The interaction of native DNA with iron(III)-N,N′-ethylene-bis(salicylideneiminato)-chloride

Silvestri, Arturo; Barone, Giampaolo; Ruisi, Gìuseppe; GIUDICE, M. T. LO; Tumminello, Salvatore

doi:10.1016/j.jinorgbio.2004.01.010

Cited by 136 publications

(41 citation statements)

References 44 publications

(78 reference statements)

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“…In fact, an absorption band at about 370 nm has been also observed in 1 mM Tris-HCl aqueous solutions of the isolated ligand. Moreover, the similarity with the UV absorption spectrum of the Fe(III)SalenCl complex [31,32], recently investigated [20], allows us to infer that both the Zn(II) and Cu(II) cationic complexes analogously adopt a square planar O 2 N 2 coordination. The UV-vis absorption spectrum of both metal complexes is significantly perturbed by the addition of increasing amounts of DNA.…”

Section: Uv-vis Absorption Spectroscopymentioning

confidence: 85%

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The interaction of native DNA with Zn(II) and Cu(II) complexes of 5-triethyl ammonium methyl salicylidene orto-phenylendiimine

Silvestri

Barone

Ruisi

et al. 2007

Journal of Inorganic Biochemistry

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106

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The interaction of native calf thymus DNA with the Zn(II) and Cu(II) complexes of 5-triethyl ammonium methyl salicylidene ortophenylendiimine (ZnL 2+ and CuL 2+ ), in 1 mM Tris-HCl aqueous solutions at neutral pH, has been monitored as a function of the metal complex-DNA molar ratio by UV absorption spectrophotometry, circular dichroism (CD) and fluorescence spectroscopy. The results support for an intercalative interaction of both ZnL 2+ and CuL 2+ with DNA, showing CuL 2+ an affinity of approximately 10 times higher than ZnL 2+ . In particular, the values of the binding constant, determined by UV spectrophotometric titration, equal to 7.3 · 10 4 and 1.3 · 10 6 M À1 , for ZnL 2+ and CuL 2+ , respectively, indicate the occurrence of a marked interaction with a binding size of about 0.7 in base pairs. The temperature dependence of the absorbance at 258 nm suggests that both complexes strongly increase the DNA melting temperature (Tm) already at metal complex-DNA molar ratios equal to 0.1. As evidenced by the quenching of the fluorescence of ethidium bromide-DNA solutions in the presence of increasing amounts of metal complex, ZnL 2+ and CuL 2+ are able to displace the ethidium cation intercalated into DNA. A tight ZnL 2+ -DNA and CuL 2+ -DNA binding has been also proven by the appearance, in both metal complex-DNA solutions, of a broad induced CD band in the range 350-450 nm. In the case of the CuL 2+ -DNA system, the shape of the CD spectrum, at high CuL 2+ content, is similar to that observed for w-DNA solutions. Such result allowed us to hypothesize that CuL 2+ induces the formation of supramolecular aggregates of DNA in aqueous solutions.

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Section: Uv-vis Absorption Spectroscopymentioning

confidence: 85%

“…We have previously reported on the interaction of native calf thymus DNA with Fe III (Salen)Cl [20]. The results obtained have been interpreted in terms of an electrostatic binding of [Fe(Salen)] + cation and the phosphate groups of DNA.…”

Section: Introductionmentioning

confidence: 99%

The interaction of native DNA with Zn(II) and Cu(II) complexes of 5-triethyl ammonium methyl salicylidene orto-phenylendiimine

Silvestri

Barone

Ruisi

et al. 2007

Journal of Inorganic Biochemistry

Self Cite

106

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“…Fe was determined by flame atomic absorption spectroscopy, using a Perkin-Elmer 372 instrument. The Mössbauer spectrometer and the data reduction method have been described earlier [20,21]. The isomer shift was reported with respect to the centroid of a a-Fe absorber spectrum at room temperature.…”

Section: Methodsmentioning

confidence: 99%

The interaction of native calf thymus DNA with FeIII-dipyrido[3,2-a:2′,3′-c]phenazine

Terenzi

Barone

Silvestri

et al. 2009

Journal of Inorganic Biochemistry

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a b s t r a c tThe mono and bis dipyrido[3,2-a:2 0 ,3 0 -c]phenazine (dppz) adducts of iron(III) chloride, i.e. [Fe(dppz)]Cl 3 and [Fe(dppz) 2 ]Cl 3 , have been synthesized and characterized. The interaction of the Fe III dppz hydrolyzed aquo complex with native calf thymus DNA has been monitored as a function of the metal complex-DNA molar ratio, by variable temperature UV absorption spectrophotometry, circular dichroism (CD) and fluorescence spectroscopy. The results obtained in solution at various ionic strength values give support for a tight intercalative binding of the Fe III dppz cation with DNA. In particular, the appearance of induced CD bands, caused by the addition of Fe III dppz, indicate the existence of a rigid metal complex-DNA-binding leading to dominating chiral organization of Fe III dppz species within the DNA double helix. The trend of selected CD bands with the molar concentration of Fe III dppz emphasizes that the presence of high amounts of metal complex induces also the formation of DNA-Fe III dppz supramolecular aggregates in solution. The analysis of fluorescence measurements allowed us to calculate a value of the intercalative binding constant comparable to that obtained by UV spectrophotometric titration. Finally, the temperature dependence of the absorbance at 258 nm shows that the metal complex strongly increases the DNA melting temperature already at metal complex-DNA molar ratio equal to 0.25 suggesting that metal complex intercalation effectively hinders DNA denaturation. Overall, the results of the present study point out that the Fe III dppz aquo complex has DNA-binding properties analogous to those previously reported for the tris-chelate Fe II (phen) 2 dppz complex (phen = 1,10-phenantroline).

