Nocturnal stomatal conductance (g sn ) represents a significant source of water loss, with implications for metabolism, thermal regulation and water-use efficiency. With increasing nocturnal temperatures due to climate change, it is vital to identify and understand variation in the magnitude and responses of g sn in major crops.We assessed interspecific variation in g sn and daytime stomatal conductance (g s ) in a wild relative and modern spring wheat genotype. To investigate intraspecific variation, we grew six modern wheat genotypes and two landraces under well watered, simulated field conditions.For the diurnal data, higher g sn in the wild relative was associated with significantly lower nocturnal respiration and higher daytime CO 2 assimilation while both species exhibited declines in g sn post-dusk and pre-dawn. Lifetime g sn achieved rates of 5.7-18.9% of g s . Magnitude of g sn was genotype specific 'and positively correlated with g s . g sn and g s were significantly higher on the adaxial surface. No relationship was determined between harvest characteristics, stomatal morphology and g sn , while cuticular conductance was genotype specific. Finally, for the majority of genotypes, g sn declined with age.Here we present the discovery that variation in g sn occurs across developmental, morphological and temporal scales in nonstressed wheat, presenting opportunities for exploiting intrinsic variation under heat or water stressed conditions.