The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst

Bakhtari, Azizollah; Rahmani, Hamidreza; Bonakdar, Elham; Jafarpour, Farnoosh; Asgari, V.; Hosseini, Seyed Morteza; Hajian, Mehdi; Edriss, M. A.; Nasr-Esfahani, Mohammad Hossein

doi:10.1016/j.cryobiol.2014.09.379

Cited by 20 publications

(18 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding was consistent with that reported by Youm, Kim, Jee, & Kim (2017) who found that vitrification did not affect cell counts of hatching/hatched blastocysts. However, a study by Zhang et al (2009) (Bakhtari et al, 2014;Yao et al, 2017). Another important finding was that qRT-PCR analysis discovered interruptions in the expressions of miR-29a/29b and Dnmt3a/3b genes after IVF and vitrification.…”

Section: Discussionmentioning

confidence: 99%

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Movahed

Soleimani

Hosseini

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Proper epigenetic modifications during preimplantation embryo development are important for a successful pregnancy. We aim to investigate the putative influence of in vitro fertilization (IVF) and vitrification on DNA methylation in mouse preimplantation embryos. The study groups consisted of blastocyst‐derived vitrified two‐cell embryos, nonvitrified embryos, and a control group of in vivo derived blastocysts. We assessed developmental competence, global DNA methylation, relative expression levels of miR‐29a/29b, and their target genes, Dnmt3a/3b. Vitrified embryos had a lower developmental rate as compared with nonvitrified embryos. There was no significant decrease in blastocyst cell numbers among studied groups, whereas there was a steady decline in DNA methylation after IVF and vitrification. The levels of miR‐29a/29b upregulated in the experimental groups as compared with the control group. IVF and vitrification caused Dnmt3a/3b downregulations in blastocysts. The results of this study have suggested that a relationship exists between IVF and embryo vitrification with methylation interruptions in the blastocysts.

show abstract

Section: Discussionmentioning

confidence: 99%

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Movahed

Soleimani

Hosseini

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…Many authors have reported abnormal gene expression and epigenetic events attributable to superovulation in the mouse model. Inappropriate acquisition of epigenetic marks before ovulation induces a decrease in global DNA methylation in the maternal pronucleus of zygotes and in the acetylation of H3K9/14, resulting in dysregulated gene expression at the blastocyst stage (Bakhtari et al, ; Huffman, Pak, & Rivera, ). In addition, it has been found that maternal and paternal H19 methylation is also perturbed by superovulation (Market‐Velker, Zhang, Magri, Bonvissuto, & Mann, ).…”

Section: Embryonic Responses To Art‐induced Stress In the Mouse Modelmentioning

confidence: 99%

Embryo responses to stress induced by assisted reproductive technologies

Ramos‐Ibeas

Heras

Gómez-Redondo

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

Assisted reproductive technology (ART) has led to the birth of millions of babies. In cattle, thousands of embryos are produced annually. However, since the introduction and widespread use of ART, negative effects on embryos and offspring are starting to emerge. Knowledge so far, mostly provided by animal models, indicates that suboptimal conditions during ART can affect embryo viability and quality, and may induce embryonic stress responses. These stress responses take the form of severe gene expression alterations or modifications in critical epigenetic marks established during early developmental stages that can persist after birth. Unfortunately, while developmental plasticity allows the embryo to survive these stressful conditions, such insult may lead to adult health problems and to long-term effects on offspring that could be transmitted to subsequent generations. In this review, we describe how in mice, livestock, and humans, besides affecting the development of the embryo itself, ART stressors may also have significant repercussions on offspring health and physiology. Finally, we argue the case that better control of stressors during ART will help improve embryo quality and offspring health. K E Y W O R D S assisted reproductive technologies, bovine, embryo, human, mouse, stress

show abstract

“…However, the influence of the vitrification and thawing procedures on embryo quality in a variety of mammals remains unclear. For example, there are many reports showing alterations in gene expression [ 3 , 4 , 5 , 6 ] and zona hardening [ 7 , 8 , 9 ] following vitrification and thawing. In recent years, technical improvements in time-lapse monitoring (TLM) have enabled consecutive observations of embryonic development, revealing novel features of embryonic behavior [ 10 , 11 , 12 ].…”

mentioning

confidence: 99%

“…Vitrification has been proposed to affect embryonic gene expression [ 3 , 4 , 5 , 6 ]. However, there has been no report confirming a difference in gene expression according to the presence of contractile behavior.…”

mentioning

confidence: 99%

Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos

Shimoda

Kumagai

Anzai

et al. 2016

Journal of Reproduction and Development

View full text Add to dashboard Cite

Contraction during the blastocyst stage is observed during embryonic development of various mammals, including humans, but the physiological role of this process is not well understood. Using time-lapse monitoring (TLM), we studied the influence of vitrification and contractions on embryonic development in mice. Mouse embryos were cultured at the 2-cell stage. At the 8-cell stage, embryos were randomly divided into a fresh group (FG) and vitrified group (VG) and observed for up to 144 h. Strong contractions (i.e., contractions causing a decrease in volume of more than 20% and expansion of the perivitelline space) occurred significantly more often in unhatched embryos than hatching embryos in both groups. Regarding hatching embryos, contractions in the pre-hatching stage were significantly more frequent in the VG than the FG. Furthermore, mRNA expression levels of genes related to contractions were determined at three time points, the 8-cell stage, early blastocyst stage, and 20 h after blastocoel formation, with quantitative reverse transcription-polymerase chain reaction. There was no significant difference in Hspa1a expression between the FG and VG, but Hspa1a overexpression was observed just after thawing and tended to decrease gradually thereafter in some blastocysts. Furthermore, in the VG, Atp1a1 tended to show higher expression in the strong contraction group than in the weak contraction group. Overall, vitrification is an excellent method for cryopreservation but could increase contractions in the pre-hatching stage and may increase energy demands of the embryo. Observation of contraction by TLM may improve the evaluation of embryo quality.

show abstract

The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst

Cited by 20 publications

References 49 publications

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Embryo responses to stress induced by assisted reproductive technologies

Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos

Contact Info

Product

Resources

About