The Interaction of lncRNA-HEIH and lncRNA-HULC with HBXIP in Hepatitis B Patients

Abstract

Hepatitis B virus (HBV) infection is a major risk factor for the development of hepatic cirrhosis (HC) and hepatocellular carcinoma (HCC), which are associated with very high morbidity and mortality rates worldwide. Many studies have shown that long noncoding RNAs (lncRNAs) that are highly expressed in HCC (lncRNA-HEIH) and highly upregulated in liver cancer (lncRNA-HULC) have been implicated in the development and progression of hepatitis B-related HC and HCC. In this study, reverse transcription and quantitative PCR were used to detect the expression of lncRNA-HEIH and lncRNA-HULC and western blot analysis to detect the expression of hepatitis B X-interacting protein (HBXIP). RNA immunoprecipitation was used to detect the interaction of HBXIP with lncRNA-HULC and lncRNA-HEIH. The results showed that lncRNA-HEIH, lncRNA-HULC, and HBXIP were upregulated in hepatitis B patients, particularly those with hepatitis B-related HCC. Both lncRNA-HEIH and lncRNA-HULC interacted with HBXIP. These results suggest that lncRNA-HEIH and lncRNA-HULC interact with HBXIP in hepatitis B-related diseases.

Increased Expression of lncRNA UCA1 and HULC Is Required for Pro-inflammatory Response During LPS Induced Sepsis in Endothelial Cells

“…However, beyond a few studies (Singh et al, 2016; Chowdhury et al, 2017), including one which showed that the lncRNAs EGO (NONHSAT087634) and HOTAIRM1 (NONHSAT119666) are alternatively spliced and that expression of lnc-IL7R is increased during LPS induced sepsis (Chowdhury et al, 2017), not much is known about how altered lncRNA expression is related to pathophysiology of sepsis. HULC (highly upregulated in liver cancer) is a 16 kb lncRNA at chromosomal location 6p24.3 and is has been shown to be overexpressed in patients with liver cancer (Ruan et al, 2018). LncRNA urothelial carcinoma-associated 1 (UCA1) at chromosomal location 19p13.12 was initially identified in human bladder carcinoma but plays widespread role in inflammation and tumorigenesis (Liu et al, 2019).…”

Long noncoding RNA GAS5 does not regulate HBV replication

“…In HBV infection, it is known that several lncRNAs are involved in the development or progression of HBV‐associated HCC (eg, MALAT1, UCA1, DBH‐AS1, Dreh, LINE1, Ftx, and HOTAIR) or were upregulated in HBV infected patients (eg, lncRNA‐HEIH, lncRNA‐HULC, and HBXIP). To understand lncRNAs which likely affect virus replication in host cells, we focused on growth arrest‐specific transcript 5 (GAS5), a critical regulator in tumorigenesis.…”

Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells

“…In clinical studies, HBXIP was elevated in normal gastric mucosa, as well as in gastric cancer tissue and adjacent, noncancerous tissue ( 30 ). Other studies have also confirmed that HBXIP is highly expressed in both liver cancer and ovarian cancer ( 31 , 32 ). These observations indicate that HBXIP plays an important role in the occurrence, invasion, and metastasis of malignant tumors.…”