The interaction of human plasma albumin and reduced glutathione

Synthesis of a mixed disulfide of egg white lysozyme and glutathione ‐ a model substrate for enzymatic reduction of protein mixed disulfides

“…Other protein mixed disultides involving GSH have been prepared from hemoglobin [16-l 81, serum albumin [4,19] , and lens crystallins [20] . These derivatives are less suitable than the lysozyme compound for the study of general reaction pathways of interactions between protein disulfide groups and GSH, since they are not equally well characterized chemically, and in the case of hemoglobin, a high absorbancy limits spectrophotometric measurements.…”

Oxidizing Action of Neonatal and Maternal Plasma on Reduced Glutathione in vitro

“…Libenson and J ena [8,9] have studied the interaction between glutathione and albumin under two different conditions: in an anaero bic environment at a low pH and in an aerobic environment at a nearly neutral pH: in both situations there was formation of mixed disulphides. In the interaction of the second type, after 23 h of incubation, for each mole of albumin about 0.52 moles of GSH were incorporated.…”

Studies of interaction between insulin and glutathione using electrospray ionization mass spectrometry

“…Among them, the interactions of GSH and proteins play significant biological roles in cells including the formation of the mitotic apparatus during cell division and regulation of protein functions and protection of irreversible oxidation of proteins . Therefore, many proteins have been investigated to react with GSH in vitro for the better understanding of mechanisms of interactions of GSH and proteins, for example, serum albumin, bovine pancreatic ribonuclease, eye‐lens protein γ‐crystallin and cytochrome c …”

“…[3,4] Among them, the interactions of GSH and proteins play significant biological roles in cells including the formation of the mitotic apparatus during cell division [5,6] and regulation of protein functions and protection of irreversible oxidation of proteins. [7][8][9] Therefore, many proteins have been investigated to react with GSH in vitro [10][11][12][13][14][15][16][17][18][19][20]23] for the better understanding of mechanisms of interactions of GSH and proteins, for example, serum albumin, [12][13][14] bovine pancreatic ribonuclease, [15,16] eye-lens protein g-crystallin [18,19] and cytochrome c. [20] Insulin is a small globular protein to regulate energy storage and glucose metabolism in the body. It is synthesized in the pancreatic b-cells and causes cells in the liver, muscle and fat tissue to take up glucose from blood, storing it as glycogen in the liver and muscle.…”