The interaction between PmHtrA2 and PmIAP and its effect on the activity of Pm caspase

Saleeart, Anchulee; Mongkorntanyatip, Karntichar; Sangsuriya, Pakkakul; Senapin, Saengchan; Rattanarojpong, Triwit; Khunrae, Pongsak

doi:10.1016/j.fsi.2016.06.013

Cited by 3 publications

(1 citation statement)

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“…Inhibitor of apoptosis protein (IAP), acting as the anti-apoptosis factor, was identified from Penaeus monodon and Litopenaeus vannamei [ 23 , 24 ]. The mechanism of action by Pm-IAP was found to interact with high temperature requirement A2 (HtrA2) and Caspases [ 25 ]. Among these, the Chinese mitten crab Eriocheir sinensis is an important freshwater aquatic economic species, and studying on cell apoptosis mechanism in E. sinensis may push the aquaculture performance of this species forward.…”

Section: Introductionmentioning

confidence: 99%

Roles of three Es-Caspases during spermatogenesis and Cadmium-induced apoptosis in Eriocheir sinensis

Yang

2018

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Functions of Caspases remain obscure in Crustacea. We studied the existence and participations of apoptosis-related factors in Eriocheir sinensis testis. Three Es-Caspases (Es-Caspase 3/ 7/ 8) in E. sinensis were cloned and characterized. We observed that three es-caspases mRNA had specific expression patterns during spermiogenesis, with weak signal around the nucleus and invaginated acrosomal vesicle in early-stage spermatids, became stronger in middle-stage, finally focused on the acrosomal tube and nucleus in mature sperm. We then investigated the immunostaining intensity and positional alterations of Es-Caspase 3, Es-Caspase 8 and p53 during spermatogenesis, which were correlated with the differential tendencies of cells to undergo apoptosis and specific organelles shaping processes. After apoptotic induction by Cadmium, Es-Caspase 8 increased gradually, while Es-Caspase 3 increased firstly and then decreased, Es-p53 initially decreased and then increased. These results implies that Es-Caspase 3/ Es-Caspase 8/ p53 may play roles in Cadmium-induced apoptosis during spermatogenesis, and Caspase 8-Caspase 3-p53 pathway may interact with extrinsic or intrinsic pathways to regulate the destiny of sperm cells. Our study revealed the indispensable roles of Caspases during spermatogenesis and the possible molecular interactions in response to the Cadmium-induced apoptosis in E. sinensis, which filled the gap of apoptotic mechanisms of crustacean.

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Section: Introductionmentioning

confidence: 99%