The intravaginal application of misoprostol improves induction of abortion with aglepristone

Ağaoğlu, Ali Reha; Schäfer-Somi, Sabine; Kaya, Doǧan; Küçükaslan, İbrahim; Emre, Birten; Gültiken, Nilgün; Mulazimoglu, Baris S.; Çolak, Abdürrahim; Aslan, Selim

doi:10.1016/j.theriogenology.2011.01.019

Cited by 13 publications

(14 citation statements)

References 41 publications

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“…36 In parallel with these data, clinical studies on women and bitches reported that MIS is more effective when vaginally applied. 10,16,20 The effective MIS dose is reported to range from 100-800 μg for an individual dose and in various regimens in women; 36 there is no consensus on its optimal dose. Misoprostol dosages in this study were determined in accordance with previous canine and human studies, 20,21,38,39 but we opted for the oral route because the vaginal size of cats is not suitable for MIS administration.…”

Section: Discussionmentioning

confidence: 99%

“…After having been used in human medicine, MIS has been tested in veterinary gynaecology for pregnancy termination, induction of parturition or cervical ripening in dogs, ewes, goats, cows and mares. 10,[20][21][22][23][24][25] There are no reports of health risks associated with this drug, and the results have been successful in the aforementioned studies, according to the study authors. The drug can be safely used in dogs and rats in a wide dose margin without causing any acute or chronic systemic dysfunction.…”

Section: Effect Of Oral Misoprostol Alone or In Combination With Aglmentioning

confidence: 99%

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Effect of oral misoprostol, alone or in combination with aglepristone, on mid-term pregnancy termination in cats

Aslan

Önyay

et al. 2018

Journal of Feline Medicine and Surgery

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Objectives This study aimed to determine the efficacy and safety of oral misoprostol (MIS) administration in the induction of mid-term pregnancy termination in cats. Methods Twenty-eight cats that were pregnant for 30-40 days were allocated to four groups. The aglepristone (AGL) group (n = 7) received 10 mg/kg SC aglepristone q24h for two consecutive days. In the AGL+MIS group (n = 7), AGL (as administered in the AGL group) and MIS (200 µg/cat PO q12h until the start of abortion) were administered. The MIS200 (n = 7) and MIS400 groups (n = 7) received MIS (200 or 400 µg/cat misoprostol, respectively) alone PO q12h until the start of abortion. Blood samples were collected at the start of treatment (d0), 4 days after the start of treatment (d4) and on the day of complete abortion/end of administration (dA/d7). Results The efficacy of the treatment was 71.4% in the AGL group, 100% in the AGL+MIS group, 0% in MIS200 group and 57.4% in MIS400 group ( P = 0.004). No significance was found in relation to the interval from treatment to the start/end of abortion and the duration of abortion in all groups. The most observed side effect was vomiting in both groups administered MIS, particularly in the MIS400 group (56.7%). Progesterone (P4) concentrations were reduced during the abortion, but not to basal levels, in all groups. P4 concentrations were significantly lower at dA/d7 in the MIS400 group compared with the AGL and AGL+MIS groups ( P = 0.002). Conclusions and relevance The results obtained from this study showed that low doses of MIS do not induce abortions in cats but increase the effect of AGL. Although higher doses could terminate pregnancies, this also causes intense unwanted side effects. Therefore, the use of MIS alone as an abortifacient in cats is not recommended. For mid-term pregnancy termination in cats, the combination of misoprostol and aglepristone provides a more effective abortifacient than using either of them alone.

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Section: Discussionmentioning

confidence: 99%

Section: Effect Of Oral Misoprostol Alone or In Combination With Aglmentioning

confidence: 99%

Effect of oral misoprostol, alone or in combination with aglepristone, on mid-term pregnancy termination in cats

Aslan

Önyay

et al. 2018

Journal of Feline Medicine and Surgery

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“…Blood samples were centrifuged for 3 min at 3,000 g , and the sera obtained were stored at −18°C until analysis. The concentrations of P4 (ng/ml) and E2 (pg/ml) were measured in serum samples using the electro‐chemiluminescence immunoassay (ECLIA) with a fully automated Cobas Modular E170 Analyzer (Roche Diagnostics, Mannheim, Germany) in an internationally certified commercial laboratory (Düzen Laboratories Group, Ankara, Turkey) as described before (Agaoglu et al., ). Extraction efficiency was >95%.…”

Section: Methodsmentioning

confidence: 99%

Expression of GnRH receptor in the canine corpus luteum, and luteal function following deslorelin acetate‐induced puberty delay

