Expression of GnRH receptor in the canine corpus luteum, and luteal function following deslorelin acetate‐induced puberty delay

Kaya, Doǧan; Gram, Aykut; Kowalewski, Mariusz P.; Schäfer-Somi, Sabine; Kuru, Mushap; Boos, Alois; Aslan, Selim

doi:10.1111/rda.13038

Cited by 9 publications

(19 citation statements)

References 44 publications

(54 reference statements)

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“…We conclude that ovarian functionality, serum steroid hormone concentrations and uterine health were normal after delay of puberty with 4.7 or 9.4 mg of deslorelin, all bitches had ovulations and cycled normally (Kaya et al., , ). However, follow‐up studies shall reveal the fertility of these bitches.…”

Section: Discussionmentioning

confidence: 87%

“…This was expected since uterine development should be finalized at the age of 4 months; uterine adenogenesis was reported to be completed at the age of 6‐8 weeks (Cooke et al., ). Furthermore none of the bitches showed any uterine disease which is important for their later breeding fitness; so far, no clinical abnormalities were seen after delay of puberty with GnRH agonists (Fontaine et al., ; Kaya et al., , , ; Marino et al., ; Rubion et al., ; Trigg et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we could show that pre‐pubertal application of one 4.7 or 9.4 mg deslorelin implant was without a negative effect on later ovarian function of bitches (Kaya et al., ). Even though the bitches in the different groups did not have the same body weight and belonged to different breeds, all were truly pre‐pubertal as proven by clinical and cytological examination and hormonal measurement.…”

Section: Discussionmentioning

confidence: 99%

“…Pre‐pubertal treatment with GnRH agonists has been reported in earlier studies; puberty could be successfully delayed in both male and female dogs and cats (Fontaine, Maenhoudt, Mir, Navarro, & Fontbonne, ; Kaya et al., , , ; Marino et al., ; Rubion et al., ; Trigg, Doyle, Walsh, & Swangchan‐uthai, ). Different preparations have been used and different dosages; azagly nafarelin 8.5 mg (Rubion et al., ) or mostly deslorelin 4.7 or 9.4 mg implants (Suprelorin ® , Virbac, F; Trigg et al., ; Fontaine et al., ; Marino et al., ; Kaya et al., ; Kaya et al., , ). The observed duration of effectiveness varied from 8 months to more than 24 months and it seems that it is not only dose dependent.…”

Section: Introductionmentioning

confidence: 86%

“…We therefore started to investigate the local effects on reproductive organs; first on canine luteal function following the first oestrus. When one implant was given at the age of 4 months, no negative effect was seen on the ovaries of bitches, neither macroscopically nor histologically, and ovarian functionality was not impaired (Kaya et al., ). We thereafter were interested to elucidate the effect of long‐term pre‐pubertal GnRH agonist treatment on uterine tissues which was the aim of this study.…”

Section: Introductionmentioning

confidence: 99%

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Pre‐pubertal treatment with a GnRH agonist in bitches—Effect on the uterus and hormone receptor expression

Schäfer-Somi

Kaya

Sözmen

et al. 2018

Reprod Domestic Animals

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Contents Aim of the study was to examine the effect of deslorelin on uterine tissues of eleven pre‐pubertal bitches aged 4.2 ± 0.6 m. Implants containing placebo (sodium chloride 0.9%; n = 4, G I), 4.7 mg (n = 3, GII) or 9.4 mg (n = 4, GIII) deslorelin acetate (Suprelorin®; Virbac, France), were administered subcutaneously. Signs of oestrus, vaginal cytology, serum progesterone (P4) and estradiol‐17β (E2) concentrations were monitored until the occurrence of oestrus. Bitches were ovariohysterectomized and sections from the uterine tissue were subjected to immunohistochemistry (IHC) for detection of GnRH receptor (R), Kisspeptin (KP)10, Kisspeptin receptor (GPR54), androgen receptor (AR), oestrogen receptor (ER) α,β, and progesterone receptor (PR). Tissue sections were scored semi‐quantitatively using an immunoreactivity score (IRS) ranging from 0 to 300 (3). Since some animals were ovariohysterectomized before puberty (n = 1 from GII and n = 2 from GIII), and some in metestrus (all controls and 2 from GII and GIII each), results from these animals were separately evaluated and compared to the controls. Results: No abnormalities were seen in uterine tissues. Kisspeptin 10 expression was low in all cell types, highest IRS were seen in the vascular endothelial cells. The GPR54 was mainly detected in the luminal epithelial cells, superficial and deep uterine glands. The expression of GPR54 and ERα,β was especially high in bitches operated prepubertally. No difference was observed between the controls and experimental bitches operated in their first metestrus. The PR and ERα,β were exclusively expressed in superficial and deep uterine glands and luminal surface epithelial cells. The AR and GnRH‐R expression was negative in all cells of all groups. We conclude that application of 4.7 or 9.4 mg deslorelin at the age of 4 months did not cause uterine disturbances. GPR54 expression might be influenced by pre‐pubertal deslorelin treatment or the changings related to approaching puberty; the latter is supposed in case of ERα,β.

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Section: Discussionmentioning

confidence: 87%