Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

Abstract

Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc–prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol®. The solution was modified with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, sodium, potassium, creatinine and total protein were determined. Zinc added to the Biolasol® composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons. In turn, Zn2+ added to Biolasol + PRL (PRL: 0.1 µg/L) acted as a prolactin inhibitor. We do not recommend the addition of Zn(II) (1 µg/L) and Zn(II) (1 µg/L) + PRL (0.1 µg/L) to the Biolasol solution.

Effectiveness Assessment of a Modified Preservation Solution Containing Thyrotropin or Follitropin Based on Biochemical Analysis in Perfundates and Homogenates of Isolated Porcine Kidneys after Static Cold Storage

“…We have shown that prolactin (PRL, dose: 1 µg/L) [51,57], lutropin (LH, dose: 0.01 µg/L) [58], and selenium (Se 4+ , dose: 1 µg/L) [48] have a beneficial protective effect on the kidneys. The addition of Zinc to the Biolasol composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons [59].…”

Effect of Copper on the Function of Isolated Porcine Kidneys Stored Using Simple Hypothermia

“…After this time, the kidney lavage was repeated (0 and 30 min perfusion 2). Markers for indirect assessment of renal function were determined in the perfusate samples collected [ 1 , 2 , 3 ]. After a time of 48 h and 30 min, graft samples were taken for biochemical analysis.…”

“…Renal ischemia in the peri-transplant period causes a number of changes that adversely affect the initiation of normal vital functions in grafts after transplantation. The mechanism of ischemic damage has been described in our previous articles [ 1 , 2 ]. The key point is that ischemia causes a rapid depletion of intracellular reserves of high-energy compounds (ATP).…”

“…Zinc added to Biolasol fluid showed little efficacy in protecting nephrons. In contrast, Zn 2+ added to Biolasol + PRL fluid (0.1 µg/L) acted as a prolactin inhibitor [ 2 ]. In addition, manganese (Mn 2+ : 1 µg/L) did not show antioxidant activity as a component of Biolasol fluid [ 3 ].…”

The Effect of Antioxidant Added to Preservation Solution on the Protection of Kidneys before Transplantation

“…The concentration of malondialdehyde decreased, with a concomitant increase in the antioxidation capacity of plasma [ 48 , 49 ]. Ostróżka-Cieślik et al [ 21 ] also conducted studies on the effect of zinc on the prevention of ischemic damage in isolated porcine kidneys. They modified Biolasol liquid with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L.…”