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“…The use of Schiff bases as ligands in the formation of transitionmetal complexes has been extensively studied owing to their intrinsic properties, such as versatile spectroscopic and electrochemical responsive receptors for charged and neutral guests of these systems [1][2][3]. Interactions of biologically active metal ion such as copper(II) with L-amino-acid derivatives and peptide derivatives are of considerable interest as these often represent the behavior of metal ions in the enzymes [4][5][6][7].…”

Section: Discussionmentioning

confidence: 99%

Crystal structure of diaqua-(μ-4,4′-bipyridine)-bis(N-salicylideneaspartato)- dicopper(II), Cu2(H2O)2(C11H9NO5)2(C10H8N2)

Feng¹,

Li²,

Han³

et al. 2007

Zeitschrift Für Kristallographie - New Crystal Structures

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Source of materialA mixture of 4,4¢-bipyridine (0.96 g, 0.5 mmol) in copper(II) diacetate monohydrate (0.102 g, 0.5 mmol) and anhydrous methanol (10 mL) was refluxed for 20 min. Then 20 mL methanol solution of freshly distilled salicylaldehyde and aspartic amino acid were added simultaneously. The mixture was stirred for 3 h, then filtered. The resulting clear solution was diffused with diethyl ether vapor at room temperature for two weeks, blue blockshaped crystals were formed, collected by filtration and dried in air (yield 0.411 g, 52 %). Elemental analysis found: C, 49.04 %; H, 3.73 %; N, 7.54 %; calc. for C32H30N4O12Cu2: C, 48.63 %; H, 3.80 %; N, 7.09 %. Experimental detailsWhile the H atoms of water molecules were refined, the other H atoms were added geometrically. DiscussionThe use of Schiff bases as ligands in the formation of transitionmetal complexes has been extensively studied owing to their intrinsic properties, such as versatile spectroscopic and electrochemical responsive receptors for charged and neutral guests of these systems [1][2][3]. Interactions of biologically active metal ion such as copper(II) with L-amino-acid derivatives and peptide derivatives are of considerable interest as these often represent the behavior of metal ions in the enzymes [4][5][6][7]. Salicylaldehyde Schiff base derived from amino acids and peptides offer two or more potential binding sites for metal ions.The asymmetric unit consists of one half of the title complex unit [Cu 2(sal-asp)2(bipy)(H2O)2]. The two metal-centered pyramids of the dinuclear complex are bridged by the 4,4¢-bipy molecule with a Cu1···Cu2 separation of 11.112 Å. In the complex, the salicylaldehyde-aspartic Schiff base exists as a dianion including the carboxyl group, shows a tridentate coordination mode as observed in the analogous ternary polyhedron around the Cu(II) ion which can be described as a roughly distorted square pyramid. The square base is formed by the nitrogen atom of the deprotonated aspartic amino acid, a-aspartic acid carboxyl group oxygen atom and a phenol-hydroxyl oxygen atom from salicylaldehyde derivate and the heterocyclic nitrogen atom of 4,4¢-bipy. The coordination sphere about Cu(II) ion is apically completed by a bonded oxygen of a water molecule. The CuO and CuN bond lengths in the basal square plane, being in the ranges of 1.906(2) Å 2.016(2) Å and 1.942(2) Å 2.006(2) Å, respectively, are close to those found in copper(II) complexes of N-deprotonated amino acids with an N3O2 group or N 2 O 2 group [8,9]. The two water molecules are coordinated to two Cu(II) in opposite direction, which is maybe due to the large steric hindrance of 4,4¢-bipyridines and the salicylaldehyde derivate. The O6Cu1N1 bite angle (99.16 (8) Acknowledgments.

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The interaction of native DNA with iron(III)-N,N′-ethylene-bis(salicylideneiminato)-chloride

Abstract: The interaction between native calf thymus deoxyribonucleic acid (DNA) and

Cited by 136 publications

References 44 publications

The interaction of native DNA with Zn(II) and Cu(II) complexes of 5-triethyl ammonium methyl salicylidene orto-phenylendiimine

The interaction of native DNA with Zn(II) and Cu(II) complexes of 5-triethyl ammonium methyl salicylidene orto-phenylendiimine

The interaction of native calf thymus DNA with FeIII-dipyrido[3,2-a:2′,3′-c]phenazine

Crystal structure of diaqua-(μ-4,4′-bipyridine)-bis(N-salicylideneaspartato)- dicopper(II), Cu2(H2O)2(C11H9NO5)2(C10H8N2)

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