The action of lanthanum and manganese on anaphylactic histamine secretion

Abstract

SummaryI. In the concentration range 1 nm to 1 /.LM, lanthanum inhibited the calciumdependent component of anaphylactic histamine release, but was without effect on the component which was independent of calcium. 2. The inhibition of anaphylactic histamine release by lanthanum can be reversed by increasing the calcium ion concentration. The pA2 for the lanthanum-receptor interaction was found to be 7-6. 3. Lanthanum also inhibited the activation of anaphylactic histamine release by strontium ions. 4. The inhibition of anaphylactic histamine release by lanthanum was reversed by eluting the lanthanum from the cells. 5. In the concentration range 1 to 300 nM, lanthanum had no effect on the histamine release induced by compound 48/80 in a calcium-free medium, but the effect of calcium on the histamine release by compound 48/80 was antagonized by lanthanum in this range of concentrations. 6. At concentrations of 10 ,uM and greater, lanthanum induced a release of histamine in the absence of antigen. 7. In the concentration range 0 5 to 4 0 mm, manganese inhibited the calciumdependent component of anaphylactic histamine release, but was without effect on the component which is independent of calcium. The inhibition of anaphylactic histamine release by manganese could be reversed by increasing the calcium ion concentration.

Effects of five different organic calcium antagonists on guinea‐pig isolated trachea

“…Although described as a (mineral) calcium antagonist (Takata et al, 1966;Foreman & Mongar, 1973), in our experiments lanthanum chloride had none of the specific inhibitory effects on depolarizing agents observed with organic calcium antagonists. Moreover, it acted only in concentrations much higher than those used, for example, in guinea-pig vas deferens or taenia coli (Weiss & Goodman, 1969;Hay & Wadsworth, 1982).…”

“…In addition, we have compared, in the same preparation, the relaxant effects of calcium antagonists with those of a f-adrenoceptor agonist (isoprenaline), phosphodiesterase inhibitors (theophylline, amrinone; Meisheri et al, 1980;Martorana et al, 1982), parasympatholytic drugs (atropine), anti-histaminics (mepyramine, ketotifen), terfluzine and lanthanum chloride. Terfluzine has been reported to inhibit calmodulin (Stoclet et al, 1981) and lanthanum chloride to antagonize calcium (Takata et al, 1966;Foreman & Mongar, 1973 (Stephens, 1970). Tension was measured isometrically with a Ugo Basil strain gauge and was displayed on Ugo basil pen recorder.…”

Effect of ouabain, digoxin and digitoxigenin on potassium uptake and histamine release from rat peritoneal mast cells

“…This effect may be attributed to displacement by lanthanum of membrane-bound calcium [16], which is considered to be involved in stimulus-secretion coupling [17].…”

The effect of extracellular calcium deprivation on amylase secretion and 45Ca efflux from rat pancreas

“…For example, ACh secretion at the neuromuscular junction (Dodge & Rahamimoff, 1967), anaphylatic histamine release from mast cells (Foreman & Mongar, 1973) and ACh-stimulated catecholamine release from the perfused bovine adrenal gland (Borowitz, 1973) are effectively blocked by reduction of the extracellular Ca2+ concentration to 10-4 M. Also, ACh stimulated amylase secretion from the rat parotid, a gland with many structural similarities to the pancreas, is completely abolished by lowering the extracellular Ca2+ concentration to between 10-3 and 10-4 M (S. Arkle & B. E. Argent, unpublished observations). Different types ofsecretary cell may use different sources ofCa2+ for stimulus-secretion coupling in a manner analogous to that found for excitation-contraction coupling in different types of muscle.…”