Kaya

Gram

Kowalewski

et al. 2017

Reprod Domestic Animals

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The goals of this study were as follows: (Experiment 1) to examine the basic capability of canine corpora lutea (CL) to respond to GnRH by assessing expression of gonadotropin-releasing hormone receptor (GnRH-R) in luteal samples collected throughout the luteal lifespan from non-pregnant dogs, and (Experiment 2) to investigate the effects of pre-pubertal application of the GnRH agonist deslorelin acetate on luteal function following the first oestrus. Mature CL were collected during the mid-luteal phase (days 30-45) from treated and control bitches. Transcript levels of several factors were determined: estrogen receptors (ESR1/ERα, ESR2/ERβ), progesterone (P4)-receptor (PGR), prolactin receptor (PRLR), PGE2-synthase (PTGES) and PGE2 receptors (PTGER2/EP2, PTGER4/EP4), vascular endothelial growth factor (VEGFA) and VEGF receptors (VEGFR1 and VEGFR2), cyclooxygenase 2 (COX2/PTGS2), steroidogenic acute regulatory protein (STAR) and 3β-hydroxysteroid dehydrogenase (3βHSD). Additionally, levels of Kisspeptin 1 (Kiss1) and its receptor (KISS1-R) were evaluated. Although generally low, GnRH-R expression was time dependent and was elevated during early dioestrus, with a significant decrease towards luteal regression. In deslorelin-treated and control dogs, its expression was either low or frequently below the detection limit. EP2 and VEGFR1 were higher in the treated group, which could be caused by a feedback mechanism after long-term suppression of reproductive activity. Despite large individual variations, 3βHSD was higher in the deslorelin-treated group. This, along with unchanged STAR expression, was apparently not mirrored in increased luteal functionality, because similar P4 levels were detected in both groups. Finally, the deslorelin-mediated long-term delay of puberty does not have negative carry-over effects on subsequent ovarian functionality in bitches.

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“…Samples were stored at −18°C until analysis. The standardized measurement of progesterone (P4) concentrations was performed by Electrochemiluminescence Immunoassay (ECLIA) with the fully automated Cobas Modular E170 Analyzer ® (Roche Diagnostics, Mannheim, Germany) in the international certified Düzen Laboratories Group, Ankara, Turkey) as published before (Agaoglu et al., ). Extraction efficiency was >95%.…”

Section: Methodsmentioning

confidence: 99%

Kisspeptin‐10 and the G protein‐coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells

Schäfer-Somi

Kaya

et al. 2016

Reprod Domestic Animals

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ContentsUterine tissue was collected from bitches after ovariohysterectomy at different times after ovulation. Samples were assigned to four groups: metestrous non-pregnant, day 10-12, n = 4; pre-implantation, day 10-12, n = 9; post-implantation, day 18-25, n = 13; mid-gestation, day 30-40, n = 7. RT-qPCR detection was performed for kiss1 and the G protein-coupled receptor 54 (GPR54, specific receptor for kisspeptin). In 0-300). In non-pregnant and pre-implantation tissues, gene expression was highly variable for kiss1 and GPR54. Expression of GPR54 was higher before embryo adhesion than during post-implantation and mid-gestation (p < .05), whereas there was no difference found between groups for kiss1. Except during the pre-implantation period, KP-10 expression was higher in the non-pregnant uterus compared to all gestational periods investigated, indicating a pregnancy-related downregulation. In the preimplantation period, KP-10 was present in larger vessels only, whereas the presence of GPR54 in vessels was found in all samples, with most labelling in the postimplantation period. KP-10 was present in superficial uterine glands, GPR54 in superficial and deep uterine glands of the post-implantation uterus. In myocytes, the highest staining for KP-10 was seen in the non-pregnant uterus, whereas the highest staining for GPR54 was seen in post-implantation and mid-gestation. Syncytiotrophoblast cells stained for both KP-10 and GPR54 in post-implantation and mid-gestation, with maximum intensity for GPR54 in the latter. We conclude that KP-10 and GPR54 are expressed in the canine uterus and trophoblast cells. However, during pregnancy, expression of both proteins seems to be differentially regulated.

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The intravaginal application of misoprostol improves induction of abortion with aglepristone

Cited by 13 publications

References 41 publications

Effect of oral misoprostol, alone or in combination with aglepristone, on mid-term pregnancy termination in cats

Effect of oral misoprostol, alone or in combination with aglepristone, on mid-term pregnancy termination in cats

Expression of GnRH receptor in the canine corpus luteum, and luteal function following deslorelin acetate‐induced puberty delay

Kisspeptin‐10 and the G protein‐coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells

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