“…It has been reported for the transcripts of several yeast genes, e.g. AKY2, PFY, ACT1 and CYC1, that their 3'-untranslated ends are quite long and may extend even more than 150 bases beyond the termination signals [22,[30][31][32].…”

“…Debris and nuclei were removed (45 000 x g, 30 min) and the RNA purified by centrifugation through a cushion of CsC1 (n = 1.408) [21]. Primer elongation was performed by annealing a denatured, end-labeled 45 bp HinfI/RsaI fragment (position 16 to 60 of the AKY2 coding sequence [16]) to total RNA and elongating the 3 '-end of the DNA primer by murine reverse transcriptase (Pharmacia-PL, Freihurg) as described [22].…”

Target validation and ligand development for a pathogenic fungal profilin, using a knock‐down strain of pathogenic yeast Candida glabrata and structure‐based ligand design

“…As a potential drug target, the fungal profilins, small actin-binding proteins (Witke, 2004), were focused upon in this study, since these proteins are essential for the growth of most eukaryotic cells, including S. cerevisiae (Haarer et al, 1990;Magdolen et al, 1988;Witke, 2004). Furthermore, the existence of structural information (Eads et al, 1998;Haarer et al, 1993) can strongly support the design of structure-based ligands for profilins.…”