The interaction of calcium and strontium with phosphatidyl serine in the anaphylactic secretion of histamine

Foreman, J. C.; Mongar, J. L.

doi:10.1113/jphysiol.1973.sp010200

Cited by 34 publications

(11 citation statements)

References 18 publications

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“…Phosphatidyl seine enhances antigen-evoked histamine secretion in the presence of Ca (Goth, Adams & Knoohuizen, 1971;Mongar & Svec, 1972;Foreman & Mongar, 1973a). The phospholipid increases the rate and magnitude of spontaneous secretion in the presence of Sr, probably by increasing the membrane permeability to Sr.…”

Section: Discussionmentioning

confidence: 99%

“…Histamine secretion evoked by an antigen-antibody reaction on the mast cell membrane is initiated by an increase in membrane permeability to Ca, and the subsequent entry of Ca into the cell from the extracellular environment (Foreman & Mongar, 1973a;Foreman, Mongar & Gomperts, 1973;Dahlqvist, 1974;Foreman et al 1977a). Sr will substitute for Ca in this role (Foreman & Mongar, 1972).…”

Section: Discussionmentioning

confidence: 99%

“…Spontaneous histamine secretion from mast cells occurs independently of extracellular Ca (Foreman & Mongar, 1973a;Foreman, 1973b), but the presence of extracellular Sr leads to an increase in spontaneous secretion, some of the characteristics of which are described in this paper.…”

Section: Introductionmentioning

confidence: 99%

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Spontaneous histamine secretion from mast cells in the presence of strontium

Foreman¹

1977

The Journal of Physiology

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SUMMARY1. Histamine secretion from rat peritoneal mast cells occurs spontaneously in the absence of an external stimulus. Spontaneous secretion increases as the concentration of Sr in the extracellular medium is raised from 1 to 10 m-mole/l. Ca 0-1-10 m-mole/l. does not increase spontaneous secretion.2. Spontaneous histamine secretion in the presence of Sr occurs slowly compared with evoked histamine secretion, reaching a maximum only after more than 120 min incubation with Sr 10 m-molefl. at 370 C and pH 7-6. Phosphatidyl seine, 10 jug/ml., increases the rate of spontaneous secretion in the presence of Sr.3. The spontaneous secretion occurring in the presence of Sr is highly dependent on the extracellular H ion concentration. Maximal secretion occurs at pH and only a very limited secretion is detected at pH below 7-6. The rate of spontaneous secretion is also greater at higher pH. Inhibition of secretion caused by lowering the pH can be reversed by raising the Sr ion concentration over a limited range.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Spontaneous histamine secretion from mast cells in the presence of strontium

Foreman¹

1977

The Journal of Physiology

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“…However, it is not yet established whether calcium uptake follows the process of histamine release or whether histamine release is dependent on calcium uptake. The observed effects of the alkaline earth ions and their interaction with phosphatidyl serine (Foreman & Mongar, 1973) can be explained in terms of a model involving divalent cation gates in the mast cell membrane which, during the passage of calcium and strontium, trigger histamine secretion, but evidence from the direct measurement of calcium movement across the mast cell membrane is still lacking. In smooth muscle it has been shown that lanthanum inhibits the movement of calcium across the cell membrane (van Breemen, 1969;Weiss & Goodman, 1969), and it is conceivable that the ion has a similar action in the mast cell, though it is, of course, possible that lanthanum competes for calcium sites involved in histamine secretion other than those involved with the entry of the ion into the mast cell.…”

Section: Discussionmentioning

confidence: 99%

The action of lanthanum and manganese on anaphylactic histamine secretion

Foreman

Mongar

1973

British J Pharmacology

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SummaryI. In the concentration range 1 nm to 1 /.LM, lanthanum inhibited the calciumdependent component of anaphylactic histamine release, but was without effect on the component which was independent of calcium. 2. The inhibition of anaphylactic histamine release by lanthanum can be reversed by increasing the calcium ion concentration. The pA2 for the lanthanum-receptor interaction was found to be 7-6. 3. Lanthanum also inhibited the activation of anaphylactic histamine release by strontium ions. 4. The inhibition of anaphylactic histamine release by lanthanum was reversed by eluting the lanthanum from the cells. 5. In the concentration range 1 to 300 nM, lanthanum had no effect on the histamine release induced by compound 48/80 in a calcium-free medium, but the effect of calcium on the histamine release by compound 48/80 was antagonized by lanthanum in this range of concentrations. 6. At concentrations of 10 ,uM and greater, lanthanum induced a release of histamine in the absence of antigen. 7. In the concentration range 0 5 to 4 0 mm, manganese inhibited the calciumdependent component of anaphylactic histamine release, but was without effect on the component which is independent of calcium. The inhibition of anaphylactic histamine release by manganese could be reversed by increasing the calcium ion concentration.

