The intestinal absorption of methionine in chickens provided with permanent Thiry-Vella fistulas

Lerner, Joseph; Yankelowitz, S.; Taylor, M. W.

doi:10.1007/bf01897561

Cited by 22 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some authors (Katz & Baker, 1975a;Boebel & Baker, 1982;van Weerden et al 1982van Weerden et al ,1983 suggest that the methionine : cysteine value and the degree of total sulphur amino acid deficiency of the basal diet used can confuse the comparison between DL-HMB and DL-methionine in experiments with practical feedstuffs. A number of workers (Tipton et al 1966;Lerner et al 1969;Baker, 1976) have speculated that DL-HMB is not as well absorbed from the intestinal tract as D-and L-methionine. Dibner & Knight (1984b) have shown that L-methionine and DL-HMB are absorbed through the chick intestinal mucosa by different mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolism of L-methionine, DL-methionine and DL-2-hydroxy 4-methylthiobutanoic acid by broiler chicks

1985

Br J Nutr

View full text Add to dashboard Cite

1. Metabolism, in broiler chicks, of DL-2-hydroxy 4-methylthiobutanoic acid (DL-HMB), DL-methionine and L-methionine was compared in vivo using 14C-labelled tracers.2. The distribution of L-[1-14C]methionine and DL-[1-14C]HMB in the major body tissues was examined for a period of 120 min after administration.3. The relative oxidation (14CO2, exhaled), excretion and incorporation into tissue protein of L-[l-14C]methionine, DL-[l-14C]methionine and DL-[1-14C]HMB were measured in fed birds.4. Tissue distribution of L-[1-14C]methionine and DL-[1-14C]HMB differed during 60–90 min following administration.5. The production of 14CO2, from each of the tracers was similar but excretion of 14C-labelled material was very different with the greatest excretion from DL-[1-14C]HMB and the least from L[1-14C]methionine.6. The incorporation of 14C into tissue proteins varied with the tracer given and the tissue examined. Liver and kidney had equivalent incorporation from each of the tracers while other tissues examined showed lower incorporation from DL-[1-14C]methionine and DL-[1-14C]HMB.7. The results show that DL-HMB, D-methionine and L-methionine are metabolized differently in vivo and that they are excreted in differing proportions. There is also a difference in the ability of each to act as a precursor for protein synthesis in tissues other than liver.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative metabolism of L-methionine, DL-methionine and DL-2-hydroxy 4-methylthiobutanoic acid by broiler chicks

1985

Br J Nutr

View full text Add to dashboard Cite

show abstract

“…Stereochemical Selectivity. One of the most documented characteristics of the amino acid transport system is its ability to distinguish between amino acid stereoisomers (Aroska and Berg, 1962;Clarke gal., 1951;Jervis and Smuth, 1960;Kuroda and Gimbel, 1954;Lerner et al, 1969;Paine~ al., 1959). This characteristic indicates that the amino acid transport is more than diffusion (Kuroda and Gimbel, 1954).…”

Section: Factors Which Affect Amino Acid Absorptionmentioning

confidence: 99%

Characteristics of Threonine, Valine and Methionine Absorption in the Jejunum and Ileum of Sheep

1979

Journal of Animal Science

View full text Add to dashboard Cite

transport systems or the interactions among amino acids for these systems in the ruminant small intestine. In order to increase protein utilization and in turn increase growth, efficiency and production, all aspects of amino acid nutrition of the ruminant should be explored. Experiments were undertaken to determine the amino acid absorption characteristics of three structurally different amino acids at two different intestinal sites. Double re-entrant cannulae were employed to ascertain these characteristics in vivo.

show abstract

“…The three groups demonstrated that the absorption of HMB does not resemble that of an amino acid in several respects. First, Lerner et al (1969) provided data indicating that the rate of absorption of HMB was not the same as that of L-methionine when compared using ileal Thiry-Vella fistulas. In research comparing Lmethionine and HMB in vivo and in vitro, Knight and Dibner (1984) concluded that HMB absorption did not require energy and that its uptake by gastrointestinal epithelial cells was a concentration-dependent process.…”

Section: Introductionmentioning

confidence: 98%

“…The mechanism of absorption of HMB has been studied by Lerner et al (1969), Knight and Dibner (1984), and Saroka and Combs 'Registered trademark of Monsanto Co. (1986). The three groups demonstrated that the absorption of HMB does not resemble that of an amino acid in several respects.…”

Section: Introductionmentioning

confidence: 99%

Absorption of 14 C-2-Hydroxy-4-(Methylthio)Butanoic Acid (Alimet®) from the Hindgut of the Broiler Chick

et al. 1988

View full text Add to dashboard Cite

The purpose of these studies was to examine the role of the hindgut of the broiler chick in the absorption of 2-hydroxy-4-(methylthio)butanoic acid (HMB). When 14C-HMB was delivered directly into the hindgut, the rate of absorption from this gastrointestinal site was about 40% of the administered dose per hour. Plasma radiolabel appearance indicated that the 14C-HMB lost from the hindgut was being absorbed into the bloodstream of the bird. Decarboxylation experiments using cecal microorganisms showed that the loss of 14C-HMB could not be accounted for by bacterial metabolism. When birds were dosed with radiolabelled HMB and tissue samples were tested, results showed that the 14C-HMB that was absorbed from the hindgut was incorporated into protein in a dose-related manner. In addition, an equimolar, equal specific activity intraperitoneal dose of HMB did not alter the rate of HMB adsorption from the hindgut. This indicates that HMB absorption from the gut is not limited by HMB already in the body tissues. This result confirmed that the rate of HMB diffusion into the blood and its conversion to methionine in body tissues were sufficient to maintain the concentration gradient required for the continued absorption of HMB. Finally, whole body autoradiography comparing 35S-HMB and 35S-DL-methionine showed no substantial differences in terms of label density or distribution. These studies demonstrate that 14C-HMB disappears from the lumen of the large intestine and ceca when it is administered directly into the hindgut. This research confirms that HMB is absorbed throughout the entire gastrointestinal system.

show abstract

The intestinal absorption of methionine in chickens provided with permanent Thiry-Vella fistulas

Cited by 22 publications

References 0 publications

Comparative metabolism of L-methionine, DL-methionine and DL-2-hydroxy 4-methylthiobutanoic acid by broiler chicks

Comparative metabolism of L-methionine, DL-methionine and DL-2-hydroxy 4-methylthiobutanoic acid by broiler chicks

Characteristics of Threonine, Valine and Methionine Absorption in the Jejunum and Ileum of Sheep

Absorption of 14 C-2-Hydroxy-4-(Methylthio)Butanoic Acid (Alimet®) from the Hindgut of the Broiler Chick

Contact Info

Product

Resources

About