The interaction of photoactivators with proteins during microfabrication

Lafferty, Joseph; Strande, James R.; Kerns, Peter; Fox, Nathan; Basu, Swarna

doi:10.1016/j.jphotochem.2013.10.017

Cited by 4 publications

(5 citation statements)

References 33 publications

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“…Recent studies have shown that the Rose Bengal molecule, which shows similar binding characteristics to transition metal complexes, can interact (dock) at a site that is less than 3Å from Trp-212 in BSA with a binding energy of −26 kJ/mol [40]. Unpublished docking data from our own group also show similar binding energies between various small nickel complexes and the BSA protein.…”

Section: Quantum Chemical Calculationssupporting

confidence: 52%

Static quenching of tryptophan fluorescence in proteins by a dioxomolybdenum(VI) thiolate complex

Rhodes

Swartz

Hosler

et al. 2014

Journal of Photochemistry and Photobiology A: Chemistry

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Section: Quantum Chemical Calculationssupporting

confidence: 52%

Static quenching of tryptophan fluorescence in proteins by a dioxomolybdenum(VI) thiolate complex

Rhodes

Swartz

Hosler

et al. 2014

Journal of Photochemistry and Photobiology A: Chemistry

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“…Upon conjugation of RB with Lys-Au NCs, the RB absorption was observed at approximately 550 nm, in addition to absorption at less than 450 nm by Lys-Au NCs; the absorbance of RB in the conjugates increased with the ratio of RB to Lys-Au NCs (Figure B). This effect indicates the conjugation of Lys-Au NCs and RB via the interaction of Lys and RB . From the DLS measurements, the size ( D ) of the Lys-Au NCs/RB conjugate (Lys-Au NCs: RB = 0.3:1) was estimated to be 12 nm, which is larger than that of Lys alone (D ≈ 4 nm).…”

Section: Results and Discussionmentioning

confidence: 96%

“…This effect indicates the conjugation of Lys-Au NCs and RB via the interaction of Lys and RB. 28 From the DLS measurements, the size (D) of the Lys-Au NCs/RB conjugate (Lys-Au NCs: RB = 0.3:1) was estimated to be 12 nm, which is larger than that of Lys alone (D ≈ 4 nm). This increase presumably reflects the formation of Lys-Au NCs/RB by conjugation.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates

et al. 2021

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Antibacterial photodynamic therapy (aPDT) utilizes reactive oxygen species such as singlet oxygen ( 1 O 2 ) and free radicals via photosensitizers, which are light and light-sensitive agents, to reduce bacterial infections. It has been utilized as a treatment for dental diseases in place of antibiotic therapies. However, aPDT does not always cause the desired therapeutic effect due to the instability of organic photosensitizers and the formation of bacterial biofilms. To promote the antibacterial and antibiofilm effects of aPDT, we have proposed a lysozyme (Lys)-gold nanoclusters (Au NCs)/rose bengal (Lys-Au NCs/RB) conjugate as a novel photosensitizer. This conjugate was found to effectively impede the growth of both gram-positive and gram-negative bacteria when exposed to white light-emitting diode (LED) irradiation. The photoexcited Lys-Au NCs/RB showed significantly higher antibacterial activity than photoexcited Lys-Au NCs or RB alone. The synergistic effect is a result of the combination of Lys (an antibacterial protein) and enhanced 1 O 2 generation related to resonance energy transfer (RET) in the Au NCs/RB conjugate. Photoexcited Lys-Au NCs/RB increased the effects of aPDT in a dose- and time-dependent manner. Furthermore, the photoexcited Lys-Au NCs/RB successfully decreased Streptococcus mutans biofilm formation. However, in contrast, it did not have a negative effect on the proliferation, adhesion, or spread of mammalian cells, indicating low cytotoxicity. Lys-Au NCs/RB is a novel photosensitizer with low cytotoxicity that is capable of bacterial inactivation and the suppression of biofilm formation, and could help to improve dental treatments in the future.

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“…xanthene photoactivator that has been used to cross-link proteins on the micron and sub-micron scale [5,6,7]. Rose Bengal has a high absorption cross-section and extinction coefficient [8].…”

Section: Resultsmentioning

confidence: 99%

“…Various data analysis approaches have been implemented and compared. This is an upgrade to a microscope that is already used for imaging (white-light) and photopolymerization [5,6]. This will enable us to carry out experiments on low-volume (<100μL) samples and track subtle fluorescence changes in processes like FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).…”

Section: Introductionmentioning

confidence: 99%

A cost-effective fluorescence detection system for pulsed laser analysis

et al. 2015

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A cost-effective fluorescence detection system has been developed using a National Instruments PCI-6251 data acquisition (DAQ) board that is driven by LabVIEW Signal Express. The signal is collected using a collimator and transported to the DAQ board using a fiber-coupled detector. The same detection system has been incorporated into an inverted microscope fitted with internal dichroic mirrors to provide a cost-effective alternative to commercial fluorescence microscopes. The detection system has been used to measure fluorescence intensity and generate a laser power curve that can be used for pulsed lasers. Different data analysis approaches have been compared and standard deviation and signal-to-noise ratios have been determined.

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The interaction of photoactivators with proteins during microfabrication

Cited by 4 publications

References 33 publications

Static quenching of tryptophan fluorescence in proteins by a dioxomolybdenum(VI) thiolate complex

Static quenching of tryptophan fluorescence in proteins by a dioxomolybdenum(VI) thiolate complex

Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates

A cost-effective fluorescence detection system for pulsed laser analysis

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