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“…The secretion of histamine from mast cells exposed to dextran (1), antigen (2,3) or concanavalin A (Con A) (4,5) is potentiated by phosphatidylserine (PtdSer). Mast cell secretion induced by these secretagogues is not potentiated by other phospholipids such as phosphatidylcholine (PtdCho), phosphatidylinositol, phosphatidylglycerol, phosphatidylethanolamine, or phosphatidic acid (1,4,6,7).…”

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Interaction of phosphatidylserine with mast cells.

Martin¹,

Lagunoff²

1978

Proc. Natl. Acad. Sci. U.S.A.

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Phosphatidylserine (PtdSer) potentiates histamine secretion from mast cells exposed to concanavalin A and Ca2+. In order to identify the form of PtdSer that is responsible for its effect on mast cell secretion, PtdSer containing a tritium-labeled serine moiety (3H-PtdSer) was synthesized from egg yolk phosphatidylcholine. The critical micele concentration (CMC) of 3H-PtdSer and the binding isotherm for 3H-PtdSer interaction with mast cells were determined. The midpoints of the binding isotherm and the dose-response curve for potentiation of secretion coincide and are 2 orders of magnitude greater than the CMC. The shape of the binding curve is explicable either in terms of simple binding of preformed PtdSer micelles or of cooperative binding of monomeric PtdSer in which the number of molecules cooperatively associating with a mast cell binding site is equal to the number of monomers in a PtdSer micelle. In either case, at equilibrium, PtdSer micelles are bound to the mast cells. The number of PtdSer molecules bound to a single mast cell at equilibrium was estimated to be 3.7 X l0s.The secretion of histamine from mast cells exposed to dextran (1), antigen (2, 3) or concanavalin A (Con A) (4, 5) is potentiated by phosphatidylserine (PtdSer). Mast cell secretion induced by these secretagogues is not potentiated by other phospholipids such as phosphatidylcholine (PtdCho), phosphatidylinositol, phosphatidylglycerol, phosphatidylethanolamine, or phosphatidic acid (1, 4, 6, 7). Although phospholipids have been shown to activate membrane-associated enzyme preparations after delipidization (8) as well as such processes as activation of the intrinsic (9) and extrinsic (10) coagulation pathways, to our knowledge the potentiation of mast cell secretion by PtdSer represents the only example of an in situ cellular event that has been shown to be activated by exposure of cells to a specific exogenous phospholipid.As an amphiphilic molecule, PtdSer is capable of undergoing a highly cooperative self-association in which its nonpolar fatty acyl chains are transferred from an aqueous to a hydrocarbon environment. The concentration at which self-association occurs is defined as the critical micelle concentration (CMC) (11). Micellar aggregates,* the geometry of which is determined by the length and number of hydrocarbon chains as well as the polar head group (12), predominate at total amphiphile concentrations in excess of the CMC. It is generally assumed that the CMC of biological phospholipids is sufficiently low (-10-10 M) as to make the contribution of the monomeric form negligible under most experimental conditions. Actually the CMC has been experimentally determined for only one phospholipid class, a homologous series of PtdChos containing saturated hydrocarbon chains (13,14). Precaution must be exercised in extrapolating the data for disaturated PtdCho to anionic phospholipids isolated from tissues. Naturally occurring phospholipids usually contain a large proportion of cis-unsaturated fatty acids and are not homogen...

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The interaction of calcium and strontium with phosphatidyl serine in the anaphylactic secretion of histamine

Cited by 34 publications

References 18 publications

Spontaneous histamine secretion from mast cells in the presence of strontium

Spontaneous histamine secretion from mast cells in the presence of strontium

The action of lanthanum and manganese on anaphylactic histamine secretion

Interaction of phosphatidylserine with mast cells.

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