The Interaction of Sodium and Zinc in the Priming of T Cell Subpopulations Regarding Th17 and Treg Cells

Dünkelberg, Sophie; Maywald, Martina; Schmitt, Axel K.; Schwerdtle, Tanja; Meyer, Sören; Rink, Lothar

doi:10.1002/mnfr.201900245

Cited by 10 publications

(7 citation statements)

References 59 publications

(140 reference statements)

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“…Also, it has been found that zinc can induce IFNγ expression, while IL2 and IL10 did not affect it. 23 , 29 , 30 …”

Section: Discussionmentioning

confidence: 99%

“…Also, it has been found that zinc can induce IFNγ expression, while IL2 and IL10 did not affect it. 23,29,30 Graft versus host disease is a major challenge, and cytokine levels, such as IFN-γ, are sensitive indicators of graft rejection in MLC, hence correlating with the severity of acute GVHD. [31][32][33] For that reason, zinc can suppress allogeneic immune responses because of its ability to stabilize induced Tregs.…”

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confidence: 99%

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Zinc Modulates the Priming of T Helper 1, T Helper 17, and T Regulatory Cells in Allogeneic and Autologous in vitro Models

Alrashidi

Alotiby

2022

JIR

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Introduction Zinc is essential for the growth and differentiation of immune cells. Zinc insufficiency affects immune system function, thereby increasing infection susceptibility, autoimmunity, and allergies. Here, we aimed to determine the effects of zinc supplementation on T cell subpopulations, regulatory T (Tregs), T helper 1 (Th1), and T helper 17 (Th17) cells, in mixed lymphocyte cultures (MLC). Methods Allogeneic immune reactions were imitative using mixed lymphocyte cultures, followed by incubation with zinc to further monitor their effects. Cells were analyzed by flow cytometry. Production of Interferon-gamma (IFNγ), Interleukin-17 A (IL17A), and IL10 were analyzed by enzyme-linked immunosorbent assay. Th1 cell-specific Tbet, Th17 cell-specific RORC2, and Tregs-specific Foxp3 expression levels were determined by quantitative real-time PCR. Results Zinc supplementation at a physiological dose significantly increased CD4+ Foxp3+ Tregs and CD25+ Foxp3+ Tregs numbers and slightly decreased CD4+ RORC2+ and CD25+ RORC2+ Th17 cell numbers. A significant reduction in IFNγ production was observed in both restimulated T cells with autologous peripheral blood mononuclear cell (PBMC) and allogeneic PBMC compared to that in untreated T cells. Zinc significantly reduced IL17 expression, but the increase in IL10 expression was insignificant. In zinc-supplemented MLC, a non-significant decrease in Th1 or Th17 cell-specific transcription factors expression was observed, whereas there was a significant increase in Tregs-specific transcription factor expression. Conclusion Zinc can stabilize Tregs participating in adverse immune reactions or in an in vitro transplantation model.

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“…Also, it has been found that zinc can induce IFNγ expression, while IL2 and IL10 did not affect it. 23 , 29 , 30 …”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Zinc Modulates the Priming of T Helper 1, T Helper 17, and T Regulatory Cells in Allogeneic and Autologous in vitro Models

Alrashidi

Alotiby

2022

JIR

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“…Activation of the channels increased the Na + concentration outside the cell as a result of cell depolarization with simultaneous Zn 2+ influx into the cell [ 32 ]. Zinc acts contrary to sodium [ 33 ]. Is suggested that a sodium-dependent mechanism may control renal tubular reabsorption of zinc [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Is suggested that a sodium-dependent mechanism may control renal tubular reabsorption of zinc [ 34 ]. Dünkelberg et al [ 33 ] found that sodium may influence the zinc concentration by upregulation of ZIP10 expression.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

2021

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Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc–prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol®. The solution was modified with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, sodium, potassium, creatinine and total protein were determined. Zinc added to the Biolasol® composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons. In turn, Zn2+ added to Biolasol + PRL (PRL: 0.1 µg/L) acted as a prolactin inhibitor. We do not recommend the addition of Zn(II) (1 µg/L) and Zn(II) (1 µg/L) + PRL (0.1 µg/L) to the Biolasol solution.

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The associations of dietary intake of high sodium and low zinc with gastric cancer mortality: a prospective cohort study in South Korea

et al. 2023

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An experimental study reported that zinc and sodium display opposite effects on immune cells such as regulatory T cells (Tregs) and T helper 17 cells (Th17), resulting in a contrary effect on the immune system (1) . Thus, the authors suggested that excessive dietary sodium intake and simultaneous low zinc intake may cause an altered immune response. Immune cells also have a pivotal role in regulating tumor progression, and reduced immune cells, such as natural killer cells or Tregs, may worsen gastric cancer (GC) mortality (2) . In addition, there are differences in the characteristics of cases by histological type of GC (i.e., age of onset, distribution of sex, GC mortality) (3) . Thus, the influence of dietary factors on histological types of GC may also differ. Therefore, this cohort study investigated the associations between the combination of sodium and zinc intake and GC mortality and whether these associations differ by histological type by following up deaths of GC cases in Korea.A total of 490 patients with GC were enrolled between 2002 and 2006. The inclusion criteria were as follows: 1) age between 20 and 79 years, 2) diagnosis of GC through endoscopic biopsy and histopathologic examination, and 3) available epidemiological and dietary survey data. Survival or death was prospectively followed up until December 31, 2016. Finally, 300 patients with the two main histological types of GC were included; 99 GC deaths occurred during a median follow-up period of 7.1 years. Nutrient and food intake were assessed using a quantitative food frequency questionnaire (FFQ). We used a slightly modified version of the validated FFQ. The risk of GC mortality was assessed using the Cox proportional hazards regression analysis.In all patients, high sodium intake showed a significantly higher GC mortality than low sodium intake (hazard ratio [HR] = 1.67, 95% confidence interval [CI] = 1.05-2.65). Patients with high sodium and low zinc intake had a significantly higher GC mortality than those with low sodium and high zinc intake (HR, 2.07; 95% CI, 1.09-3.93). However, there was no significant difference between the combination of sodium and zinc intake with GC mortality according to the histological type.We found that a combination of high sodium and low zinc intake may worsen the survival rate of patients with the main histological type of GC. Thus, we suggest that intake of low sodium and high zinc (e.g., nuts such as walnuts, peanuts, and pine nuts) may be helpful in improving the survival rate of patients with GC.

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The Interaction of Sodium and Zinc in the Priming of T Cell Subpopulations Regarding Th17 and Treg Cells

Cited by 10 publications

References 59 publications

Zinc Modulates the Priming of T Helper 1, T Helper 17, and T Regulatory Cells in Allogeneic and Autologous in vitro Models

Zinc Modulates the Priming of T Helper 1, T Helper 17, and T Regulatory Cells in Allogeneic and Autologous in vitro Models

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

The associations of dietary intake of high sodium and low zinc with gastric cancer mortality: a prospective cohort study in South Korea

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